[PDF] Top 20 Use of pulsed field gel electrophoresis for epidemiological study of Bordetella pertussis in a whooping cough outbreak

... We used pulsed-field gel electrophoresis PFGE of chromosomal DNA digested with XbaI to determine the distribution of different BordeteUa pertussis strains from clinical isolates obtained[r] ... See full document

... This study demonstrates that practical molecular biology- based epidemiological approaches can be used to identify the source and route of infection of food-borne ETEC poisoning in the ...biology-based ... See full document

... this study, we evaluated a standardized procedure for determination of the comparability of results within and be- tween ...for use as an internal reference in transforming DNA fragment migration distances ... See full document

... countries use different vaccination schedules and use at least partly different vaccines (Table ...For epidemiological characterization of B. pertussis isolates, various DNA-based techniques ... See full document

... this study, the performance (typeability, discriminatory power, reproducibility, workflow, and costs) of the SCRA system was evaluated for typing of MRSA strains isolated from patients and patients’ household ... See full document

... Pulsed-field gel electrophoresis (PFGE) has been used as an epidemiological tool for surveillance studies of Bordetella pertussis since the early ...B. pertussis ... See full document

... countries use different vaccination strategies against pertussis (11) (Table ...whole-cell pertussis vaccines have been re- placed by acellular vaccines in which only 1 to 5 protein compo- nents (in ... See full document

... published study of PFGE typing among the clinical strains of ...this study support the viewpoint indicating clonal spread as the main reason for the increasing trend of imipenem, meropenem resistance as ... See full document

... in outbreak detection and ...using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), multilocus variable-number tandem-repeat analysis (MLVA), binary typing, ... See full document

... during epidemiological studies of outbreaks in hospitals or communities during a relatively short period of time (a few months), as we had in our ...our study PFGE has showed completely eff ... See full document

... In recent years, PFGE has become a useful tool for the typing and differentiation of strains for epidemiological studies with multiple bacterial species (5, 11, 14, 20). PFGE has been used for ... See full document

... Whooping cough diagnosis: a clinical evaluation ofcomplementing culture and immunofluorescence with enzyme-linked immunosorbent assay of pertussis immunoglobulin A in nasopharyngeal secr[r] ... See full document

... A main advantage of PhP typing is that it is simple to per- form, and data processing and presentation are automated. Less than 8 h of labor, in total, including data interpretation and presentation, is required to assay ... See full document

... a certain level of discrimination power can be expected, as we demonstrate in the present study. Second, the analysis can be performed without the need for special equipment or tech- niques, requiring only ... See full document

... Optical mapping. Optical maps for each isolate were prepared from cells of single 1-mm colony equivalents following growth on Regan-Lowe agar without cephalexin using the Argus system (OpGen, Gaithersburg, MD) according ... See full document

... farm environments and/or cattle herds associated with human cases have allowed investigators to establish relationships be- tween animal and human isolates (7, 16, 34, 68; W. B. Trevena, R. S. Hooper, C. Wray, G. A. ... See full document

... Our study showed PFGE to be superior to serotyping, which is the currently recommended method for providing laboratory links to epidemiologic ...one study (23) and in 10% of isolates in another ... See full document

... We report here on an outbreak of B. pickettii bacteremias at a 650-bed teaching hospital in Liege, Belgium. From June to August 1994, six immunocompromised patients developed B. pickettii bacteremia and/or ... See full document

... from outbreak strains using PFGE with both restriction ...from outbreak 1, which occurred in ...from outbreak 4 in Inuvik (isolates FE0005EJT and ...the outbreak strains, they originated from ... See full document

... The results indicate that determination of specific IgA to PT and FHA in NPH aspirates represents a sensitive and rapid diagnostic method for the detection of pertussis.... GRANSTROM ET [r] ... See full document