[PDF] Top 20 Rapid Identification of Candida Species with Species Specific DNA Probes
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Rapid Identification of Candida Species with Species Specific DNA Probes
... digoxigenin-labeled probes. Eighteen Candida species probes (Table 1) were designed and tested in the PCR- EIA against the DNAs from the fungi listed in Table ...homologous DNA was ... See full document
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Rapid Identification of Candida Species by Confocal Raman Microspectroscopy
... Candida species are important nosocomial pathogens associated with high mortality ...rates. Rapid detection and identification of Candida species can guide a clinician at an ... See full document
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Rapid identification of Candida species in blood cultures by a clinically useful PCR method
... by Candida albicans but has also resulted in the emergence of candi- demias caused by innately fluconazole-resistant, ...candidiasis, rapid identification of newly emerging Candida ... See full document
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Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L borgpetersenii, and L kirschneri) with species specific DNA probes produced by arbitrarily primed PCR
... between species of ...revealed species-specific DNA fragments. These species-specific sequences were reamplified in order to produce digoxigenin-11-dUTP-labeled genomic ... See full document
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Rapid Identification of Candida Species and Other Clinically Important Yeast Species by Flow Cytometry
... yeast species in a single-well test within one working day when starting from ...designing probes for the direct hybridization method, there were few consistent rules that appeared to apply to the ... See full document
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Rapid identification of Candida species by DNA fingerprinting with PCR
... Genomic DNA was extracted by three different ...of DNA, the method of Gruber (12) was used as described previously ...of DNA are needed for PCR fingerprinting, two minipreparation methods were used ... See full document
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Rapid Identification of Candida dubliniensis Using a Species Specific Molecular Beacon
... sites. DNA sequence analysis of the internal transcribed spacer 2 (ITS2) region of rRNA genes from reference Candida strains was used to develop molecular beacon probes for rapid, ... See full document
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Rapid Identification of up to ThreeCandida Species in a Single Reaction Tube by a 5′ Exonuclease Assay Using Fluorescent DNA Probes
... fungal DNA from whole blood (3, 7, 12), most require initial lysis of erythrocytes and leukocytes with salts, detergents, and/or proteinase K and multiple purification steps in order to obtain adequate DNA ... See full document
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Rapid identification and fingerprinting of Candida krusei by PCR based amplification of the species specific repetitive polymorphic sequence CKRS 1
... the species-specific CKRS-1 repetitive polymorphic se- quence of the intergenic region of rRNA genes in ...is species spe- cific and ...and identification of Candida species have ... See full document
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Rapid Identification of Dimorphic and Yeast Like Fungal Pathogens Using Specific DNA Probes
... oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in ...ribosomal DNA, were used to amplify DNA from Histoplasma capsulatum, Blastomyces ... See full document
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Rapid identification of non candida albicans candida (ncac) species isolated from female patients clinically diagnosed with vulvovaginitis by using multiplex pcr
... these Candida species on the same medium that characterized by (Calderone, 2002), which were blue-green colonies for ...related species C. albicans and Candida dubliniensis, oral rinses of ... See full document
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Species Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology
... fungal species in blood cultures and clini- cal ...fungal species in Candida-positive blood cultures obtained from 10 ...of DNA from the respective blood cultures and seminested PCR ... See full document
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Reverse Cross Blot Hybridization Assay for Rapid Detection of PCR Amplified DNA from Candida Species, Cryptococcus neoformans, and Saccharomyces cerevisiae in Clinical Samples
... designed probes based on the ERG11 gene region chosen for PCR amplifi- ...these probes, many length combinations for each probe were evaluated to obtain probes with comparable annealing ...The ... See full document
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Rapid Detection and Identification ofCandida, Aspergillus, and FusariumSpecies in Ocular Samples Using Nested PCR
... the rapid detection of fungal DNA in ocular samples, derived from three species, Candida albicans, Aspergillus fumigatus, and Fusarium solani, has been ...three species were ... See full document
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Species Specific and Ubiquitous DNA Based Assays for Rapid Identification of Staphylococcus aureus
... aureus-specific DNA-based diagnostic tests. A genomic DNA probe which was specific and ubiquitous for the identification of ...442-bp DNA probe did not show any significant ... See full document
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Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates
... Although Candida albicans remains the most common cause of fungemia and disseminated candidiasis, other Candida spe- cies are not uncommon, accounting for about 25 to 60% of systemic candidal infections in ... See full document
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Development of two species specific fingerprinting probes for broad computer assisted epidemiological studies of Candida tropicalis
... of DNA from ...genomic DNA or a ribosomal DNA probe of ...for DNA hybridization with genomic DNA were performed under non- saturating conditions, clones containing repeat sequences gen- ... See full document
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Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
... The C. albicans cell wall is a rigid matrix that mainly consists of polysaccharides (about 80- 90%) represented by β-1,3 and β-1,6 glucans, chitin and mannan. Proteins (6-25%) and a small amount of lipid (1 to 7%) are ... See full document
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Seminested PCR for Diagnosis of Candidemia: Comparison with Culture, Antigen Detection, and Biochemical Methods for Species Identification
... for Candida detection and species identification directly in clinical speci- ...of Candida DNA in the serum, thereby reducing the possibility of false ...two Candida ... See full document
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Species specific and ubiquitous DNA based assays for rapid identification of Staphylococcus epidermidis
... genomic DNA frag- ...carrying species-specific DNA inserts by hybridization to DNAs from an array of bacterial species (Table ...a species-specific probe. For each clone, ... See full document
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