[PDF] Top 20 Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.
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Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.
... Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oriS replication origin.. Genetic analysis of temperature-sensitive mutants w[r] ... See full document
10
Helicase Motif Ia Is Involved in Single-Strand DNA-Binding and Helicase Activities of the Herpes Simplex Virus Type 1 Origin-Binding Protein, UL9
... during protein purification. Fractions con- taining UL9 were identified with the Bradford protein assay and analyzed fur- ...of UL9 protein and proteinase K at a concentration of ... See full document
12
Direct Interaction between the N- and C-Terminal Portions of the Herpes Simplex Virus Type 1 Origin Binding Protein UL9 Implies the Formation of a Head-to-Tail Dimer
... DNA binding domain forms a monomer in solution (12); however, the full-length protein exists as a dimer under these conditions (6, ...of UL9 contain a putative leucine zipper similar to those ... See full document
9
Herpes simplex virus type 1 origin-specific binding protein: oriS-binding properties and effects of cellular proteins.
... Incubation of infected cell extracts with the lower-affinity site II DNA as a probe resulted in the appearance of two protein-DNA complexes with mobilities identical to those of the A an[r] ... See full document
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Purification and characterization of herpes simplex virus type 1 alkaline exonuclease expressed in Escherichia coli.
... 86-kDa protein purified from bacterial cells was indeed the AE coded for by HSV-1, an affinity-purified rabbit polyclonal antiserum that was raised against electroeluted rAE protein (antiserum BWp12) ... See full document
6
Purification of the herpes simplex virus type 1 65-kilodalton DNA-binding protein: properties of the protein and evidence of its association with the virus-encoded DNA polymerase.
... 8 Purification of the Herpes Simplex Virus Type 1 65-Kilodalton DNA-Binding Protein: Properties of the Protein and Evidence of Its Association with the Virus-Encoded DNA Polymerase MICHA[r] ... See full document
10
Expression, purification of herpes simplex virus type 1 US11 Protein, and production of US11 polyclonal antibody
... US11 protein is a 21 kDa, highly basic phosphopro- tein [4], and is also an RNA-binding protein, post-tran- scriptional regulator of gene expression ...interacting protein kinase 2 (HIPK2) ... See full document
6
The conserved helicase motifs of the herpes simplex virus type 1 origin-binding protein UL9 are important for function.
... Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replication.. Nucleic Acids Res.[r] ... See full document
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Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein
... of origin- independent ...wild-type virus after coinfection with ts ...wild type at an MOI of ...wt virus at an MOI of ...wt virus in the double infections and of an MOI of ... See full document
6
Origin Binding Protein-Containing Protein-DNA Complex Formation at Herpes Simplex Virus Type 1 oriS: Role in oriS-Dependent DNA Replication
... on origin-dependent HSV DNA replication, the mutOBP and mutR(C-G) mutations were in- troduced into the wild-type oriS-containing plasmid, pOS822, and the effects of these mutations were evaluated in in ... See full document
9
Purification and Characterization of a Cellular Protein That Binds to the Downstream Activation Sequence of the Strict Late UL38 Promoter of Herpes Simplex Virus Type 1
... 2. Purification and identification of DBF as the DNA binding component of ...for purification of DBF from uninfected HeLa nuclear extracts ...shift binding buffer (10 mM HEPES [pH ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... Cluster 1 is located in the apical region of the protein lo- cated on the same face as the view shown in ...cluster 1, traverses laterally across the entire molecule and abuts disordered loop ... See full document
9
Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
8
Properties of the novel herpes simplex virus type 1 origin binding protein, OBPC.
... (pCMVOBPC 1 ) inhibited pOS822 replication by 65%, whereas the vector plasmid alone (pCMV) and the plasmid containing OBPC sequences in the negative orientation (pCMVOBPC 2 ) had no effect on the level of pOS822 ... See full document
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Characterization of major recognition sequences for a herpes simplex virus type 1 origin-binding protein.
... 4096-4103 0022-538X/88/114096-08$02.00/0 Copyright C 1988, American Society for Microbiology Characterization of Major Recognition Sequences for a Herpes Simplex Virus Type 1 Origin-Bind[r] ... See full document
8
Origin of Expression of the Herpes Simplex Virus Type 1 Protein US1.5
... been reported recently (4), although reagents capable of de- tecting expression of U S 1.5 using these procedures were not available. In order to determine if U S 1.5 can be expressed in transient assays in the presence ... See full document
12
Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... cell type, it should be noted that we were unable to detect phos- phorylation of OBP in Vero cells transfected with an OBP expression plasmid (pCMVUL9; data not shown), indicating that cellular kinases active ... See full document
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Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains.
... a protein (reviewed in reference 18). Isolation and characterization of transdomi- nant mutants of several HSV-1 proteins, including the trans- activators ICP0, ICP4, and VP16, have facilitated the ... See full document
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DNA Binding Activity of the Herpes Simplex Virus Type 1 Origin Binding Protein, UL9, Can Be Modulated by Sequences in the N Terminus: Correlation between Transdominance and DNA Binding
... of UL9 were ...terminus, UL9-394-851 and UL9-304-851, respectively, were able to partially relieve the inhibitory effects exhibited by C-terminal UL9 and by wild-type UL9 itself, ... See full document
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