[PDF] Top 20 Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's.

... BARKLIE CLEMENTS* Department of Virology and Medical Research Council Virology Unit, University of Glasgow, Church Street, Glasgow Gll 5JR, Scotland The locations on the genome of transc[r] ... See full document

... during early HSV-1 infection (see also reference ...Sp100 mRNA in different cells, we show that untreated HEp-2 cells express predominantly the Sp100A isoform and IFN- ␤ treatment leads to increased ... See full document

... During herpes simplex virus type 1 (HSV-1) latent infection in human dorsal root ganglia, limited viral transcription, which has been linked to HSV-1 reactivation ability, ... See full document

... and virus particles were pelleted by centrifugation (23,000 ⫻ g for 2 h at ...in 1 ml of modified 199 medium (without phenol red and FCS) and layered onto 35-ml preformed gradients of 5 to 15% Ficoll 400 ... See full document

... this virus lacks ICP0 and encodes a temperature-sensitive ...parental virus were confirmed by Southern hybridization analysis of viral DNA, as described previously ...the virus stocks used were 32 ... See full document

... We used hybrid-arrested translation to demonstrate that the 155,000-dalton polypeptide encoded by the 6-kb mRNA mapping in HindIII fragment J 0.181 to 0.259 is the only species of that s[r] ... See full document

... In the present study, we focused on the role of one HSV-1 IE protein, ICP22, a 420-residue protein encoded by the Us1 gene, conserved in the subfamily Alphaherpesvirinae of the family Her- pesviridae. It was ... See full document

... varicella-zoster virus (human herpesvirus 3), another alphaher- pesvirus from the Varicellovirus genus, lacks a “canonical” SIAH-1 interaction motif, as do the betaherpesviruses human cytomegalovirus and ... See full document

... Cytoplasmic IE RNA was hybridized to 260-b fragment resulted from hybridization of either a 3', 32P-labeled SalB5 or a 5', 32P-labeled B4RJ HSV-1 DNA probe, and RNA/DNA hybrids the B4R1 [r] ... See full document

... We isolated specific HSV-1 mRNA species mapping within the region of interest by hybridization of late polyA mRNA to the appropriate restriction fragment bound to cellulose see above.. S[r] ... See full document

... was found to react strongly with a 175 kDa protein species in these experiments (Fig. 2). The molecular mass of this large polypeptide suggested that it might be the 175 kDa HSV-1 immediate early ... See full document

... Cells infected by viruses expressing either EG110 or Vmw110 are blocked between prophase and metaphase stages of mitosis. To analyze in detail the effect of infection on syn- chronized cells, fluorescence microscopy was ... See full document

... wild-type virus, 22/n199, and 22/n199R in Vero, RAB-9, HEL, and AGMK ...wild-type virus, 22/n199, or 22/n199R at an MOI of ...of virus was determined by plaque assay on Vero cell ... See full document

... Hsc70, we considered whether ICP22 and Hsc70 might physi- cally interact. To test this, we carried out a coimmunoprecipi- tation experiment. HeLa cells were mock infected or infected with WT HSV-1, TF22, or the ... See full document

... VP16 mRNA, but not its nuclear export, are reminiscent of several recent studies that have analyzed how the process of splicing enhances gene expression (38, 49, 50, ...an mRNA can dramatically boost the ... See full document

... Isolation and characterisation of a herpes simplex virus type 1 mutant containing a deletion within the gene encoding the immediate early polypeptide VmwllO.. The DNA-binding domains of [r] ... See full document

... with virus lacking the VP22 gene, ICP0 failed to localize to these puncta to any degree ...the virus at Wt levels without exhibiting steady-state localization to these cytoplasmic puncta, as indicated by ... See full document

... a require- ment for coactivators in the activity of VP16 on IE gene ex- pression, we repeated the low-MOI experiments in the pres- FIG. 2. HSV-1 IE gene expression is not impaired in SiHa cells but is augmented ... See full document

... HSV type 1 (HSV-1) induces expression of the Oct 1 gene in sensory neurons ...the separation of the role of the host protein from that of viral proteins or the evaluation of variations ... See full document

... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document