[PDF] Top 20 Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein

... HSV-induced phosphorylation of OBP does not affect its ability to bind to OriS site ...that phosphorylation has been shown to regulate the origin binding activity of other viral replication ... See full document

... of herpes simplex virus type 1 ( HSV-1), located between envelope and capsid, contains some important proteins of the ...the protein transports into the ...the ... See full document

... The resulting mixture was subjected to centrifugation for 60 min at 100,000 x g, and the supernatant was studied by Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Lysa[r] ... See full document

... Time courses of HSV-1 receptor binding and internalization compared with effect of neomycin addition at various times postinfection.. Receptor-bound virus at various times postinfection [r] ... See full document

... 3 Thermolabile In Vivo DNA-Binding Activity Associated with a Protein Encoded by Mutants of Herpes Simplex Virus Type 1 Received 30 December 1982/Accepted 17 March 1983 The major DNA-bin[r] ... See full document

... To characterize more thoroughly the kinetics with which the 65KDBP gene is expressed, we have examined not only the accumulation of 6SKDBP mRNA and protein, as compared to other well-cha[r] ... See full document

... The regions of the herpes simplex virus type 1 immediate early protein Vmw 175 required for site specific DNA binding closely correspond to those involved in transcriptional regulation..[r] ... See full document

... UL17-stop virus contains a 16-bp insertion at position 32718 in the HSV-1 strain 17 genome, with stop codons in all three reading frames, truncating the UL17 open reading frame after codon 260 ...with ... See full document

... When comparing unwinding of substrates with 5 ⬘ overhangs (Fig. 4C) to those with 3 ⬘ overhangs (Fig. 4D), we observed that substrates with 5 ⬘ overhangs are preferred over substrates with 3 ⬘ overhangs. These results ... See full document

... viral protein or it is a cellular protein whose expression or modification was induced by the viral ...ICP22 protein was of particular interest since viruses which contain deletions of, and ... See full document

... The disappearance of the 5.4- to 5.7-Mdal EcoRI dDNA fragments after digestion with BamHI and the appearance of a new cluster of fragments, 2.6 to 3.2 Mdal, plus two 1-Mdal fragments B2 [r] ... See full document

... The pooled individual peaks were 383 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have isolated a new cyclic AMP-independent protein kinase activity induced in He[r] ... See full document

... We report herein the genetic and phenotypic characterization of four temperature-sensitive mutants of herpes simplex virus type 1 tsY46, tsE5, tsE6, and tsLG4 that have begun to elucidat[r] ... See full document

... by phosphorylation (6). With the UL13 mutant virus, the release of VP22 was significantly ...the protein were absent, indi- cating that the VP22 modification was also ...mutant virus (3) ... See full document

... To investigate the genetic requirements for the phosphory- lation of Sp1, the modification state of Sp1 was studied as a function of infection with several IE mutants (d120, d109, d106, and 5dl1.2). These viruses possess ... See full document

... fusion protein and its incorporation into the virus ...the virus replication cycle ...fusion protein localized to distinct regions within the nucleus of the infected ...a virus genome ... See full document

... a 1:500 dilution in TBS-T containing 5% milk and allowed to incubate for 1 h at room ...a 1:10,000 dilution in block for 1 h at room ... See full document

... FIG. 1. Genes, proteins, and landmarks of herpesvirus proteinases and substrates. All of the genes shown were expressed using the plasmid pRSV.neo (see Materials and Methods). The primary translation products from ... See full document

... associated protein leads to a decreased ability to bind to mem- branes and/or target to the correct compartment (for example, see reference ...the protein is palmitylated and stably an- ... See full document

... Membrane flotation gradient centrifugation. Methods for analysis of mem- brane-associated proteins by sucrose density gradient flotation have been de- scribed previously (30, 42, 55). By using a modified form of this ... See full document