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[PDF] Top 20 PCR-based assay for Mycoplasma hyopneumoniae

Has 10000 "PCR-based assay for Mycoplasma hyopneumoniae" found on our website. Below are the top 20 most common "PCR-based assay for Mycoplasma hyopneumoniae".

PCR based assay for Mycoplasma hyopneumoniae

PCR based assay for Mycoplasma hyopneumoniae

... it also con tains a probe that hybridizes to the target nucleic acid sequence ampli?ed by the use of the primer pair of the invention in the polymerase chain reaction.. the [r] ... See full document

14

Detection of Mycoplasma hyopneumoniae in Bronchoalveolar Lavage Fluids of Pigs by PCR

Detection of Mycoplasma hyopneumoniae in Bronchoalveolar Lavage Fluids of Pigs by PCR

... M. hyopneumoniae in pigs with chronic pneumonia obtained by cultivation, immunofluores- cence tests, and PCR (i) with BALFs incubated in Friis me- dium, (ii) with DNA extracted from BALFs, and (iii) with ... See full document

5

Diagnosis and Differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis Infections in Pigs by PCR Amplification of the p36 and p46 Genes

Diagnosis and Differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis Infections in Pigs by PCR Amplification of the p36 and p46 Genes

... permit PCR amplification of entire p36 and p46 genes and of internal fragments of these ...other mycoplasma species, bacteria, and viruses commonly associated with respiratory diseases in ... See full document

7

Real Time PCR Assays To Address Genetic Diversity among Strains of Mycoplasma hyopneumoniae

Real Time PCR Assays To Address Genetic Diversity among Strains of Mycoplasma hyopneumoniae

... 2008 Mycoplasma hyopneumoniae is an important cause of pneumonia in pigs around the world, but confirm- ing its presence in (or absence from) pigs can be ...Numerous PCR assays for the detection of ... See full document

8

Selective medium for culture of Mycoplasma hyopneumoniae

Selective medium for culture of Mycoplasma hyopneumoniae

... 2.1. Mycoplasma strains M. hyopneumoniae strains 277/94 and 325/95 were the gift of Niels Friis, Danish Veterinary Institute, ...M. hyopneumoniae were Danish fi eld isolates from lesions of EP, the ... See full document

7

Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium

Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium

... 2005 Mycoplasma genitalium is known to cause nonchlamydial, nongonococcal urethritis in men and to be asso- ciated with pelvic inflammatory disease in ...sensitive PCR methods are needed for diagnosis of ... See full document

8

Survey on Mycoplasma hyopneumoniae gilt acclimation practices in Europe

Survey on Mycoplasma hyopneumoniae gilt acclimation practices in Europe

... M. hyopneumoniae vaccination (145 out of 249, ...M. hyopneumoniae initial status and performing acclimation strategies against it, verified the effect of the acclimation by ELISA (22 out of 53, ...41.5%), ... See full document

8

Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae

... Abstract Mycoplasma hyopneumoniae is a prevalent swine respiratory pathogen that is a major cause of economic loss to pig ...M. hyopneumoniae and in developing new vaccines is the lack of tools to ... See full document

10

Genetic diversity of Mycoplasma hyopneumoniae isolates of abattoir pigs

Genetic diversity of Mycoplasma hyopneumoniae isolates of abattoir pigs

... and PCR-RFLP should be considered as reliable tests for quick and rather inexpensive differentiation of ...M. hyopneumoniae strains without prior ...M. hyopneumoniae and this was not achieved with ... See full document

9

MLVA typing of Mycoplasma hyopneumoniae bacterins and field strains

MLVA typing of Mycoplasma hyopneumoniae bacterins and field strains

... developed PCR assays could have suf fi cient sensitivity for M hyopneumoniae typing from clinical samples, a com- plete characterisation by MLVA is not always possible using conventional PCR, even ... See full document

6

Development of a Genomics Based PCR Assay for Detection of Mycoplasma pneumoniae in a Large Outbreak  in New York State

Development of a Genomics Based PCR Assay for Detection of Mycoplasma pneumoniae in a Large Outbreak in New York State

... genomics-based PCR method was developed and used to test specimens from patients involved in a large outbreak of Mycoplasma pneumoniae in a closed religious community in New York ...This PCR ... See full document

6

Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae

Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae

... Slovakia) was used as the positive control for the PCR detection of Mycoplasma hyopneumoniae. Cultivation To proof Pasteurella and Actinobacillus species the cultivation method on the blood agar, ... See full document

7

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

... Abstract Mycoplasma hyopneumoniae, the primary pathogenic bacterium causing enzootic pneumonia, significantly affects worldwide swine ...M. hyopneumoniae are desirable, especially in early ...M. ... See full document

10

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

Serum metabolite markers of early Mycoplasma hyopneumoniae infection in pigs

... Mycoplasma hyopneumoniae infection in pigs Meera Surendran Nair 1 , Dan Yao 2 , Chi Chen 2 and Maria Pieters 1* Abstract Mycoplasma hyopneumoniae, the primary pathogenic bacterium causing ... See full document

11

A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae

... Li Huang 1,2 , Zhixun Xie 1* , Liji Xie 1 , Xianwen Deng 1 , Zhiqin Xie 1 , Sisi Luo 1 , Jiaoling Huang 1 , Tingting Zeng 1 and Jiaxun Feng 2 Abstract Background: Infectious arthritis in broilers represents an economic ... See full document

9

Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

Transposon mutagenesis in Mycoplasma hyopneumoniae using a novel mariner-based system for generating random mutations

... Linker PCR was repeated for each individual transformant, and agarose gel electrophoresis showed that the amplicons gen- erated were identical in size to those obtained prior to serial ...of PCR amplicons ... See full document

11

Impact of diversity of Mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs

Impact of diversity of Mycoplasma hyopneumoniae strains on lung lesions in slaughter pigs

... M. hyopneumoniae was detected in the scalding water, the lungs of SPF pigs remained nega- tive by nested PCR ...M. hyopneumoniae strains were found, illustrat- ing for the first time the importance ... See full document

14

Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay

Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay

... hyorhinis and on a collection of bacterial strains phylogenetically close to M. hyorhinis. MLST has the added advantages of providing meaningful information about population structure. The MLST work here demonstrates ... See full document

8

A comparison study between GeXP based multiplex PCR and serology assay for Mycoplasma pneumoniae detection in children with community acquired pneumonia

A comparison study between GeXP based multiplex PCR and serology assay for Mycoplasma pneumoniae detection in children with community acquired pneumonia

... laboratory diagnosis methods of M. pneumoniae infec- tion for childhood CAP in larger clinical database has not been reported. In the present study, we applied a multiplex-PCR, named GeXP assay, to detected ... See full document

7

Rapid Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum Organisms in Genitourinary Samples by PCR Microtiter Plate Hybridization Assay

Rapid Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum Organisms in Genitourinary Samples by PCR Microtiter Plate Hybridization Assay

... of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms, which are thought to be associated with nongonococcal urethritis (NGU) and other genitourinary ... See full document

6

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