[PDF] Top 20 The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells
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The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells
... The herpes simplex virus (HSV) UL16 gene is conserved throughout the Herpesviridae and encodes a poorly understood tegument ...HSV-1 UL16 protein forms complexes with several ... See full document
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Elucidation of the Block to Herpes Simplex Virus Egress in the Absence of Tegument Protein UL16 Reveals a Novel Interaction with VP22
... insight, cells infected with UL16-null mutants were examined by electron ...of capsids to cytoplasmic membranes were observed, but the wrapping of capsids with membranes was de- ... See full document
10
Nuclear Egress and Envelopment of Herpes Simplex Virus Capsids Analyzed with Dual-Color Fluorescence HSV1(17+)
... Unbound virus was removed by extensive washing, and the cells were transferred to 37°C and fixed at 4, 6, 8, 10, or 12 h ...in infected cells at different times ...of cells ... See full document
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A Null Mutation in the UL36 Gene of Herpes Simplex Virus Type 1 Results in Accumulation of Unenveloped DNA-Filled Capsids in the Cytoplasm of Infected Cells
... these capsids to a cytoplasmic maturation compartment for final ...a critical maturation point for the capsids as they traverse to the cell ...C capsids produced are not transported to this ... See full document
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Identification and Characterization of the UL56 Gene Product of Herpes Simplex Virus Type 2
... UL56 gene product of herpes simplex virus (HSV) has been shown to play an important role in viral ...type 2 (HSV-2) and examined its expression and properties. The ... See full document
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Herpes simplex virus glycoprotein K promotes egress of virus particles.
... for virus replication, F-gK b was propagated on complementing cells which can express ...nonfused cells when plated on complementing cells, although syncytia were observed when the ... See full document
13
Comparative Analysis of UL16 Mutants Derived from Multiple Strains of Herpes Simplex Virus 2 (HSV-2) and HSV-1 Reveals Species-Specific Requirements for the UL16 Protein
... the herpes simplex virus (HSV) UL16 gene are conserved throughout the ...a UL16-null mutant derived from HSV-2 strain 186 revealed a roughly 100-fold replica- tion ... See full document
15
The Us2 Gene Product of Herpes Simplex Virus 2 Is a Membrane-Associated Ubiquitin-Interacting Protein
... 293T cells. At 24 h posttransfection, cells were col- lected, and membranes were purified by membrane flotation as described above for ...cised from SDS-PAGE gels and were identified by LC–MS-MS FIG ... See full document
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The null mutant of the U(L)31 gene of herpes simplex virus 1: construction and phenotype in infected cells.
... construction from an appropriate cosmid library a deletion mutant which replicates in rabbit skin cells carrying the U L 31 gene under a late ( g 1 ) viral ...mutant virus exhibits cytopathic ... See full document
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Origin of unenveloped capsids in the cytoplasm of cells infected with herpes simplex virus 1.
... blocking fusion of the envelope with cell membranes; it is conceivable that the unenveloped capsids present in the cytoplasm of cells infected with wild-type virus reflect occasional fus[r] ... See full document
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The UL11 gene of herpes simplex virus 1 encodes a function that facilitates nucleocapsid envelopment and egress from cells.
... obtained from the medium and cell lysates of cells infected with the wild-type and restored viruses were virtually identical, we conclude that the decreased production of infectious viru[r] ... See full document
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Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface, and the egress of virions from infected cells.
... (0) or treated with Electron microscopy of HSV-1-infected HEp-2 I with [35S]methio- cells. As monensin severely inhibited the appear- HSV-1, and then ance of HSV-1 glycoproteins at infec[r] ... See full document
11
Characterization of VP22 in Herpes Simplex Virus-Infected Cells
... Kingdom 2 Received 11 April 2005/Accepted 8 July 2005 We examine biochemical characteristics of the herpes simplex virus (HSV) tegument protein VP22 by gel filtration, glycerol sedimentation, ... See full document
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Regulated Interaction of Tegument Proteins UL16 and UL11 from Herpes Simplex Virus
... In experiments described below, we addressed the hypothesis that the UL11 binding in UL16 also occurs within the first 155 residues of UL16. This was found to be the case, and the binding site was mapped to ... See full document
13
Herpes simplex virus capsids assembled in insect cells infected with recombinant baculoviruses: structural authenticity and localization of VP26.
... six herpes simplex virus type 1 genes results in the formation of capsid-like ...comparing capsids assembled with and without the expression of gene UL35, we have confirmed the presence ... See full document
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In vitro synthesis of DNA in nuclei isolated from human lung cells infected with herpes simplex type II virus.
... The rate of DNA synthesis for the in vitro-isolated nuclei system was assayed by incubating nuclei isolated at the indicated times for HEL cells infected with herpesvirus, as described a[r] ... See full document
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Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.
... Ribonucleic acid synthesis in cells infected with herpes simplex virus: characterization of viral high molecular weight nuclear RNA. RNA synthesis[r] ... See full document
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Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.
... The cells were labeled from 5 to 18 h after infection with [3H]thymidine, and then extraction, sucrose gradient purification, sample deproteinization, and CsCl centrifugation were perfor[r] ... See full document
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Ribonucleic Acid Synthesis in Cells Infected with Herpes Simplex Virus
... cytoplasmic RNA specified by the virus, 2 X 108 HEp-2 cells were infected with a multiplicity of 50 PFU per cell and were labeled with 3H-uridine. (10 Ac/ml) for 1 hr at 4.5 hr after inf[r] ... See full document
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Chromosomal and Autoradiographic Studies of Cells Infected with Herpes Simplex Virus
... In order to determine whether the decline in the capacity of irradiated virus to induce chromo- somal aberrations was related to a decrease in the adsorption of virus, BHK-21 cells were [r] ... See full document
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