[PDF] Top 20 Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA
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Use of a reamplification protocol improves sensitivity of detection of Mycobacterium tuberculosis in clinical samples by amplification of DNA
... We have compared the sensitivity and specificity of quantitative mycobacterial culture against results obtained by using the polymerase chain reaction for the detection of DNA from organ[r] ... See full document
6
Novel Real Time Simultaneous Amplification and Testing Method To Accurately and Rapidly Detect Mycobacterium tuberculosis Complex
... the detection target is ...than DNA amplifica- tion products. Since PCR detection commonly suffers from severe contamination problems, the SAT-TB assay should reduce the risk of laboratory ... See full document
5
Detection of Mycobacterium tuberculosis in respiratory specimens by strand displacement amplification of DNA
... The cutoff used for rejecting suppressed SDA reactions was empirically determined to eliminate the 5% of culture-negative specimens with the lowest SDA SIC RLU results. Using the SIC cutoff on the 75 MTB culture-positive ... See full document
6
Direct Detection of Mycobacterial Species in Pulmonary Specimens by Two Rapid Amplification Tests, the Gen Probe Amplified Mycobacterium tuberculosis Direct Test and the GenoType Mycobacteria Direct Test
... monary samples from 65 patients suspected to suffer from TB or another ...M. tuberculosis test (Roche Diag- nostic Systems) as the reference ...a sensitivity level of 80.5% and a specificity level of ... See full document
5
Rapid Detection of Mycobacterium tuberculosis in Respiratory Samples by Transcription Reverse Transcription Concerted Reaction with an Automated System
... for detection of MTC in clinical sputum samples by cul- ture, TRC method, and COBAS AMPLICOR ...PCR. Clinical samples were ob- tained from patients diagnosed with or suspected of having ... See full document
5
Comparison of an Internally Controlled, Large Volume LightCycler Assay for Detection of Mycobacterium tuberculosis in Clinical Samples with the COBAS AMPLICOR Assay
... Microbial strains. DNAs from various bacteria and fungi used for specificity testing were obtained from the culture collection of the Institut fu ¨r Medizinische Mikrobiologie und Hygiene at the University of Regensburg, ... See full document
6
Relevance of Commercial Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Clinical Samples
... that use and/or implementation of CDATs should take into account the level of laboratory service and should also be based on cost-effective ...early detection and treatment of patients and appropriate ... See full document
11
Detection and Identification of Mycobacterium Species Isolates by DNA Microarray
... of Mycobacterium species isolates is necessary for the effective management of tuber- ...of DNA gyrase B subunit (gyrB) genes has been identified as a suitable means for the identification of bacterial ... See full document
11
Clinical Evaluation of TRCRapid M TB for Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Specimens
... of tuberculosis is crucial to providing optimal treatment and reducing the spread of ...nonrespiratory clinical specimens using a new automated Mycobacterium tuberculosis complex (MTBC) rRNA ... See full document
6
PCR Restriction Fragment Length Polymorphism for Rapid, Low Cost Identification of Isoniazid Resistant Mycobacterium tuberculosis
... 80% sensitivity in the routine identifica- tion of isoniazid-resistant ...M. tuberculosis DNA, shown by katG sequencing, in the original culture ex- tract of isolate RE180 suggests that a laboratory ... See full document
5
Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species
... Kb DNA fragment from the mycobacterial 16S rRNA genes, which are highly stable rather than insertion elements which are likely to undergo deletions Radhakrishnan, I ...96.3% sensitivity and ...of ... See full document
9
Investigation of Electrochemical Behaviour of Chromium(VI)-Dithiocarbamate Complexes: Detection of Chromium(VI) in Real Samples
... Chennai, India. All the samples were prior concentrated to 100 ml for easy detection of analyte. The obtained results are tabulated in Tables 3-4. 10 g of Triticum aestivum (Wheat) and 5 g of Spinacia ... See full document
8
Multiplex Real Time PCR Melting Curve Assay To Detect Drug Resistant Mutations of Mycobacterium tuberculosis
... rapid detection of drug- resistant ...M. tuberculosis can be detected in a one-tube reaction by using the beacons (7, ...short DNA regions, mul- tiple probes (more than four) labeled with different ... See full document
7
Amplification of a species specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis
... Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis.. de la Maza.[r] ... See full document
8
Genetic Determinants of Drug Resistant Tuberculosis among HIV Infected Patients in Nigeria
... In Nigeria, HIV/TB coinfection rates are as high as 30% in anti- retroviral therapy (ART) clinic settings, and the national preva- lence of MDR-TB is unknown. Our results show high rates of transmitted drug-resistant TB ... See full document
5
Pneumocystis carinii f sp hominisDNA in Immunocompetent Health Care Workers in Contact with Patients with P carinii Pneumonia
... The samples used in this study were collected in the early ...of tuberculosis, and currently HIV-infected patients with any respiratory symp- toms, including those with suspected or confirmed ... See full document
6
Could high-concentration rifampicin kill rifampicin-resistant M. tuberculosis? Rifampicin MIC test in rifampicin-resistant isolates from patients with osteoarticular tuberculosis
... The mechanisms underlying drug resistance are often multifaceted and may include not only chromosomal mutations but also induction or the presence of efflux pumps, and even antagonism of the component drugs in ... See full document
5
Performance Assessment of Two Commercial Amplification Assays for Direct Detection of Mycobacterium tuberculosis Complex from Respiratory and Extrapulmonary Specimens
... and clinical diagnosis as the “gold ...121 Mycobacterium tuberculosis complex (MTB) (98 smear-positive), 46 nontuberculous mycobacteria (38 smear-positive), and 338 culture-negative ...percent ... See full document
5
Prevalence of Tuberculosis in Patients of Diabetes Mellitus: a Review
... and tuberculosis are having age over 40 years and are meaningfully old aged as compared to patients having no diabetes [31] The prevalence of type 2 diabetes is greater in old aged patients ...and ... See full document
7
A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
... Our samples were four sputum samples 1 ml per portion, one liver biopsy sample 10 mg per portion, and one feces sample 600 ,ul per portion from patients suffering from diseases other tha[r] ... See full document
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