[PDF] Top 20 Simplified acetylcysteine alkali digestion decontamination procedure for isolation of mycobacteria from clinical specimens
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Simplified acetylcysteine alkali digestion decontamination procedure for isolation of mycobacteria from clinical specimens
... The total alkali exposure time in the modified NALC-NaOH method should be kept to a minimum; if necessary or feasible, the concentration of NaOH in the digestion solution may be reduced [r] ... See full document
5
Isolation and characterization of Non tuberculous Mycobacteria from clinical specimens
... the clinical laboratory has relied on growth characteristics, pigmentation, and selected biochemical ...the specimens; yet, Deoxyribonucleic acid/ Ribonucleic acid probes would frequently able to identify ... See full document
138
Evaluation of the MB/BacT system and comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens
... all mycobacteria isolated) incubated for 6 weeks compared to that of the BACTEC 460 culture was similar to that of other egg-based media used in other evalu- ations (1, ...Stonebrink from the 6th week to ... See full document
5
Identification of non tuberculous mycobacteria isolated from clinical specimens at a tertiary care hospital: a cross sectional study
... the clinical significance of an NTM iso- late is vital for establishing its role in causing patient’s ...symptoms. Clinical information is routinely collected at AKUH Clinical Microbiology Laboratory ... See full document
7
Recovery of Mycobacteria from Clinical Specimens and Assessing Drug Susceptibility Test of Mycobacterium Tuberculosis Specimens by MGIT
... in clinical specimens can lead to effective intervention ...publications from Center for Disease Control (CDC) and Prevention (Atlanta, GA) have recommended turnaround times extending up to 21 days ... See full document
9
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... Two bottles of MB/BACT (procured from Biomerieux) were taken, each of which was inoculated with 500µl of the decontaminated deposit aseptically. The date of the inoculation was noted. The tubes were incubated at ... See full document
6
Sequence capture PCR improves detection of mycobacterial DNA in clinical specimens
... DNA from as few as 10 mycobacteria in 750 m g of total DNA, and the sensitivities of these two systems for the detection of mycobacterial DNA in tuberculous effusions were not ...in clinical ... See full document
7
Molecular Identification and Susceptibility of Trichosporon Species Isolated from Clinical Specimens in Qatar: Isolation of Trichosporon dohaense Taj Aldeen, Meis & Boekhout sp nov
... associated clinical symptoms such as pyuria, suggested urinary tract ...isolated from specimens that did not meet such criteria were excluded from the ...isolated from nails were ... See full document
9
Prevalence of Nontuberculous Mycobacteria (NTM) in Iranian Clinical Specimens: Systematic Review and Meta-Analysis
... Overall, this meta-analysis indicated that prevalence of NTM in Iranian clinical specimens was 1.3% (1.1-1.5%). Based on the subgroups analysis was observed that the prevalence of NTM was higher when ... See full document
12
Comparison of two automated DNA amplification systems with a manual one tube nested PCR assay for diagnosis of pulmonary tuberculosis
... resulting from the clinical evalu- ...of mycobacteria and neutraliza- tion of inhibitors in different ...B specimens negative by nPCR are analyzed. When the extracts left over from ... See full document
5
Evaluation of Various Culture Media for Detection of Rapidly Growing Mycobacteria from Patients with Cystic Fibrosis
... methods, from sputum samples from patients with CF was used for evaluation of all ...obtained from the microbiology department of Freeman Hospital (Newcastle upon Tyne, United Kingdom), and all were ... See full document
7
An optimized PCR leads to rapid and highly sensitive detection of Borrelia burgdorferi in patients with Lyme borreliosis
... most clinical samples tested than using the ospA ...urine specimens from patients with Lyme arthritis, in 27% of urine specimens from patients with neuroborreliosis, in 72% of SF ... See full document
6
Direct Drug Susceptibility Testing of Mycobacterium tuberculosis for Rapid Detection of Multidrug Resistance Using the Bactec MGIT 960 System: a Multicenter Study
... imens are needed. Some of the noncommercially available direct tests include the nitrate reductase assay (NRA) and microscopic observation drug susceptibility (MODS) assay. These tests have been developed as “in house” ... See full document
6
Engineered BGMK Cells for Sensitive and Rapid Detection of Enteroviruses
... Cells. BGMK and BGMK-hDAF cells were prepared in-house and seeded in 48-well plates. Primary rhesus monkey kidney (pRhMK) cells and MRC-5 cells were obtained from ViroMed Inc. (Minneapolis, Minn.) in 48-well ... See full document
6
Sensitive chemiluminescent enzyme linked immunosorbent assay for quantification of human immunoglobulin G and detection of herpes simplex virus
... Comparison of virus isolation in cell culture and assay by absorptiometric ELISA and CELISA for the detection of HSV in clinical specimens No.. Source of specimen.[r] ... See full document
5
Do Staphylococcus epidermidis Genetic Clusters Predict Isolation Sources?
... comes from the selec- tion of contaminant isolates from infected patients using colony morphology as the ...and clinical assessments (8, 48), SNP-based characterization of two or more isolates ... See full document
9
Insights into the hierarchical structure and digestion rate of alkali modulated starches with different amylose contents
... necessary to understand how alkali treatment changes the structure and digestion behavior of starch... Alkali treatment has been shown capable of altering the structures morphologyCai et[r] ... See full document
42
Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens
... On plates containing the smallest inoculum, there was no growth on BCAP medium after 146 h of incubation, whereas there were 1 and 3 CFU on BMPA and BCYEa media, respectively, after 50 h[r] ... See full document
6
Geographic and Temporal Trends in Isolation and Antifungal Susceptibility of Candida parapsilosis: a Global Assessment from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to 2005
... clear from this survey that de- creased susceptibility may occur in certain geographic regions and in select institutions (10, 26, 51, 57), suggesting that mon- itoring of local susceptibility profiles may be ... See full document
8
Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens
... Differential agar medium A7 for identification of Ureaplasma urealyticum human T mycoplasmas in primary cultures of clinical material.. Ureaplasma urealyticum gen.[r] ... See full document
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