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[PDF] Top 20 PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis

Has 10000 "PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis" found on our website. Below are the top 20 most common "PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis".

PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis

PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis

... as PCR, real-time PCR, and microarray analyses, are used to detect pathogens, and these methods have shown enhanced sensitivities and specificities for microbial pathogens ...of sepsis, ... See full document

7

Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens

Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens

... probe hybridization technology has been successfully used for identification of fungal isolates (1, 2, 8, 23, 24), species identification from clinical specimens has remained a chal- ...Real-time ... See full document

9

Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay

Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay

... line blot hybridization assays (RLB) have been used for the rapid diagnosis and genotyping of many ...ITS1 PCR primers and the ...ITS1 PCR and can detect ...ITS1 PCR by using 67 samples ... See full document

8

Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care

Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care

... Bioplex-based assay, 5 ␮ l of nasal swab sample was directly mixed with 20 ␮ l of PCR reagents, and the amplification, bead hybridization, washing, labeling, and flow cytometer analysis steps were ... See full document

8

Detection and Identification of EightTrichinella Genotypes  by Reverse Line Blot Hybridization

Detection and Identification of EightTrichinella Genotypes by Reverse Line Blot Hybridization

... other PCR-based methods for detecting and identifying the different Trichinella genotypes have also been ...RLB assay, labeled PCR products using only one primer set are necessary, and this enables ... See full document

5

Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay

Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay

... other pathogens. Stellrecht et al. (40), using urine and swabs for PCR, recorded sensitivities similar to those of culture for Ureaplasma ...for PCR as soon as possible after collection and, if they ... See full document

7

Reverse Cross Blot Hybridization Assay for Rapid Detection of  PCR Amplified DNA from Candida Species, Cryptococcus neoformans, and Saccharomyces cerevisiae in Clinical Samples

Reverse Cross Blot Hybridization Assay for Rapid Detection of PCR Amplified DNA from Candida Species, Cryptococcus neoformans, and Saccharomyces cerevisiae in Clinical Samples

... A PCR-based assay was developed to detect and identify medically important yeasts in clinical ...The PCR product was analyzed in a reverse cross blot hybridization assay ... See full document

6

Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay

Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay

... The reverse line blot hybridization (RLBH) assay (11, 24) is a probe-based method where multiple oligonucleotide probes carrying the mutant sequence and wild-type sequence are im- mobilized on ... See full document

6

Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization

Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization

... line blot hybridization assay. The RLB hybridization assay was mod- ified (31), based on a previously described method ...the PCR prod- ucts. If present, the relevant PCR ... See full document

5

Comparison of a Multiplex Reverse Transcription PCR Enzyme Hybridization Assay with Conventional Viral Culture and Immunofluorescence Techniques for the Detection of Seven Viral Respiratory Pathogens

Comparison of a Multiplex Reverse Transcription PCR Enzyme Hybridization Assay with Conventional Viral Culture and Immunofluorescence Techniques for the Detection of Seven Viral Respiratory Pathogens

... the identification of these viruses as causes of respiratory disease in these patients is the first step in determining how frequently they may cause serious prob- lems and, hence, how hard we should push with ... See full document

5

Development of a PCR Based Line Probe Assay for Identification of Fungal Pathogens

Development of a PCR Based Line Probe Assay for Identification of Fungal Pathogens

... a reverse-hybridization line probe assay (LiPA) which when combined with PCR amplification detects and identifies clinically significant fungal pathogens including Candida, Aspergillus, ... See full document

8

Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay

Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay

... Entamoeba reverse line blot assay could be used to detect and identify Entamoeba in samples from ...of hybridization of the PCR products obtained from samples from rural villages in ... See full document

5

Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay

Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay

... The method was further evaluated using 173 previously stud- ied (4, 7) clinical isolates from China, Australia, and Canada, which included one to four isolates of all pneumococcal sero- types except for 10C, 11F, 23B, ... See full document

5

Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay

Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay

... fluorescence PCR (24), into cultures of primary monkey kidney cells and a variety of contin- uous cell lines, including A549 (human lung carcinoma), MRC-5 (human em- bryonic lung fibroblast), and RD (human ... See full document

7

Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis

Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis

... for screening and diagnosis of ...of PCR varies, depend- ing on the target and, in particular, whether there are one or more ...five PCR assays—including two targeting the cryptic plasmid (of which ... See full document

6

Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

... hybridization assay (table 2). In 162 positive in multiplex PCR reverse cross blot hybridization assay, 149 samples gave positive results with the probes pMyc5a, pt3 and ... See full document

6

Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis

Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis

... Signs and symptoms of neonatal bacterial meningitis may be subtle, nonspecific, vague, and atypical. A high index of suspicion is therefore needed to initiate investi- gations. Further, the identification of ... See full document

8

Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology?

Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology?

... three PCR assays: a 16S PCR-based reverse line blot (RLB) assay, a single-step PCR, and a nested ...and hybridization. The PCR results by gel electrophoresis in the ... See full document

5

“Tekenscanner”: a novel smartphone application for companion animal owners and veterinarians to engage in tick and tick borne pathogen surveillance in the Netherlands

“Tekenscanner”: a novel smartphone application for companion animal owners and veterinarians to engage in tick and tick borne pathogen surveillance in the Netherlands

... After downloading the Tekenscanner app, users were asked to create an account and enter the age, sex and breed of their pet into their account. The geographical coordinates of the location where the tick was removed from ... See full document

9

Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis

Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis

... The identification of point mutations as responsible for ...probe assay (INNO-LiPA Rif TB assay; Innogenetics, Ghent, Belgium) for the detection of RIF resistance and the GenoType MTBDR assays, ... See full document

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