[PDF] Top 20 Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real Time PCR
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Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real Time PCR
... Human immunodeficiency virus type 2 (HIV-2) was first isolated in 1986 from peripheral blood mononuclear cells (PBMC) from patients in the Cape Verde Islands and Guinea- Bissau ... See full document
5
Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... subtype B, the most frequent subtype found in the European population, representing 80 to 85% of the strains isolated in ...amplify subtypes A, C, and ...tested, using one strain for each type ... See full document
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Reduced proviral loads during primo infection of sheep by Bovine Leukemia virus attenuated mutants
... the proviral loads, viral DNA lev- els within the circulating blood of all sheep were deter- mined by semiquantitative ...by PCR. The number of PCR cycles was adapted in order to compare the relative ... See full document
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Development and Validation of a Multiplex Real Time PCR Assay for Simultaneous Genotyping and Human T Lymphotropic Virus Type 1, 2, and 3 Proviral Load Determination
... The human T-lymphotropic virus (HTLV) proviral load remains the best surrogate marker for disease ...progression. Real-time PCR techniques have been developed for ... See full document
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An antiretroviral drug naïve human immunodeficiency virus 1 infected woman with a persistent non reactive proviral deoxyribonucleic acid polymerase chain reaction: a case report
... two time points when tested for HIV pro- viral DNA up to 7 months after an unprotected hetero- sexual ...HIV proviral DNA ...HIV proviral DNA PCR was negative in this woman who had indicated ... See full document
6
Detection of human immunodeficiency virus type 1 proviral DNA by PCR using an electrochemiluminescence tagged probe
... by using an auto- mated instrument, the Origen-I ...at 2 20 8 C, eliminating the need to prepare small amounts of freshly labeled probe every 2 ...of PCR products is significantly simpler and ... See full document
6
MultiCode RTx Real Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples
... of PCR. (B) Reaction ...Step 2 is allele-specific extension that relies on differ- entiation between K65R and K65K codons based on an only 1-base ...of real-time PCR datum set ... See full document
5
Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... HIV-1 proviral DNA in PBMC, plasma HIV-1 concentrations can change dynamically in response to treat- ment (7), in contrast to cellular proviral DNA ... See full document
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Development of Calibrated Viral Load Standards for Group M Subtypes of Human Immunodeficiency Virus Type 1 and Performance of an Improved AMPLICOR HIV 1 MONITOR Test with Isolates of Diverse Subtypes
... plasma human immunodeficiency virus type 1 (HIV-1) RNA levels is critical for the effective management of HIV-1 ...transcription- PCR-based test for quantification of HIV-1 RNA ... See full document
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Genotyping of B and Non B Subtypes of Human Immunodeficiency Virus Type 1
... viral load of ⬎ 1,000 copies/ml, as determined by the branched chain DNA assay ...by using the QIAGEN viral RNA extraction kit (QIAGEN, Hilden, ...RNA using the primer sets supplied by the ...by ... See full document
5
Real Time PCR Assay for Clinical Management of Human Immunodeficiency Virus Infected Patients with Visceral Leishmaniasis
... a real-time PCR for Leishmania DNA in the diagnosis and follow-up of patients with human immunodeficiency virus type 1 (HIV-1) and Leishmania coinfection, Leishmania DNA ... See full document
6
Rapid, Point of Care Extraction of Human Immunodeficiency Virus Type 1 Proviral DNA from Whole Blood for Detection by Real Time PCR
... viral load for this time point was 77,900 copies/ml, and specimens from this patient were positive at both an earlier time point and a later time point, indicating that the assay could detect ... See full document
6
Performance of a Novel Human Immunodeficiency Virus (HIV) Type 1 Total Nucleic Acid Based Real Time PCR Assay Using Whole Blood and Dried Blood Spots for Diagnosis of HIV in Infants
... DBS using the HIV-1 secondary standard showed an excellent LOD of 1,090 copies/ml with an equiva- lent input of 60 l of whole blood, which would improve with higher input ...with proviral HIV-1 DNA, thus ... See full document
5
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... transcriptase PCR assay (3), has been widely used in research as well as in clinical ...practice. Quantification of HIV-1 RNA with this assay requires two sample preparation procedures: in the first, ... See full document
5
Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real Time PCR
... The real-time PCR allows a simplified protocol with fewer steps than classical ...and B are prevalent, the epi- demic being more restricted than that for HIV-1 group ...group B samples ... See full document
5
Human immunodeficiency virus type 1 (HIV 1) proviral DNA load in purified CD4+ cells by LightCycler® Real time PCR
... viral load, allowing follow up of the immediate effects of treatment, and CD4+ T cell count, allowing initiation or reinitiation of the therapy as immune function decreases ...HIV-1 proviral DNA load ... See full document
9
Development and Assessment of a Multiplex Real Time PCR Assay for Quantification of Human Immunodeficiency Virus Type 1 DNA
... several PCR-based methodologies had been developed for the quantification of cell-associated HIV-1 DNA, based on end-point and real-time PCR methodology ...of real-time ... See full document
6
Diversity of Human Immunodeficiency Virus Type 1 Subtypes in Western Kenya
... pure subtypes of HIV-1 group M are currently known (A-D, F-H, J and ...these subtypes are further subdivided into sub-subtypes e.g. subtypes F (F1 and F2); A (A1, A2 and ...of virus ... See full document
9
Validation and implementation of hepatitis b viral load detection with quantitative real time PCR
... nearly 2 billion people are infected by HBV worldwide (Yim and Lok, HBV DNA viral load detection is required for the proper management of the chronic Hepatitis B patients for cient, reliable and ... See full document
5
Real Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G
... DNA quantification, including hybrid capture and branched DNA (bDNA) signal amplification (19–21, 35), assays based on real- time PCR technology (12) offer the greatest sensitivity and broad- ... See full document
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