[PDF] Top 20 Herpes Simplex Virus 1 Glycoprotein M and the Membrane-Associated Protein UL11 Are Required for Virus-Induced Cell Fusion and Efficient Virus Entry
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Herpes Simplex Virus 1 Glycoprotein M and the Membrane-Associated Protein UL11 Are Required for Virus-Induced Cell Fusion and Efficient Virus Entry
... and UL11 are required for virus-induced cell fusion and efficient virus entry, most likely because they directly or indirectly interact with the viral ... See full document
9
Extended Synaptotagmin 1 Interacts with Herpes Simplex Virus 1 Glycoprotein M and Negatively Modulates Virus-Induced Membrane Fusion
... and Cell Biology, University of Montreal, Montreal, Québec, Canada b Institute for Research in Immunology and Cancer, Department of Chemistry, University of Montreal, Montreal, Québec, Canada ABSTRACT Enveloped ... See full document
19
Vaccinia Virus L1 Protein Is Required for Cell Entry and Membrane Fusion
... an entry/fusion complex (EFC) comprised of at least eight viral proteins (A16, A21, A28, G3, G9, H2, J5, and L5) that together with an associated protein (F9) participates in entry of ... See full document
8
Interaction and Interdependent Packaging of Tegument Protein UL11 and Glycoprotein E of Herpes Simplex Virus
... The UL11 tegument protein of herpes simplex virus plays a critical role in the secondary envelopment; however, the mechanistic details remain ...of UL11 in the budding process in ... See full document
10
The Herpes Simplex Virus Type 1 UL20 Protein Modulates Membrane Fusion Events during Cytoplasmic Virion Morphogenesis and Virus-Induced Cell Fusion
... UL20 protein is required in transient expression assays for the Golgi- dependent glycosylation and cell surface expression of gK, implying a functional association between the UL20 protein and ... See full document
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Vaccinia Virus A21 Virion Membrane Protein Is Required for Cell Entry and Fusion
... or entry seemed most likely, as A21 is an IMV membrane ...⫹ virus and wild-type VACV but were barely detectable in cells infected with A21 ⫺ virus ...VACV-induced cell rounding ... See full document
12
Packaging Determinants in the UL11 Tegument Protein of Herpes Simplex Virus Type 1
... The UL11 gene of herpes simplex virus type 1 encodes a 96-amino-acid tegument protein that is myristylated, palmitylated, and phosphorylated and is found on the cytoplasmic faces ... See full document
8
UL36p Is Required for Efficient Transport of Membrane-Associated Herpes Simplex Virus Type 1 along Microtubules
... a membrane-associated HSV-1 particle forms which has greatly reduced infectivity and a decreased ability to both bind and traffic along ...cular-shaped membrane with a dense layer of smooth ... See full document
7
Mutations in Herpes Simplex Virus Glycoprotein D Distinguish Entry of Free Virus from Cell-Cell Spread
... Probe membrane (Bio-Rad), and probed with sequences corresponding to the Southern probe shown in ...the protein-coding sequence of neighboring genes, gJ and ...vRR1097, virus stocks obtained 3 to 5 ... See full document
10
Herpes Simplex Virus Glycoprotein B Binds to Cell Surfaces Independently of Heparan Sulfate and Blocks Virus Entry
... for entry, association of the gB receptor with these structures may be ...that virus entry into some cell types occurs by endocytosis rather than by direct fusion (40, ...is ... See full document
10
Pseudorabies Virus Glycoprotein M Inhibits Membrane Fusion
... investigate membrane fusion mediated by pseudo- rabies virus (PrV) ...immediate-early 1 promoter-enhancer of human cytomegalovirus were transfected into rabbit kidney cells, and the extent of ... See full document
9
The Amino Terminus of Herpes Simplex Virus Type 1 Glycoprotein K (gK) Modulates gB-Mediated Virus-Induced Cell Fusion and Virion Egress
... through fusion with the outer nuclear lamel- ...to cell surfaces, presumably following the cellular secretory pathway (33, 47, ...in virus-induced cell fusion, gK and UL20 are ... See full document
13
Genetic Analysis of the Herpes Simplex Virus Type 1 UL20 Protein Domains Involved in Cytoplasmic Virion Envelopment and Virus-Induced Cell Fusion
... and membrane topol- ogy of ...infectious virus production and virus-induced cell ...in protein-protein interactions necessary for UL20p-associated viral ... See full document
15
Dual Split Protein-Based Fusion Assay Reveals that Mutations to Herpes Simplex Virus (HSV) Glycoprotein gB Alter the Kinetics of Cell-Cell Fusion Induced by HSV Entry Glycoproteins
... of virus entry (31, 33) and others that resembled the WT in endpoint fusion ...of fusion. Finally, we exam- ined the rate of fusion induced by a double mutant with alterations in ... See full document
14
Pseudotyping of Glycoprotein D-Deficient Herpes Simplex Virus Type 1 with Vesicular Stomatitis Virus Glycoprotein G Enables Mutant Virus Attachment and Entry
... this protein has been used with considerable success for pseudotyping other viruses (1, 43, 52, 59, 62) and mediates both attachment and penetration of VSV (15, 41, ...VSV entry involves ... See full document
8
The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment
... included: 1) cluster-to-alanine mutants in which a cluster of proximal amino acids were changed to alanine residues; 2) single amino acid replace- ment mutants within alanine cluster regions; 3) carboxyl terminal ... See full document
12
Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion.
... To determine whether gB is essential for cell fusion, a gB-null syncytial virus (synO82) was isolated; synO82 caused extensive fusion on the gB-transformed Vero cells (D6) but not on Ver[r] ... See full document
9
The UL45 gene product is required for herpes simplex virus type 1 glycoprotein B-induced fusion.
... To determine whether this mutation was acquired in the newly syncytial viruses (C4 series) derived from cotrans- fection of the Fl syn mutation into 17syn+ or, conversely, lost in the no[r] ... See full document
10
HveA (Herpesvirus Entry Mediator A), a Coreceptor for Herpes Simplex Virus Entry, also Participates in Virus-Induced Cell Fusion
... The mutant KOS-SR1 is a Syn recombinant isolated from the coinfection of Vero cells with KOS-804 and KOS-Rid1 and carries the mutant gK and gD alleles of the parental strains. Vero cells were coinfected with 10 PFU each ... See full document
9
Regulation of Herpes Simplex Virus Glycoprotein-Induced Cascade of Events Governing Cell-Cell Fusion
... IMPORTANCE Cell-cell fusion mediated by HSV glycoproteins requires gD, gH/gL, gB, and a gD ...that fusion by wild- type HSV-2 glycoproteins occurs twice as fast as that achieved by ... See full document
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