[PDF] Top 20 Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
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Single-Chain Antibody Fragment Specific for Plasmodium vivax Duffy Binding Protein
... Recombinant PvRII molecules. Recombinant PvRII (C4-to-C7 cysteine-rich region of PvDBP II) protein of SK-1 strain isolated in South Korea was produced using a previously reported method (17). Briefly, the PvRII ... See full document
6
Easy and fast method for expression and native extraction of Plasmodium vivax Duffy binding protein fragments
... Valencia, CA, USA) pre-equilibrated with NEB or DEB for purification by solid-phase affinity chromatography. The resin-protein mixture was then poured onto a chro- matography column and washed with 20 mL buffer ... See full document
9
Extracellular vesicles expressing a single-chain variable fragment of an HIV-1 specific antibody selectively target Env+ tissues
... to antibody staining. (E) Binding ability of exosomes to recombinant HIV-1 Env were ...the binding between exos and Env- conjugated beads, in order to proof the specificity of binding mediated ... See full document
15
Design and Immunogenicity of a Novel Synthetic Antigen Based on the Ligand Domain of the Plasmodium vivax Duffy Binding Protein
... bind Duffy-positive human erythrocytes in an erythrocyte-binding assay similar to that previously described (8, 19, 31, 34, ...42). Duffy-positive erythrocytes treated with chymotrypsin were used as ... See full document
7
Genetic diversity, natural selection and haplotype grouping of Plasmodium vivax Duffy binding protein genes from eastern and western Myanmar borders
... the Plasmodium merozoite and the host red blood cell (RBC) is central to the invasion process of the ...P. vivax involves the Duffy-binding protein (PvDBP), a microneme protein, ... See full document
14
Immunogenicity of a Synthetic Vaccine Based on Plasmodium vivax Duffy Binding Protein Region II
... within the different alleles. No significant difference in inhibition was observed for any antibody group against the Sal1, 27.16, and P alleles. The 7.18 allele was the most refractory to inhibition, fol- lowed ... See full document
9
A Novel Erythrocyte Binding Protein of Plasmodium vivax Suggests an Alternate Invasion Pathway into Duffy Positive Reticulocytes
... polyclonal antibody showed very negligible reactivity with the denatured antigen com- pared with the refolded antigen, thus confirming the presence of con- formational epitopes in the refolded antigen ... See full document
5
Transient and Stable Expression of Single-chain Antibody Fragment scFv13R4 in Chinese Hamster Ovary Cells.
... the protein of interest stably is a long and laborious ...heterologous protein to be expressed, the DNA must be integrated into the euchromatin, which is loosely coiled and transcriptionally ...to ... See full document
96
Natural selection and genetic diversity of domain I of Plasmodium falciparum apical membrane antigen 1 on Bioko Island
... One hundred thirty-one different haplotypes of DI were identified in 214 Bioko P. falciparum isolates. Compari- son of 214 DI sequences with PfAMA-1 sequences from the 3D7 P. falciparum clone (GenBank Accession Num- ber ... See full document
16
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
... a Duffy positive phenotype was used, given that PvGAMA reportedly has a binding role regardless of such antigen’s expression ...reticulocyte binding as P. vivax merozoites have tropism for ... See full document
11
The Antiviral Mechanism of an Influenza A Virus Nucleoprotein Specific Single Domain Antibody Fragment
... VHH binding to vRNPs prevents importin binding to ...4 Binding interface of NP and ␣ ...VHH binding are shown in ...enhances binding to importin- ␣ is shown in ...nucleozin ... See full document
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Adenovirus-mediated anti-AEG-1 ScFv expression driven by stathmin promoter inhibits tumor growth in cervical cancer
... The protein concentrations were deter- mined using the Bio-Rad protein assay kit (Bio-Rad, CA, ...total protein) was then analyzed by SDS-PAGE and Western ...Ƙ chain antibody), cleaved ... See full document
9
Plasmodium vivax infection in Anajás, State of Pará: no differential resistance profile among Duffy negative and Duffy positive individuals
... The Duffy blood group locus, at position q21–q25 on chromosome 1 [7], is characterized by three main alleles: FY * A , FY * B and FY * B ES ...a single amino acid difference and gives the common Fy (a+b-), ... See full document
6
Temporal changes in Plasmodium falciparum reticulocyte binding protein homolog 2b (PfRh2b) in Senegal and The Gambia
... [3]. Plasmodium falciparum Reticulocyte binding protein homologues (PfRhs) are expressed at the apical surface of invasive merozoite [4–7] and are believed to play a role in the recognition of the ... See full document
11
Asymptomatic Plasmodium vivax infections among Duffy-negative population in Kedougou, Senegal
... IgG antibody responses to P. vivax MSP1 antigen with the same samples ...P. vivax in- fections in children C8 and C29 over a period of 2 years might be due to persistent ...P. vivax infections ... See full document
5
Distinct genetic difference between the Duffy binding protein (PkDBPαII) of Plasmodium knowlesi clinical isolates from North Borneo and Peninsular Malaysia
... asite’s protein and its corresponding receptor on the sur- face of the ...erythrocyte. Plasmodium knowlesi uses the Duffy blood group antigen as a receptor to invade eryth- rocytes ...The ... See full document
7
Diversity pattern of Duffy binding protein sequence among Duffy negatives and Duffy positives in Sudan
... P. vivax population and this information could be used for the rational design of a PvDBP-based multi-allele ...P. vivax isolates (Additional file ...P. vivax population and further studies with a ... See full document
10
Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax
... malaria- specific antibodies play essential roles in acquired immu- nity via the phagocytosis and opsonization of parasites [3], and the importance of immunity is evident in experi- mental animal models as well as ... See full document
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Genetic diversity, haplotypes and allele groups of Duffy binding protein (PkDBPαII) of Plasmodium knowlesi clinical isolates from Peninsular Malaysia
... The PkDBPαII (K = 11.736; Hd = 0.986; π = 0.01274) in Peninsular Malaysia seemed to be more diverse than P. vivax PvDBPII in some neighbour countries, such as Myanmar (K = 7.851; Hd = 0.875; π = 0.00790), Korea (K ... See full document
7
Molecular epidemiology of Plasmodium vivax and Plasmodium falciparum malaria among Duffy positive and Duffy negative populations in Ethiopia
... the Duffy antigen. The proportion of Duffy-negatives observed in the clinical samples is similar to an earlier study that reported an average of 20% homozygous Duffy-negative patients in Harar and ... See full document
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