[PDF] Top 20 EBP2, a Human Protein That Interacts with Sequences of the Epstein-Barr Virus Nuclear Antigen 1 Important for Plasmid Maintenance

... stable maintenance of latent Epstein-Barr virus (EBV) DNA episomes in human cells requires only one viral protein, Epstein-Barr nuclear antigen ... See full document

... these sequences in the Brd4-mediated segregation ...in human cells, where EBNA1 ⌬ 325–376 bound EBNA1 to the same degree as did wild-type EBNA1, but no binding was detected with EBNA1 ⌬ ...in human ... See full document

... Epstein-Barr virus (EBV) is present in tumor cells of more than 90% of cases of Burkitt’s lymphoma (BL) in areas of endemicity in equatorial Africa and New Guinea, as well as in tumor cells of about ... See full document

... How important each of these potential immune escape mechanisms is to the phenomenon of EBV persistence remains a matter of ...the plasmid maintenance-transcriptional transactivation proper- ties of ... See full document

... N-terminal sequences spanning S21 disrupted the ability of EBNA1 to function in EBV replication, plasmid maintenance or tran- scriptional activation (30, 45; our unpublished data), nor is the S21 ... See full document

... with protein A-Sepharose beads incubated with lysates of transfected cells harvested 48 h after ...expression plasmid and not in lanes without EBNA1 transfected ...of protein loaded were equivalent ... See full document

... EBNA-3C interacts with both RBP-J k and an activating factor but that only one protein can be bound at a time or only one complex can interact with DNA because of adjacent binding sites in the ...as ... See full document

... the virus genome maintenance protein ...EBNA1 protein is composed of unique N-terminal (residues 1 to 89) and C-ter- minal (residues 328 to 641) domains flanking a large Gly-Ala repeat, ... See full document

... DNA-binding protein CBF1 (also known as recombination signal-binding protein [RBP]-J k ...corepressor interacts with EBNA3C in this DNA binding ...expression plasmid for human histone ... See full document

... this antigen in both eukaryotic and prokaryotic expres- sion ...[Fig. 1]) lacking the Gly-Ala repeat was constructed by PCR deletional mutagenesis as described elsewhere ...bound protein was then ... See full document

... Table 1, as are the TaqMan probes and GAPDH primers purchased from Applied Biosystems (GAPDH Control Reagents, ABI ...from 1 ␮g total RNA with 2 pmol RT primer in a 20- ␮ l reaction mixture with SuperScript ... See full document

... the human influenza HA sequence (HA-43GAr-Ova) ...fused protein and several amino acids in front of the AUG codon, which corresponds more to its nat- ural position in N-terminal part of EBNA-1 ... See full document

... reporter plasmid containing an HSV TK TATA box and a 71-bp LMP-1 promoter fragment extending from bp ⫺215 to ⫺144 (indicated on the left) were mutated entirely (mIRE-distal and mIRE-proximal, absence of ... See full document

... a protein that is not demonstrably cross-reactive with Epstein-Barr virus nuclear antigen-1 or with any other EBV protein, but forms complexes with other ...viral ... See full document

... Fp-directed expression of hGH was used as an indicator of promoter activity in type I A and type III B latently infected Mutu BL cells and in EBV-negative Louckes BL cells C following tr[r] ... See full document

... In lanes 1 to 11, in vitro kinase reactions with LMP2, P-Tyr, or control antibody immunoprecipitates from BJAB cells transfected with pSG5, pLMP2A, or LMP2A deletion mutants described in[r] ... See full document

... When Raji cells are allowed to grow to high density and most of the cells become growth arrested in the G, phase of the cell cycle, the level of detectable latent membrane protein 1 LMP1[r] ... See full document

... Thus, the phenotype of the deletion of the last 45 amino acids of EBNA-LP was profoundly evident when primary B lymphocytes were infected in an endpoint dilution clonal transformation as[r] ... See full document

... The objectives of the experiments described here were i to construct a recombinant eucaryotic vector for the reproducible expression of EBNA2 in a wide variety of cells, ii to determine [r] ... See full document

... Presence and relative amount of the 53K component in different cell types Relative amt of 53K' EBNA Origin Cell Undetectable Normal B-lymphocytes Undetectable Protein A-stimulated Blymph[r] ... See full document