[PDF] Top 20 Stochastic patterning in the mouse pre implantation embryo

... In a second patterning phase, a definite relationship with blastocyst morphology is established. This could be achieved in two ways. First, the emerging molecular signature defines the properties of the cells and ... See full document

... the embryo with iron to allow for successful neural tube closure, implying that supplementation with both folic acid and iron prior to conception may reduce the incidence of NTD- affected pregnancies even ... See full document

... However, the N-terminal Smad2 MH1 domain contains a distinctive thirty amino acid insert predicted to impose steric constraints that selectively disrupt DNA binding (Shi et al., 1998). Consistent with this DNA-binding ... See full document

... mammalian embryo communicate with each other and their extra-embryonic ...2003). PrE cells in contact with the ICM subsequently become visceral endoderm (VE) and act as a substrate for the remaining ... See full document

... indicated. Mouse embryos divide asynchronously and, although it is possible to associate specific morphologies with the time elapsed since mating ...particular embryo (Plusa et ...the embryo, some ... See full document

... the absence of Smad4, PGC number was assayed one day later at E8.5. In wild-type embryos at this stage, AP-positive PGCs (~100) have dispersed from the base of the allantois and intercalated into the hindgut epithelium ... See full document

... (Acampora et al., 1995; Matsuo et al., 1995; Ang et al., 1996). Heterozygous Otx2+/- embryos in an appropriate genetic back- ground show defects of the head such as serious brain abnormalities and craniofacial ... See full document

... during implantation that are critical for the establishment of pregnancy, especially in the well-studied mouse (Cha et ...in mouse causes the blastocyst to implant after the preferred window of ... See full document

... There is evidence that DNA methylation is passively removed both from the paternal and maternal genomes from the first S- phase (one-cell stage) up to the morula/early blastocyst stage (Howlett and Reik, 1991; Kafri et ... See full document

... the mouse also exhibits any relationship between the first cleavage plane and the later body ...stage mouse embryo are equally capable of giving rise to essentially any part of the final body, recent ... See full document

... That mutation in Lamc1 causes such early lethality probably stems from the fact that laminin γ1 is found in 10 out of the 15 known laminin trimers. Thus, removing γ1 effectively prevents assembly of almost all basement ... See full document

... Late-primitive-streak stage (gastrula) embryos were collected from 7.5- day pregnant mice. The ectoplacental cone was isolated and transferred to 50 µl of lysis buffer, incubated for 60 min at 55°C and 10 min at 95°C, ... See full document

... on implantation in the mouse (Huet and Dey, 1990); blastocyst’s state of activity determines the window of implantation (Paria et ...including implantation (Lim et ...the embryo-uterine ... See full document

... the embryo stage or during the derivation process, human ICM cells undergo a lineage restriction, as they resemble mouse post-implantation epiblast cells both in their molecular characteristics and ... See full document

... preimplantation embryo and female reproductive tract is essential for embryo development and ...inhibited embryo development and caused infertility [6, 7, 8, ...normal embryo development ...in ... See full document

... an embryo recovers from experimental ...or embryo that relies on the relative and not the absolute concentration of a ...the embryo itself or its environment can be adopted as spatial cues to ... See full document

... Fig. 1. Klf5 is required for normal mouse pre-implantation development. (A)  Confocal cross- sections showing DIC images (top) and Klf5 (middle) and Draq5 nuclei (bottom) staining. Nuclear Klf5 ... See full document

... Recent studies showed that angiomotin (Amot) is required in inner cells to activate Hippo signaling and phosphorylate YAP (Hirate et al., 2013; Leung and Zernicka-Goetz, 2013). Without Amot and Amotl2, a homolog with ... See full document

... the embryo and the genetic condition; euploid and aneuploid ...compared embryo morphology and rate of aneuploidy, found a weak correlation between variables and concluded that establishing the quality of an ... See full document

... Hysteroscopic embryo delivery provides a visually confirmed technique for embryo ...the embryo(s), which may increase live delivery rate and decrease untoward side effects from embryo ... See full document