[PDF] Top 20 Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV 1) Group M Subtypes, Group O, and HIV 2
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Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV 1) Group M Subtypes, Group O, and HIV 2
... the HIV-1/2 qual- itative RNA assay: specific primers for amplification of the target, a specific probe to which the amplified target can hy- bridize, and specific immunoassay reagents for ... See full document
8
Rapid Assay for Simultaneous Detection and Differentiation of Immunoglobulin G Antibodies to Human Immunodeficiency Virus Type 1 (HIV 1) Group M, HIV 1 Group O, and HIV 2
... discriminates human immunodeficiency virus (HIV) infec- tions on the basis of viral type, HIV type 1 (HIV-1) group M, ... See full document
5
Seven Human Immunodeficiency Virus (HIV) Antigen Antibody Combination Assays: Evaluation of HIV Seroconversion Sensitivity and Subtype Detection
... the performance of two prototype human immunodeficiency virus (HIV) antigen- antibody (Ag-Ab) combination assays, one from Abbott Laboratories (AxSYM HIV Ag-Ab) and the other ... See full document
7
Prevalence of Human Immunodeficiency Virus Type 1 (HIV 1) Non B Subtypes in Foreigners Living in Madrid, Spain, and Comparison of the Performances of the AMPLICOR HIV 1 MONITOR Version 1 0 and the New Automated Version 1 5
... different HIV-1 subtypes in a single geo- graphic region coupled with intersubtype recombination (16, 26) has serious implications for the efforts to control the AIDS ...genetic subtypes on ... See full document
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Simple Subtyping Assay for Human Immunodeficiency Virus Type 1 Subtypes B, C, CRF01 AE, CRF07 BC, and CRF08 BC
... a human immunodeficiency virus type 1 (HIV-1) subtype-screening tool by nested multiplex PCR, we further improve it through the redesign of subtype-specific ... See full document
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Comparative Performance of Three Viral Load Assays on Human Immunodeficiency Virus Type 1 (HIV 1) Isolates Representing Group M (Subtypes A to G) and Group O: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR Version 1 5, and Quantiplex HIV 1 RNA Version 3 0
... of human immunodeficiency virus type 1 (HIV-1) infection and implementation of treatment strate- gies rely on the quantitative measurement of HIV-1 RNA in ... See full document
9
Impact of Human Immunodeficiency Virus Type 1 (HIV 1) Genetic Diversity on Performance of Four Commercial Viral Load Assays: LCx HIV RNA Quantitative, AMPLICOR HIV 1 MONITOR v1 5, VERSANT HIV 1 RNA 3 0, and NucliSens HIV 1 QT
... Human immunodeficiency virus type 1 (HIV-1) evolution and changing strain distribution present a chal- lenge to nucleic acid-based ...the detection and accurate ... See full document
9
Frequency, Causes, and New Challenges of Indeterminate Results in Western Blot Confirmatory Testing for Antibodies to Human Immunodeficiency Virus
... antigen assay is restricted to detecting infections in newborns of HIV-positive mothers or individuals who have had very recent high-risk exposure ...earlier detection, nucleic acid tests were often ... See full document
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Detection of Phylogenetically Diverse Human Immunodeficiency Virus Type 1 Groups M and O from Plasma by Using Highly Sensitive and Specific Generic Primers
... of HIV-1 transmission by blood transfusion with blood screened with the current anti- body and antigen tests is estimated to be two donations per million units of blood donated ...of HIV-1, ... See full document
6
PCR Based Assay To Quantify Human Immunodeficiency Virus Type 1 DNA in Peripheral Blood Mononuclear Cells
... of human immunodeficiency virus type 1 (HIV-1) RNA in plasma is a powerful prognostic tool in clin- ical practice ...50-copy/ml detection limit of the AMPLICOR ... See full document
5
Analysis of Genetic Variability within the Immunodominant Epitopes of Envelope gp41 from Human Immunodeficiency Virus Type 1 (HIV 1) Group M and Its Impact on HIV 1 Antibody Detection
... an assay based on a conserved sequence within the gp41 region which is highly sensitive for amplification of viral RNA from plasma of HIV-1-positive specimens repre- senting different subtypes ... See full document
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Development of Calibrated Viral Load Standards for Group M Subtypes of Human Immunodeficiency Virus Type 1 and Performance of an Improved AMPLICOR HIV 1 MONITOR Test with Isolates of Diverse Subtypes
... of HIV-1 RNA concentration has been ...the 2- to 3-log difference that Nolte et ...single HIV-1 stock calibrated by cosedimenta- tion of latex spheres and virions which were ... See full document
7
Simultaneous Detection of Multiplex Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere Based Hybridization Assay
... groups: group 1, HCV infection only (n ⫽ 13); group 2, HIV-1 infection only (n ⫽ 8); group 3, concurrent HIV-1 and HCV infection (n ⫽ 12); group 4, ... See full document
6
Performance of a Novel Human Immunodeficiency Virus (HIV) Type 1 Total Nucleic Acid Based Real Time PCR Assay Using Whole Blood and Dried Blood Spots for Diagnosis of HIV in Infants
... pediatric HIV-1 infection in resource-limited settings (18, ...the HIV-1 secondary standard showed an excellent LOD of 1,090 copies/ml with an equiva- lent input of 60 l of whole blood, ... See full document
5
Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... the LCx HIV RNA Quantitative ...the assay controls and clinical samples were ex- tracted by centrifugation and separation using a QIAAmp col- umn and subjected to amplification with primers targeted ... See full document
5
Flow cytometric immunofluorescence assay for detection of antibodies to human immunodeficiency virus type 1 using insoluble precursor forms of recombinant polyproteins as carriers and antigens
... serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 ... See full document
8
Mechanistic differences between HIV 1 and SIV nucleocapsid proteins and cross species HIV 1 genomic RNA recognition
... The HIV-1 Gag protein lacking the p6 domain (HIV-1 Gag∆p6, also referred to simply as “Gag”) was purified as previously described [27, ...11,560 M −1 cm −1 for ... See full document
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Sensitivity and Specificity of Human Immunodeficiency Virus Rapid Serologic Assays and Testing Algorithms in an Antenatal Clinic in Abidjan, Ivory Coast
... to human immunodeficiency virus (HIV) is important in controlling the impact of the HIV and AIDS epidemic because it allows for the diagnosis and counseling of HIV-infected ... See full document
5
Quantification of low levels of human immunodeficiency virus (HIV) type 1 RNA in P24 antigen negative, asymptomatic, HIV positive patients by PCR
... nested PCR was used to quantify small numbers of human immunodeficiency virus (HIV) type 1 (HIV-1) RNA particles in the serum specimens of 26 p24 ... See full document
5
Use of Real-Time PCR and Molecular Beacons To Detect Virus Replication in Human Immunodeficiency Virus Type 1-Infected Individuals on Prolonged Effective Antiretroviral Therapy
... Amplicor HIV-1 Monitor assay (lower limit of detection, 50 HIV-1 RNA copies/ml; Roche Molecular Systems, Branchburg, ...detectable virus over the duration of follow-up; PS ... See full document
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