[PDF] Top 20 A sensitive, type specific, fluorogenic probe assay for detection of human papillomavirus DNA
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A sensitive, type specific, fluorogenic probe assay for detection of human papillomavirus DNA
... contain specific genotypes of human papillomavirus (HPV) DNA, the high-risk ...HPV type 16 (HPV-16), HPV- 18, HPV-31, HPV-33, and HPV-35 ...improve detection of precancerous ... See full document
6
Evaluation of a Novel Highly Sensitive, Broad Spectrum PCR Reverse Hybridization Assay for Detection and Identification of Beta Papillomavirus DNA
... genotype specific, while the four other probes are used in pattern recognition of ...and probe selection is ...beta-PV detection. These general probes were developed for the detection of at ... See full document
9
Nonisotopic Detection and Typing of Human Papillomavirus DNA in Genital Samples by the Line Blot Assay
... HPV DNA detection, detection of high-risk HPV types as a group, and typing of HPV when coamplification was not ...blot assay was not as sensitive as the standard consensus PCR ... See full document
6
Detection of Human Papillomavirus Type 16-Specific T Lymphocytes by a Recombinant Vaccinia Virus-Based Enzyme-Linked Immunospot Assay
... (ELISPOT) assay is a sen- sitive and quantitative assay that has been widely used to detect antigen-specific cytokine-secreting T cells at the single-cell ...ELISPOT assay has been shown to be ... See full document
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Agreement between the AMPLICOR Human Papillomavirus Test and the Hybrid Capture 2 Assay in Detection of High Risk Human Papillomavirus and Diagnosis of Biopsy Confirmed High Grade Cervical Disease
... the assay can be performed on small aliquots of samples in liquid cytology media or on samples obtained from archival paraffin-embed- ded tissue, and it is extremely sensitive (detects ⬍ 100 copies of HPV ... See full document
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Detection of human papillomavirus DNA in cervical lavage specimens by a nonisotopic consensus PCR assay
... RNA probe. Nested sets of primers, syn- thesized by the Regional DNA Laboratory (University of Calgary, Calgary, Alberta, Canada) were used to amplify separately HPV plasmids for types 6, 11, 16, 18, 31, ... See full document
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Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma
... HPV detection in potentially malignant and malignant oral squamous cell carcinoma showed many discrepan- ...[10]. Specific viral genes (E6 and E7) from HPV types 16, 18, and 33 act as oncogenes ... See full document
10
Monitoring liver damage using hepatocyte-specific methylation markers in cell-free circulating DNA
... highly specific, sensitive, and quantitative assay for the detection of human hepatocyte cfDNA, reflecting hepatocyte death in healthy individuals as well as patients with liver ...of ... See full document
10
Nonisotopic Detection of Human Papillomavirus DNA in Clinical Specimens Using a Consensus PCR and a Generic Probe Mix in an Enzyme Linked Immunosorbent Assay Format
... more sensitive than the MY09-MY11 primer system, since it detected more HPV positives than either of the two MY09- MY11 ...for detection of PCR products were identical in the California laboratory and ... See full document
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Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma
... Using HPV 16 E7 primers, PCR products were detected in 51% of sections, whereas HPV 18 E7 specific sequences were detected in 9% of sections. These fre- quencies correspond with Zur-Hausen et al. who reported that ... See full document
5
Rapid and Sensitive Oligonucleotide Ligation Assay for Detection of Mutations in Human Immunodeficiency Virus Type 1 Associated with High Level Resistance to Protease Inhibitors
... A sensitive, specific, and high-throughput oligonucleotide ligation assay (OLA) for the detection of genotypic human immunodeficiency virus type 1 (HIV-1) resistance to Food and ... See full document
7
Development of a Sensitive and Specific Assay Combining Multiplex PCR and DNA Microarray Primer Extension To Detect High Risk Mucosal Human Papillomavirus Types
... sion assay for detection of the different HPV ...amplified DNA fragments are hybridized to the chip, which is followed by an extension reaction in the presence of ddNTPs that fluoresce differently ... See full document
7
Rapid and Sensitive Detection of Physical Status of Human Papillomavirus Type 16 DNA by Quantitative Real Time PCR
... of papillomavirus oncogene transcripts ...HPV DNA (2, 25). The amplification of papillomavirus oncogene transcripts assay is a useful technique based on RT-PCR for analysis of the physical ... See full document
5
MassARRAY Spectrometry Is More Sensitive than PreTect HPV Proofer and Consensus PCR for Type Specific Detection of High Risk Oncogenic Human Papillomavirus Genotypes in Cervical Cancer
... the DNA isolations were quantified and found to have a sufficient concentration of template DNA per the manufacturer’s ...HPV type, and this excluded any possibility of contamination across the group ... See full document
8
Development and Clinical Evaluation of a Highly Sensitive PCR Reverse Hybridization Line Probe Assay for Detection and Identification of Anogenital Human Papillomavirus
... Reverse hybridization by the LiPA. Genotype-specific probes were used to develop a line probe assay (LiPA), the INNO-LiPA HPV prototype research genotyping assay. The oligonucleotide probes ... See full document
10
Evaluation of a Commercialized In Situ Hybridization Assay for Detecting Human Papillomavirus DNA in Tissue Specimens from Patients with Cervical Intraepithelial Neoplasia and Cervical Carcinoma
... (ISH) assay for detecting human papillomavirus (HPV) DNA, we compared the ability of a new ISH probe, Inform HPV III (Ventana Medical Systems, Tucson, AZ), to that of PCR assays to ... See full document
7
Performance of the Aptima High Risk Human Papillomavirus mRNA Assay in a Referral Population in Comparison with Hybrid Capture 2 and Cytology
... AHPV assay at Gen-Probe Incorporated (San Diego, CA). The AHPV assay and the HC2 test are, respectively, nucleic acid amplification and signal amplification tests that allow the qualitative detec- ... See full document
6
A Fluorogenic Probe for Ultrafast and Reversible Detection of Formaldehyde in Neurovascular Tissues
... emissive 7-(diethylamino) coumarin, PFM was almost non-fluorescent in PBS, presumably due to the substitution of the less electron-withdrawing hydrazonate group which decreased the electronic push-pull effect. However, ... See full document
9
Induction of Human Papillomavirus-Specific CD4+ and CD8+ Lymphocytes by E7-Pulsed Autologous Dendritic Cells in Patients with Human Papillomavirus Type 16- and 18-Positive Cervical Cancer
... responses from the same patients. We chose the E7 oncopro- tein of HPV 16 and HPV 18 as a target antigen for the fol- lowing reasons: (i) HPV 16 and HPV 18 are associated with the vast majority of cervical cancer, and ... See full document
9
Optimization of a Human Papillomavirus-Specific Enzyme-Linked Immunosorbent Assay
... coating solution versus 0.049 to 0.729 for carbonate buffer coating solution. This difference in observed intensity may reflect a denaturing effect of the VLP’s conformational epitope due to the use of carbonate buffer. ... See full document
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