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[PDF] Top 20 Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

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Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples

... In the management of HAT, one of the most important tasks is to accurately distinguish the late, encephalitic stage of HAT from the early, hemolymphatic stage. Accurate staging of HAT is critical because failure to treat ... See full document

6

Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis

... and Trypanosoma brucei ...dipstick detection technique, Oligochromatography (OC), for the detection of NASBA amplicons of the 18S RNA of Leish- mania was ...the detection of T. ... See full document

6

Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification

... lower detection limit of the assay is 100 – 1000 molecules in vitro RNA for all species, which corresponds to ...79 clinical samples from malaria patients: ...79 samples was confirmed with the ... See full document

6

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates

... equal amplification efficiencies for both targets in the duplex NASBA assay, primer concentrations were further optimized, and half the amount of RNAIII primers was ...All samples were tested in dupli- ... See full document

5

Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets

Subtyping Clinical Specimens of Influenza A Virus by Use of a Simple Method To Amplify RNA Targets

... Alternatively, nucleic acid sequence-based amplification (NASBA) is faster than RT- PCR for RNA amplification and compares favorably with RT-PCR for RNA detection of other ... See full document

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Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens

Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens

... for nucleic acid extraction from different clinical specimens compared to NucliSens miniMAG platform and manual QIAGEN ...real-time nucleic acid sequence-based ... See full document

5

Nucleic Acid Sequence Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR

Nucleic Acid Sequence Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR

... Nucleic acid sequence-based amplification (NASBA) assays were developed for direct detection of Epstein- Barr virus (EBV) transcripts encoding EBV nuclear antigen 1 (EBNA1), ... See full document

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Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season

Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season

... reliable detection of microor- ganisms in clinical specimens by ...the nucleic acid extracts, the number of samples showing inhibition decreased considerably, as also shown previously ... See full document

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Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence Based Amplification

Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence Based Amplification

... failed amplification, since NASBA was invalid on initial testing for 18%, and on final testing for 9%, of ...of amplification by NASBA was most troublesome for throat and NP samples, which resulted ... See full document

5

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp  in Respiratory Specimens

Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens

... isothermal nucleic acid sequence-based amplification (NASBA) was developed to detect Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella ...real-time detection, ... See full document

7

Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification

Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification

... 25°C. Nucleic acids were extracted, and NASBA was performed. Nucleic acid ...extraction. Nucleic acids were extracted as described by Boom et ...a clinical specimen, 100 ␮l of a ... See full document

7

Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay

Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay

... isothermal nucleic acid sequence-based amplification (NASBA) assay was optimized to amplify viral RNA of all four dengue virus serotypes by a set of universal primers and to type the ... See full document

5

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St  Louis Encephalitis Viruses

Nucleic Acid Sequence Based Amplification Assays for Rapid Detection of West Nile and St Louis Encephalitis Viruses

... these samples had low levels of WN viral RNA rather than false-positive NASBA assay ...these samples had low levels of WN viral RNA rather than false-positive ...these samples were depleted and no ... See full document

8

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications

... in clinical use (Rodríguez-Lázaro et ...the detection of viable microorganisms in foods, NASBA will need to be applied directly, ...the samples in nutrient media to increase the number of target ... See full document

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Detection of Four Plasmodium Species by Genus  and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis

... blood samples, without time-consuming and expensive DNA extraction with a commercial kit ...DNA amplification can be detected with the naked eye or a turbi- dimeter ...for amplification can be simply ... See full document

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Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification

Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification

... HPV45. Amplification was performed in a 20-␮l reaction mixture containing 1⫻ REDTaq ReadyMix PCR mix (Sigma- Aldrich, ...promoter sequence was purified using the QIAprep Spin Miniprep kit (Qiagen) according ... See full document

6

Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector

Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector

... of Trypanosoma brucei parasites that cause human sleeping sickness and are partly responsible for animal trypanosomiasis (Nagana disease) in sub-Saharan ...b. brucei proline catabolism for procyclic ... See full document

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Considerations on the use of nucleic acid based amplification for malaria parasite detection

Considerations on the use of nucleic acid based amplification for malaria parasite detection

... parasite detection is best justified only when the potential to detect sub-microscopic infections is achieved, for example identification of individuals with persistent sub-patent infections in the context of ... See full document

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Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola

Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola

... complementary sequence F1 (F1c) and direct sequence F2 ...complementary sequence B1 (B1c) and direct sequence B2 ...(the sequence complementary to B3), are located outside the F2-B2 ... See full document

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Detection of Group 1 Trypanosoma brucei gambiense by Loop Mediated Isothermal Amplification

Detection of Group 1 Trypanosoma brucei gambiense by Loop Mediated Isothermal Amplification

... low detection of parasite DNA in the most used patient sample, blood (samples OM and RPH1 to -4), using the available DNA detection ...LAMP-positive samples (Table ...a based on a ... See full document

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