[PDF] Top 20 ICP27 Phosphorylation Site Mutants Are Defective in Herpes Simplex Virus 1 Replication and Gene Expression
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ICP27 Phosphorylation Site Mutants Are Defective in Herpes Simplex Virus 1 Replication and Gene Expression
... Herpes simplex virus 1 (HSV-1) protein ICP27 is a multifunctional regulatory protein that is posttransla- tionally modified by phosphorylation during viral ...infection. ... See full document
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ICP27 Phosphorylation Site Mutants Display Altered Functional Interactions with Cellular Export Factors Aly/REF and TAP/NXF1 but Are Able To Bind Herpes Simplex Virus 1 RNA
... Herpes simplex virus 1 (HSV-1) protein ICP27 is a multifunctional regulatory protein that is phosphory- ...lated. Phosphorylation can affect protein localization, protein ... See full document
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Phosphorylation Site Mutations Affect Herpes Simplex Virus Type 1 ICP0 Function
... With site-directed mutagenesis, potentially phosphorylated serine and threonine residues in these regions were changed to ala- ...(Phos 1 and 3) in Vero ...Phos 1 form of ICP0 exhibited significantly ... See full document
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Processing of α-Globin and ICP0 mRNA in Cells Infected with Herpes Simplex Virus Type 1 ICP27 Mutants
... of ICP27. The observation that certain mutant forms of ICP27 cause a significant change in the heterogeneity in length of the ␣-glo- bin mRNA poly(A) tail was surprising and points to a previ- ously ... See full document
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Prolonged gene expression and cell survival after infection by a herpes simplex virus mutant defective in the immediate-early genes encoding ICP4, ICP27, and ICP22.
... infection, herpes simplex virus (HSV) expresses four immediate-early (IE) regulatory proteins, ICP4, ICP0, ICP22, and ...resulting mutants, should provide insights into how these proteins ... See full document
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Identification of an ICP27-Responsive Element in the Coding Region of a Herpes Simplex Virus Type 1 Late Gene
... with ICP27 expression plasmid pC27 (27), except when the plasmid pM27 (34) was used to express ICP27 (see ...poly(A) site replaced by that from the simian virus 40 (SV40) late poly(A) ... See full document
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Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes.
... taining 1% FCS, as indicated in the ...Polyadenylation site consensus sequences are marked with downward arrows and the nucleotide ...d27 virus genome is indicated immediately below the genome scale ... See full document
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Herpes Simplex Virus Type 1 ICP27-Dependent Activation of NF-κB
... intron 1 and exons 2 and 3 of ICP0, as well as upstream of the late UL46 and UL47 genes ...HSV-induced expression of cellular genes regulated by NF- B have been ...HSV-1, expression of ... See full document
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Roles of p53 in Herpes Simplex Virus 1 Replication
... promoted ICP27 expression in early infection in an ICP22-independent ...manner. ICP27 plays essential roles in HSV-1 replication by regulating all stages of mRNA biogenesis, including ... See full document
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Function of the Herpes Simplex Virus 1 Small Capsid Protein VP26 Is Regulated by Phosphorylation at a Specific Site
... FIG 1 (A) Schematic diagrams of the genome structure of the wild-type and recombinant HSV-1 viruses used in this ...Line 1, the wild-type HSV-1(F) genome; line 2, the wild-type ... See full document
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Herpes Simplex Virus ICP27 Is Required for Virus-Induced Stabilization of the ARE-Containing IEX-1 mRNA Encoded by the Human IER3 Gene
... HSV gene products have been unsuccessful, as IEX-1 mRNA appears to be stabilized by the stresses associated with the transfection protocol (data not ...that ICP27 is directly responsible for the ... See full document
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Cellular SNF2H Chromatin Remodeling Factor Promotes Herpes Simplex Virus 1 Immediate Early Gene Expression and Replication
... for ICP27, 5=-CCGCCGGCC TGGATGTGACG-3= [forward] and 5=-CGTGGTGGCCGGGGTGGTG CTC-3= [reverse]; for the GAPDH pseudogene, 5=-TTCGACAGTCAGC CGCATCTTCTT-3= [forward] and 5=-CAGGCGCCCAATACGACCAA ATC-3= [reverse]; for ... See full document
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The UL12 Protein of Herpes Simplex Virus 1 Is Regulated by Tyrosine Phosphorylation
... UL12 gene encodes both full-length UL12 and ...baculovirus expression system, ...(Fig. 1), this Tyr residue may also be phosphorylated in ...this phosphorylation in HSV-1- infected ... See full document
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Phenotypic Suppression of a Herpes Simplex Virus 1 ICP27 Mutation by Enhanced Transcription of the Mutant Gene
... functional gene product can suppress a mutant phenotype ...dLeu ICP27 gene, reasoning that such lines might complement dLeu by providing an additional source of dLeu ...dLeu ICP27 gene ... See full document
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Improved cell survival by the reduction of immediate-early gene expression in replication-defective mutants of herpes simplex virus type 1 but not by mutation of the virion host shutoff function.
... 10 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Improved Cell Survival by the Reduction of Immediate-Early Gene Expression in Replication-Defective Mutants o[r] ... See full document
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Herpes Simplex Virus 1 ICP0 Phosphorylation Site Mutants Are Attenuated for Viral Replication and Impaired for Explant-Induced Reactivation
... Phos 1, its mutation lies in the proline-rich transactiva- tion domain and is adjacent to the RING-finger domain of ICP0 ...(Fig. 1). We have previously shown that Phos 1 is im- paired for ICP0’s E3 ... See full document
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Roles of the Phosphorylation of Herpes Simplex Virus 1 UL51 at a Specific Site in Viral Replication and Pathogenicity
... its phosphorylation at the identified phosphorylation sites, we constructed a series of UL51 mutant viruses using a self-excisable HSV-1(F) bacterial artificial chromosome (BAC) clone (pYEbac102Cre) ... See full document
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Analysis of the Phosphorylation Sites of Herpes Simplex Virus Type 1 Regulatory Protein ICP27
... mutant ICP27 constructs, as indicated in Table 1, or with wild-type ICP27 or pGEM-1 vector ...an ICP27 deletion virus, ...(Table 1). In this assay, pGEM-1 served as ... See full document
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Cytotoxicity of a replication-defective mutant of herpes simplex virus type 1.
... It is also possible that early gene expression in cells infected by HSV-1 strains which have an intact IE 3 gene contributes to the induction of CPE, particularly since IE 3-expressing s[r] ... See full document
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The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation
... temporal expression of UL24 tran- scripts was regulated by differential polyadenylation ...poly(A) site, are expressed maximally at early times in infection and then de- crease, whereas long transcripts ... See full document
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