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[PDF] Top 20 Role of gene 2 in bacteriophage T7 DNA synthesis.

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Role of gene 2 in bacteriophage T7 DNA synthesis.

Role of gene 2 in bacteriophage T7 DNA synthesis.

... It was observed that after infection with T7 wild type a major portion of the newly synthesized DNA sedimented in both alkaline and neutral sucrose gradients 1.3 to 1.6 times as fast as [r] ... See full document

7

Synthesis of Bacteriophage and Host DNA in Toluene-Treated Cells Prepared from T4-Infected Escherichia coli: Role of Bacteriophage Gene D2a

Synthesis of Bacteriophage and Host DNA in Toluene-Treated Cells Prepared from T4-Infected Escherichia coli: Role of Bacteriophage Gene D2a

... Extension of these systems to the examination of DNA synthesis in in vitro systems prepared from Escherichia coli cells that have been infected with bacteriophage T4 might be useful in t[r] ... See full document

6

A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing

A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing

... with T7 DNA polymerase, gene ...of DNA (10, 11, 16). Homol- ogous DNA annealing is a vital activity during the process of DNA replication, recombination, and repair ...from ... See full document

10

Rescue of Bacteriophage T7 DNA Polymerase of Low Processivity by Suppressor Mutations Affecting Gene 3 Endonuclease

Rescue of Bacteriophage T7 DNA Polymerase of Low Processivity by Suppressor Mutations Affecting Gene 3 Endonuclease

... of T7 phage. The self-sufficient ge- nome organization of bacteriophage T7 and its robust lytic life cycle have made it an attractive model to investigate genetic adaptation under environmental ... See full document

10

Early gene expression in bacteriophage T7. I. In vivo synthesis, inactivation, and translational utilization of early mRNA's.

Early gene expression in bacteriophage T7. I. In vivo synthesis, inactivation, and translational utilization of early mRNA's.

... In vivo rates of gene-specific messenger production cannot be measured directly; however, they can be calculated from equation 2 above once the following four quantities have been determ[r] ... See full document

17

In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.

In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.

... Whereas we have implicated the gene 3 endonuclease, gene 5 polymerase, and gene 6 exonuclease in in vitro recombination, the gene 4 primase has a paradoxical role; it stimulates exonucle[r] ... See full document

7

Evidence for direct involvement of T7 RNA polymerase bacteriophage DNA replication.

Evidence for direct involvement of T7 RNA polymerase bacteriophage DNA replication.

... The experiments scripts are used as primers for DNA synthesis indicated that the inactivation of the gene 1 18, 19, In other cases this has not yet been RNA polymerase caused the cessati[r] ... See full document

6

Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector

Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector

... (containing DNA or RNA) into the host cell via injection, oral administration or particle bombardment ...antigenic gene is incorporated into the plasmid DNA, which will allow its expression in the ... See full document

8

Synthesis of Bacteriophage-Coded Gene Products During Infection of Escherichia coli with Amber Mutants of T3 and T7 Defective in Gene 1

Synthesis of Bacteriophage-Coded Gene Products During Infection of Escherichia coli with Amber Mutants of T3 and T7 Defective in Gene 1

... If the residual synthesis of DNase, lysozyme, DNA polymerase, and SBP in gene 1 amber mutants of T7 was indeed due to read-through of the host RNA polymerase past the terminator tl rathe[r] ... See full document

8

A unique loop in the DNA-binding crevice of bacteriophage T7 DNA polymerase influences primer utilization

A unique loop in the DNA-binding crevice of bacteriophage T7 DNA polymerase influences primer utilization

... the role of the 401–404 loop by generating and characterizing an altered T7 DNA polymerase in which the 4-aa loop has been ...affect T7 phage growth. This finding is not surprising as ... See full document

7

Genetic analysis of gene 1.2 of bacteriophage T7: isolation of a mutant of Escherichia coli unable to support the growth of T7 gene 1.2 mutants.

Genetic analysis of gene 1.2 of bacteriophage T7: isolation of a mutant of Escherichia coli unable to support the growth of T7 gene 1.2 mutants.

... The products of at least six genes of phage T7 are essential for T7 DNA replication: gene 1 RNA polymerase, gene 2 inhibitor of Escherichia coli RNA polymerase, gene 3 endonuclease, gene[r] ... See full document

9

A Complex of the Bacteriophage T7 Primase-Helicase and DNA Polymerase Directs Primer Utilization

A Complex of the Bacteriophage T7 Primase-Helicase and DNA Polymerase Directs Primer Utilization

... of T7 DNA polymerase and gp4 ⌬ D2D3 bound to a primed DNA template can be assembled un- der conditions that block extension of the primer strand by the ...by T7 DNA polymer- ase. ... See full document

14

A Mutation in the Gene-encoding Bacteriophage T7 DNA Polymerase That Renders the Phage Temperature-sensitive

A Mutation in the Gene-encoding Bacteriophage T7 DNA Polymerase That Renders the Phage Temperature-sensitive

... mutant gene 5 protein when it is synthesized at the non-permissive ...Since DNA polymerase activity in such extracts using salmon sperm DNA as a template is dependent only on T7 DNA po- ... See full document

11

Role of Gene 52 in Bacteriophage T4 DNA Synthesis

Role of Gene 52 in Bacteriophage T4 DNA Synthesis

... Since it has been proposed that a nucleolytic activity is involved in initiation of T4 DNA synthesis 11, 12, 17, 35 and since there is some previous evidence for parental strand nicking [r] ... See full document

10

Characterization of the defects in bacteriophage T7 DNA synthesis during growth in the Escherichia coli mutant tsnB.

Characterization of the defects in bacteriophage T7 DNA synthesis during growth in the Escherichia coli mutant tsnB.

... Our observation that the defects which occur in phage DNA synthesis during infection of tsnB cells closely resemble those which occur during infection of a wild-type cell with a T7 gene [r] ... See full document

9

Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.

... More recently, the nonionic detergent Brij-58 was shown to produce a similarly fast-sedimenting structure, which in electron micrographs appeared to consist of up to 30 phage equivalents[r] ... See full document

12

Role of Gene 46 in Bacteriophage T4 Deoxyribonucleic Acid Synthesis

Role of Gene 46 in Bacteriophage T4 Deoxyribonucleic Acid Synthesis

... 142 Downloaded from http://jvi.asm.org/ on November 11, 2019 by guest In an attempt to establish whether Escherichia coli B infected with N130 an amber mutant defective in gene 46 is rec[r] ... See full document

12

Electrophoresis of bacteriophage T7 and T7 capsids in agarose gels.

Electrophoresis of bacteriophage T7 and T7 capsids in agarose gels.

... Tris/Mg buffer is 0.2 resembling the envelope of capsid II in the elec- M NaCl-0.01 M Tris-chloride pH 7.4-0.001 M MgCl2; tron microscope have been isolated from tem- for the purificatio[r] ... See full document

12

Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA

Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA

... The positions of promoter and terminator sites for the T7-specific RNA polymerase were estimated from the molecular weights of transcripts from exonucleolytically shortened templates 7; [r] ... See full document

13

Roles of the Early Genes of Bacteriophage T7 in Shutoff of Host Macromolecular Synthesis

Roles of the Early Genes of Bacteriophage T7 in Shutoff of Host Macromolecular Synthesis

... The nomenclature used to describe all mutants is that of Studier 35, 37, 38; deletion mutants are identified by their code number, whereas amber mutations are 543 Downloaded from http://[r] ... See full document

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