[PDF] Top 20 Stochastic NANOG fluctuations allow mouse embryonic stem cells to explore pluripotency

... of NANOG expression, unperturbed by intrinsic noise (Silva et ...of pluripotency (Silva and Smith, 2008; Nichols and Smith, 2009), in which gene expression heterogeneities would be abolished, and all mESCs ... See full document

... feeder cells was lower than 4 ...Feeder cells did not only secreted soluble factors, but also provided extracellular matrix and cellular contact to the ...feeder cells would be shoved. Thus, the ... See full document

... the stem cell fate is a prerequisite in our pursuit to control the stem cell ...governing mouse ES cell fate ...liver cells that could sustain self-renewal of mouse ES cells in ... See full document

... the cells were incubated in 3% bovine serum albumin at RT for 2 h to block nonspecific ...the cells were washed twice with PBS and incubated with secondary antibodies for 1 h in the ...The cells were ... See full document

... dead cells. In a 96-well plate, 10,000 cells/well were seeded and stored for 24 hours in a CO 2 incubator, to stimulate cell ...The cells were then incubated with 50 µL of 2 mg/mL MTT solved in PBS, ... See full document

... Sequence encoding Cas9, murine Oct4, Sox2, Ddx56, and EGFP were cloned into pENTR. Ddx56 domain truncations were generated by overlap PCR on pENTR- Ddx56. Cas9, Oct4, Sox2, EGFP, Ddx56, and its domain truncations were ... See full document

... include pluripotency, self-renewal and similar gene expression ...stringent pluripotency assay, tetraploid embryo complementation, demonstrated that mouse iPSCs can give rise to all tissues of the ... See full document

... whole mouse organism and not only the ...another mouse model holding a different ...Sox17iCre mouse line was used, which was kindly provided by ...of Stem Cell Research, Helmholtz center, ... See full document

... Furthermore, NSCs containing NPs were maintained under sterile culture conditions for 10 days and subjected to TEM and NMR relaxation studies over this period to evalu- ate NP retention. TEM showed that uptaken particles ... See full document

... Pluripotency markers in equine embryos 217 218 Since early equine embryogenesis differs significantly to that of the mouse, pig, cow and 219 human [34,53], it is reasonable to anticipate[r] ... See full document

... pluripotent cells is detected simply because of the iP? The studies described in [32,34] did not investigate the role of the iP complex in maintain- ing ... See full document

... Finally, we used this myelin formation assay to image the myelination process in real time, following oligodendrocytes over several days as they surveyed around and along axons by constantly extending and retracting ... See full document

... the mouse, such as derivation of haploid mouse embryos by different strategies ...of mouse embryonic stem (ES) cells shed light on haploid cell derivation in the mouse ... See full document

... in embryonic stem cells (ESCs) led to a reduction of H3K4me3 levels particularly at promoters of highly transcribed genes (Clouaire et ...express pluripotency markers (Carlone et ...in ... See full document

... Cell lysate of mouse ESCs in Co-IP buffer containing 10 mM Hepes (pH 7.6), 250 mM sodium chloride, 0.1% Nonidet P-40, 5 mM EDTA, 1 mM phenylmetha- nesulfonyl fluoride and 1 mM sodium fluoride was col- lected and ... See full document

... Sox2, Nanog, ...Interestingly, pluripotency transcription fac- tors mentioned above directly control the expression of the cluster by co-occupying its promoters or ...way, pluripotency factors, the ... See full document

... (XY) cells. Thus, to equalize X-encoded gene expression, female somatic cells transcriptionally silence one of the two X chromosomes in a process named X chromosome inactivation (XCI), which leads to ... See full document

... (OSE) cells were proven to undergo an epithelial-mesenchymal transition in vitro, that can be reversed under proper stimuli ...of cells are present in the cortical zone: i) epithelial cells of ... See full document

... chicken embryonic stem cell mRNA was used with P06GFPEcoRIS (5 ⬘ -GGGAATTCGCATGTAAAAGCCAAA-3 ⬘ ) and P06GFPKpnIAS (5 ⬘ -ATGGTACCTCAGTGGCTGCTGTTGT-3 ⬘ ) primers to amplify the cPouV coding ...and ... See full document

... true stem cells in agriculture and how can we conquer it? The answer might lie in the fact that there is a basic difference in the development of embryo within different mammalian species (Nichols & ... See full document