[PDF] Top 20 Novel Swine Virulence Determinant in the Left Variable Region of the African Swine Fever Virus Genome

... the swine virulence determinant and macrophage host range ...that swine virulence determinant and macrophage host range determinant may have related functions in main- ... See full document

... the genome at previous passages were observed in ...ASFV-G/VP80 genome were also observed in the genome of ASFV-G/VP110 (Table ...the left variable end of the genome observed at ... See full document

... 2008/2 genome possessed a novel allele state at a previously undescribed intergenic repeat locus between genes C315R and ...observed variable repeat sequence polymorphism reported among isolates from ... See full document

... a novel gene with no similarity to other genes or known protein motifs in the current genetic ...identified virulence-associated gene NL-S ...viral genome we have observed that contains both UK and ... See full document

... 1046 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The thymidine kinase TK gene of African swine fever virus ASFV was located within the viral genome by using two deg[r] ... See full document

... African swine fever virus (ASFV) encodes two polyprotein precursors named pp220 and pp62 that are sequentially processed during viral infection, giving rise to six major structural ... See full document

... African swine fever virus (ASFV) is a large DNA virus that enters host cells after receptor-mediated endocytosis and depends on acidic cellular compartments for productive ... See full document

... A detailed analysis, including in vitro translation of mRNAs isolated from infected Vero cells, revealed that the L270 gene belongs to a small subset of early genes, designated immediate[r] ... See full document

... ASF virus (3) and that gold-labeled antibodies recognizing p73 bind to electron-dense layers out- side the inner membrane of ASF virus ...ASF virus does, however, have concentric membrane layers ... See full document

... The African swine fever virus (ASFV)-encoded CD2v transmembrane protein is required for the hemad- sorption of red blood cells around infected cells and is also required for the inhibition of ... See full document

... To determine the subcellular localization of ASFV-DP, it was first cloned with a hemagglutinin (HA) tag under the control of the specific viral promoter in a pcDNA plasmid. COS-7 cells were transfected with pASFV-DP-HA and ... See full document

... region for all eight viruses. All contained both a short NL ORF encoding either 70, 71, or 72 amino acids and ORF UK. Pre- dicted amino acid sequences of these NL-S ORFs are shown in Fig. 2. Pathogenic isolates ... See full document

... and virus progeny yields of these mutants assessed in primary swine macrophage cultures, a CSFV natural target cell, were comparable to that of parental BICv, except mutant N3v (with the N185A mutation), ... See full document

... Conclusions. The ASFV is a large complex DNA virus encod- ing ⬎ 150 proteins. Experimental subunit vaccines based on a few of these antigens have generated different protective outcomes, demonstrating that these ... See full document

... peptides. (A) Secondary structures measured by CD. The structural compo- nents of spFPII.wt, spFPII.2, and spFPII.5 (black, red and blue bars, respec- tively) were calculated for the CD spectra obtained in buffer ... See full document

... the swine macrophage, its main target cell, by using highly purified virus stocks and a combina- tion of flow cytometry, fluorescence microscopy, electron microscopy (EM), live-cell imaging, targeted small ... See full document

... control virus replication results in rapid ASFV replication and induction of hemorrhagic pathology in infected ...rapid virus replication and that deletion of genes that inhibit this induction can attenuate ... See full document

... To understand the structural basis for this observed promiscuity of A179L, we determined the crystal structures of A179L bound to peptides that span the BH3 motifs of Sus scrofa (boar) Bid and Bax BH3. Our analysis ... See full document

... some cases, display small amounts of core material apparently bound to the internal envelope and are very similar to the particles produced when the expression of polyprotein pp220 is repressed (Fig. 6F), and (ii) ... See full document

... the virus factory, could participate in such a late viral BER ...in virus replication is unlikely, since the enzyme is not required for virus growth in Vero ... See full document