[PDF] Top 20 Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia
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Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia
... In the present investigation, a cell free system for protein synthesis has been developed from human reticulocytes and this latter hypothesis tested further by comparing the capacity for[r] ... See full document
8
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments
... parasite protein interactions with their target cells (reticulocytes) has been studied with molecules obtained from eukaryote ...germ cell-free sys- tem [22]) or prokaryote (mainly ...coli ... See full document
9
Synthetic Biology Goes Cell-Free
... of protein–protein interactions and the resulting complexes that these interactions can ...at synthesis of such ...the cell-free expression of hepatitis B core antigen VLP (2 subunits) ... See full document
14
Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia
... beta thalassemia and sickle cell ...the synthesis of human globin chains by a cell-free system derived from Krebs mouse ascites ...beta thalassemia messenger RNA, ... See full document
7
Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro
... made viral RNAs. The involvement of VPg in encapsida- tion has been previously proposed by Reuer et al. [15] who observed that some lethal VPg mutations still permit near normal minus and plus strand RNA synthesis ... See full document
19
LOCALIZED MUTAGENESIS FOR THE ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF ESCHERICHIA COLI AFFECTED IN PROTEIN SYNTHESIS
... The reduction in the rate of in viuo protein synthesis observed in all of these strains, together with the observed alterations in antibiotic sensitivity or ribosome assembly found[r] ... See full document
13
Ribosome Profiling Reveals HSP90 Inhibitor Effects on Stage-Specific Protein Synthesis in Leishmania donovani
... The Leishmania HSP families comprise members that are expressed constitutively during both life cycle stages, e.g., HSP70 and HSP90 (30), and others whose expression increases during the conversion into the amastigote ... See full document
18
Cell Structure and History (1).ppt
... Nucleolus Nucleus Nuclear envelope Ribosome (attached) Ribosome (free) Cell Membrane Rough endoplasmic reticulum Golgi apparatus Mitochondrion Smooth endoplasmic reticulum Centrioles.[r] ... See full document
94
Protein Synthesis in Cell-Free Systems: an Effect of Interferon
... Stimulation of amino acid incorporation by EMC RNA with untreated "74S" ribosomes from control chick embryo fibroblasts CEF, U and CEF which had been exposed to 35 0 and 140 A units of i[r] ... See full document
12
Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
... While translation initiation mediated by the cricket paralysis virus (CrPV) IRES requires only the 40S and 60S ribosomes (13, 25), both cap-dependent and CrPV IRES-mediated trans- lation require the same factors for the ... See full document
6
Glial and Neuronal Functions of the Drosophila Homolog of the Human SWI/SNF Gene ATR-X (DATR-X) and the jing Zinc-Finger Gene Specify the Lateral Positioning of Longitudinal Glia and Axons
... a protein that is an essential component of a Jing/DATR-X/JIGR1 complex or a down- stream target of these genes (S atterfield et ...and function in the embryonic Drosophila CNS (Z hang et ... See full document
19
DNA binding properties of simian virus 40 T-antigens synthesized in vivo and in vitro.
... Premixing the labeled reticulocyte cell-free products with the same amount of infected-cell extract normally used in a DNA binding experiment, representing a great excess over that synth[r] ... See full document
8
Ligation free ribosome profiling of cell type specific translation in the brain
... ligation-free ribosome profiling to measure genome-wide translation in the forebrains of adult ...RNA-Seq, ribosome foot- prints map to the transcriptome with a three-nucleotide periodicity due to ... See full document
15
Viral Proteins Formed in a Cell-Free RABBIT Reticulocyte System Programmed with RNA from a Temperature-Sensitive Mutant of Sindbis Virus
... We expected that in vitro translation of the mRNA from a temperature-sensitive mutant unable to make capsid or discrete envelope protein at the nonpermissive temperature would provide fu[r] ... See full document
8
Rhinovirus multistranded RNA: dependence of the replicative form on the presence of actinomycin D.
... Inhibition of protein synthesis in reticulocyte lysates by double-stranded and multistranded RNAs from rhinovirus-infected and noninfected HeLa cells 931 virus replication structure foun[r] ... See full document
7
Evidence that vesicular stomatitis virus produces double-stranded RNA that inhibits protein synthesis in a reticulocyte lysate.
... Effect of melting and micrococcal nuclease on the residual fractional capacity of polyA+ RNA from VSV-infected HeLa cells and polyI:polyC to inhibit protein synthesis by reticulocyte lys[r] ... See full document
10
In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System
... To analyze the in vitro synthesized products extract was prepared essentially as described by on sodium dodecyl sulfate SDS-polyacrylamide Davies and Kaesberg 5, with a few modifications[r] ... See full document
7
Coronavirus JHM: Cell-Free Synthesis of Structural Protein p60
... The virus was propagated twice in suckling mouse brain, isolated, plaque purified four times on L929 cells, and then grown in Sac- cells at low multiplic10 Downloaded from http://jvi.asm[r] ... See full document
8
Hemoglobin synthesis in β thalassemia: the properties of the free α chains
... the free a-chains are readily recovered in the HbA ...the free a-chains are able to combine normally with b-chains to form ...The free a-chains eluted as a broad peak migrating between myoglobin and ... See full document
8
Proteolytic processing of foot-and-mouth disease virus polyproteins expressed in a cell-free system from clone-derived transcripts.
... Cell-free translation of foot-and-mouth disease virus FMDV RNA in a rabbit reticulocyte system produces functional proteins, including viral protease 3C, which plays a major role in proc[r] ... See full document
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