[PDF] Top 20 Quantification of Substance P mRNA in Human Immune Cells by Real-Time Reverse Transcriptase PCR Assay
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Quantification of Substance P mRNA in Human Immune Cells by Real-Time Reverse Transcriptase PCR Assay
... developed real-time reverse transcriptase (RT) PCR (RT-PCR) assay for quanti- fication of substance P (SP) mRNA expression (the SP ... See full document
6
Quantification of CCR5 mRNA in Human Lymphocytes and Macrophages by Real-Time Reverse Transcriptase PCR Assay
... The PCR buffer contained 5-carboxy-X-rhodamine (500 nM) as the reference dye for normalization of the ...the real-time PCR ...CCR5 mRNA in human immune cells, known ... See full document
6
Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... infected cells, generating a 119-bp fragment and a 188-bp fragment, ...and reverse (DENV R1 and CHIKV R1) primers are listed in Table ...the PCR product was used to clone amplicons into TOPO TA ... See full document
8
Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... the assay more laborious and ...a real-time QT-NASBA format can be constructed (17). This real-time QT-NASBA assay is very promising, since it com- bines the isothermal ... See full document
6
Development and Evaluation of a Real-Time Reverse Transcription-PCR Assay for Quantification of Gamma Interferon mRNA To Diagnose Tuberculosis in Multiple Animal Species
... ␥ mRNA as an indicator of TB infection, the qRT-PCR assay was validated for use with a single target species experimentally infected with ...IFN-␥ mRNA, IFN-␥ protein, and lymphocyte ... See full document
9
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... quantitative reverse transcriptase PCR assay (3), has been widely used in research as well as in clinical ...practice. Quantification of HIV-1 RNA with this assay requires two ... See full document
5
Real-Time Reverse Transcription-PCR Quantitation of Substance P Receptor (NK-1R) mRNA
... Substance P (SP), the most extensively studied and potent member of the tachykinin family, is a modulator of neuroim- munoregulation, in particular, the immune functions of mono- nuclear phagocytes ... See full document
5
MultiCode RTx Real Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples
... Human immunodeficiency virus type 1 (HIV-1) exhibits tremendous genetic variation, allowing virus to escape host immune response and develop drug resistance to antiretro- viral therapeutics (1). To manage ... See full document
5
Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real Time PCR Assay
... of immune-suppressive chemotherapy has enabled the number of transplants performed to increase, and these patients all require ...using real-time Light Cycler ... See full document
6
Validation of a pan orthopox real time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
... quantitative real time PCR assay validated for the determination of monkeypox viral GC levels in cynomolgus macaque EDTA whole blood (Table ...The assay was designed to support the ... See full document
9
Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific ... See full document
9
Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... blood cells, the ability to detect only nonequivalent molar concentrations up to 100-fold may be problematic (6, ...blood cells, which are the site of infection for ...circulating cells in blood ... See full document
10
Development of a Real Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
... RRT- PCR did not have any sequence variation in the probe binding region, and the single base change present in the M⫺124 primer is present in many other isolates that were detected with these primers, including ... See full document
5
Lipodystrophy among HIV-infected patients: a cross-sectional study on impact on quality of life and mental health disorders
... (95% CI:1.33–29.40); M2: OR = 5.22 (95% CI: 1.07– 25.37)] were all associated with a poor quality of life in the univariate model (Table 4). They remained statisti- cally significant in both multivariate analyses, apart ... See full document
10
Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... TaqMan assay to detect coinfection of multiple ...this assay was com- pared to that of the single-dye, four-tube TaqMan ...NS5 assay, ...C-prM assay agreed with the virus isolation record, the ... See full document
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A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... The PCR master mix was distributed in Snap-2 and Snap-3 tubes on the DNA-3 extraction ...lyophilized PCR reagent mix and the primers and probe for the sample processing control ...Max PCR cartridge ... See full document
8
Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... Kreher P, Lattwein E, Eschbach-Bludau M, Nitsche A, Bleicker T, Landt O, Schweiger B, Drexler JF, Osterhaus AD, Haagmans BL, Dittmer U, Bonin F, Wolff T, Drosten C (2012) Assays for laboratory confirmation of ... See full document
5
Rapid Detection of West Nile Virus from Human Clinical Specimens, Field Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase PCR Assay
... immunofluorescence assay with WN virus-specific monoclonal antibodies can also yield unam- biguous ...tissues. Reverse transcriptase (RT)-PCR has been used to develop highly sensitive and ... See full document
6
A novel real time PCR assay for specific detection and quantification of Mycobacterium avium subsp paratuberculosis in milk with the inherent possibility of differentiation between viable and dead cells
... Austria). Cells were declumped by passing the suspension three times through a disposable insulin syringe (Omnican®40; ...min. Cells remaining in the supernatant were pelleted at 8,000 × g for 5 min and ... See full document
8
New Real Time Reverse Transcriptase Initiated PCR Assay with Single Copy Sensitivity for Human Immunodeficiency Virus Type 1 RNA in Plasma
... the assay is tolerant of small numbers of mismatches between the primers and the ...the assay may not work as well with non-subtype B viruses and that primer and probe sequences would need to be redesigned ... See full document
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