[PDF] Top 20 Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cells.
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Nucleotide priming and reverse transcriptase activity of hepatitis B virus polymerase expressed in insect cells.
... baculoviruses. Cells were pelleted at 100 3 g for 10 min and washed three times with phosphate-buffered saline ...Bound polymerase was eluted with ...the polymerase open reading ...a ... See full document
9
Mapping of the Hepatitis B Virus Reverse Transcriptase TP and RT Domains by Transcomplementation for Nucleotide Priming and by Protein-Protein Interaction
... titis B virus (DHBV) pol has been expressed by in vitro trans- lation (50) and as an active fusion protein of DHBV Pol in a virus-like particle from the yeast retrotransposon Ty1 ... See full document
9
Protein-Primed Terminal Transferase Activity of Hepatitis B Virus Polymerase
... epatitis B virus (HBV) is an important human pathogen and a member of the family Hepadnaviridae, which comprises a group of pararetroviruses replicating a double-stranded DNA ge- nome through an RNA ... See full document
15
Identification of an Essential Molecular Contact Point on the Duck Hepatitis B Virus Reverse Transcriptase
... LMH cells, and 3 days later intracellular viral core particles were isolated by sedimentation through a sucrose ...endogenous polymerase assay), and then viral nucleic acids were purified and resolved by ... See full document
7
Interaction between Hepatitis B Virus Core Protein and Reverse Transcriptase
... coinfected insect cells with baculoviruses expressing WT core and the HCV protein NS5B that contains a FLAG ...are expressed in mammalian cells, Huh7 cells were coinfected with vaccinia ... See full document
11
Susceptibility of lamivudine resistant hepatitis B virus to other reverse transcriptase inhibitors
... HBV polymerase gene ...the B domain of the polymerase gene associated with the M552V mutant ...Because reverse transcriptase has a fundamental role in the replication of retroviruses ... See full document
7
Cryptic Protein Priming Sites in Two Different Domains of Duck Hepatitis B Virus Reverse Transcriptase for Initiating DNA Synthesis In Vitro
... protein priming (45, 49). The possibility that authentic site priming depends on priming first at the cryptic sites, which would be unprecedented for protein-primed DNA or RNA synthesis, has been ... See full document
12
Reconstitution of a Functional Duck Hepatitis B Virus Replication Initiation Complex from Separate Reverse Transcriptase Domains Expressed in Escherichia coli
... Plasmid constructs. The parental plasmid for in vitro translations was pT7AMVpol16 (41), which contains the T7 promoter followed by nt 170 to 2843 of DHBV type 16, i.e., the complete P protein open reading frame (ORF) ... See full document
10
Comparative Analysis of Hepatitis B Virus Polymerase Sequences Required for Viral RNA Binding, RNA Packaging, and Protein Priming
... tein priming (i.e., transferase) activity of the Y147A mutant, in contrast to its severe defect in Mg 2⫹ -dependent and H ε -templated protein priming, indicates that it is possible to separate ... See full document
9
A Single Amino Acid in the Reverse Transcriptase Domain of Hepatitis B Virus Affects Virus Replication Efficiency
... the hepatitis B virus (HBV) polymerase, two naturally occurring HBV isolates (56 and 2-18) with ...HepG2 cells, HBV DNA isolated from intracellular virus core particles was much ... See full document
7
Generation of Replication-Competent Hepatitis B Virus Nucleocapsids in Insect Cells
... This priming step yields a discrete 3- to 4-deoxyribonucleotide oligomer which is covalently linked to Pol, the result of a unique priming mechanism (49) wherein deoxyribonucleoside triphosphates (dNTPs) ... See full document
12
Hepatitis B Virus Reverse Transcriptase and ε RNA Sequences Required for Specific Interaction In Vitro
... enzymatic activity), were dispensable for ε bind- ...an insect cell expression system and displayed low levels of protein-priming activity in vitro, although this low priming ... See full document
10
Inhibition of Hepatitis B Virus Polymerase by Entecavir
... with activity against hepatitis B virus ...nucleoside/tide reverse transcriptase inhibitors approved for HBV therapy, lamiv- udine (LVD) and adefovir (ADV), in several ways: ETV ... See full document
10
Requirement of Heat Shock Protein 90 for Human Hepatitis B Virus Reverse Transcriptase Function
... protein priming activity of the HBV RT con- trasts sharply with the efficient protein priming activity ob- tained with similar DHBV mini-RT proteins using the same Hsp90 reconstitution ... See full document
10
In Vitro Reconstitution of a Functional Duck Hepatitis B Virus Reverse Transcriptase: Posttranslational Activation by Hsp90
... host cells; efforts to extract an active RT from either viral particles or infected cells for in vitro analysis have been unsuccessful (2, ...duck hepatitis B virus (DHBV) RT. The DHBV ... See full document
9
Transcomplementation of nucleotide priming and reverse transcription between independently expressed TP and RT domains of the hepatitis B virus reverse transcriptase.
... The hepatitis B virus (HBV) reverse transcriptase, designated pol, is composed of four ...of reverse transcription; (ii) the spacer domain, which is tolerant of mutations; (iii) ... See full document
9
In Vitro Epsilon RNA-Dependent Protein Priming Activity of Human Hepatitis B Virus Polymerase
... human cells, pCMV-HE was produced by substituting a ...RNA polymerase II (Pol II) tran- scription in transfected mammalian cells, a capped and polyadenylated HBV RNA initiating at the authentic pgRNA ... See full document
18
The Majority of Duck Hepatitis B Virus Reverse Transcriptase in Cells Is Nonencapsidated and Is Bound to a Cytoplasmic Structure
... the polymerase for immunoprecipi- ...the polymerase when it was expressed in the ab- sence of the core protein (data not shown), eliminating the possibility that polymerase detected by ... See full document
10
RNase H domain of Moloney murine leukemia virus reverse transcriptase retains activity but requires the polymerase domain for specificity.
... retained activity and increasing amounts of this preparation failed to reveal any contaminating ...H activity than GST-RNH, even after correction for the relative molar amounts of the two proteins present ... See full document
9
Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase.
... Recently, we demonstrated that the polymerase of duck hepatitis B virus DHBV synthesized in an in vitro translation reaction exhibits reverse transcriptase activity and that the viral po[r] ... See full document
8
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