[PDF] Top 20 Retention of plasmid DNA in mammalian cells is enhanced by binding of the Epstein-Barr virus replication protein EBNA1.

... Cell lines expressing either EBNA1 solid bars; a deletion mutant expressing the DNA binding domain, nuclear localization sequence, and acidic carboxy terminus EBDB, hatched bar; or neith[r] ... See full document

... The EBNA1 325–376 region has been shown to mediate an interaction with one cellular protein that is associated with mitotic chromosomes, termed EBP2 (EBNA1 binding protein 2) ...in ... See full document

... ZKO-293 cells (a gift from H. J. Delecluse) are 293 cells transformed with hygromycin-resistant EBV bacmid with a deletion of the BZLF1 gene and were grown in RPMI medium with 10% fetal bovine serum (FBS) ... See full document

... in binding to methylated CpGs that are embedded in two ZEBRA response elements (ZREs) of Rp (38, ...BZKO cells, a 293 cell line harboring an EBV bacmid that lacks a functional gene for ZEBRA, were ... See full document

... 7.2-kb plasmid that contains oriP and that confers on human cells resistance to hygromycin B ...individual EBNA1 binding sites and the 5- and 10-bp insertion mutations (16) were kindly ... See full document

... wild-type EBNA1 or dn1160, a dominant-neg- ative derivative of EBNA1 that is little more than the DNA-bind- ing domain (originally called ⌵⌬ 450 – 641 ...preventing plasmid loss, N-terminal ... See full document

... (Qp), EBNA1 binds to two 18 bp sequences immediately downstream of the transcriptional start site, and functions as an inhi- bitor of transcription initiation and mRNA accumulation ...[9]. EBNA1 binds ... See full document

... Epstein-Barr virus (EBV) is present in tumor cells of more than 90% of cases of Burkitt’s lymphoma (BL) in areas of endemicity in equatorial Africa and New Guinea, as well as in tumor ... See full document

... coli onC, the SV40 origin, and yeast origins, since the dyad symmetry region contains an easily unwound sequence as well as binding sites for an essential replication protein EBNA1.. How[r] ... See full document

... 293T cells transfected with either BBLF4-GFP or a GFP control vector were incubated with glutathione beads containing equal amounts of the different GST-Zta ...detect protein binding to the GST-Zta ... See full document

... EBNA1 protein bound to a plasmid containing FR, the herpes simplex virus type 1 thymidine kinase promoter, and the firefly luciferase gene was electroporated into cells.. Downloaded from[r] ... See full document

... The EBNA1 protein of Epstein-Barr virus (EBV) governs the replication and segregation of the viral episomes in latently infected cells and transactivates the expression of ... See full document

... Some authors 1, 27 recently reported that induced or uninduced Raji cells do not contain any DNA polymerase activity and concluded that the lack of this enzyme is the reason for the inca[r] ... See full document

... BALF5 protein translation is primarily dependent on the length of the 3= ...of DNA replication likely contributes to the M81 lytic ...BALF5 protein-coding sequences from a variety of EBV ... See full document

... of protein com- plexes near the splice junction (for review, see reference ...By binding to viral mRNAs and to cellular export proteins simultaneously, SM family members could provide access for unspliced ... See full document

... However, for the following reasons we presume they represent proteolytic degradation products generated in vivo: i immunoprecipitates of COS cells electroporated with pSV2CAT a plasmid s[r] ... See full document

... Adsorption of FITC-EBV and unlabeled EBV with SV-treated cells EBNA induction" in BJA-B cells by residual EBV' % EBNA positive Residual FITC-EBVa binding EBV adsorption with: to Raji cel[r] ... See full document

... Rta binding site predicted by a position- specific weight matrix (27) was only 36 nucleotides (nt), which is highly significant (P ⫽ ...displays protein-coding genes in the B95-8 genome, drawn schematically ... See full document

... Thus, the findings that there is no inverted repeat within 10 min of lambda exonuclease digestion, the size 10 x 106 daltons of the terminus of the long of most EBV DNA molecules was bet[r] ... See full document

... Although region I is required for the transient replication of plasmids bearing region II in EBV-infected B cells, a plasmid lacking region I but containing region II, was observed to re[r] ... See full document