Top PDF Studies on the L-Amino Acid Oxidase of Neurospora Crassa

Studies on the L-Amino Acid Oxidase of Neurospora Crassa

Studies on the L-Amino Acid Oxidase of Neurospora Crassa

The Mechanism of The Biotin Effect The Effect of Low Biotin When an L-amino a cid is added to a crude extract of Neurospora mycelium grown on medium containing biotin at 0.251' /liter 1/[r]

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Part I  Synthesis of L amino acid oxidase by a serine  or glycine requiring strain of neurospora  Part II  Studies concerning multiple electrophoretic forms of tyrosinase in neurospora

Part I Synthesis of L amino acid oxidase by a serine or glycine requiring strain of neurospora Part II Studies concerning multiple electrophoretic forms of tyrosinase in neurospora

PARl' I SYNTHESIS OF L AMINO ACID OXIDASE BY A SERINE OR GLYCINE R~RING STRAIN OF NEIJROOPORA PARl' II STUDIES CONCERNING MUIJrIPLE ELEX TROPHORErIC FORMS OF TYROOINASE IN NEIJROOPORA Thesis by Joyce[.]

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Studies on the Metabolism of Threonine and Related Substrates in Neurospora Crassa

Studies on the Metabolism of Threonine and Related Substrates in Neurospora Crassa

threonine. It was concluded that alpha-aminobutyric acid, glutamic acid or a deaminated alpha-ketobutyric acid pre- cursor in equilibrium with alpha-ketobutyric could [r]

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SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. I. GENETIC STUDIES

SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. I. GENETIC STUDIES

Subsequently all seven inde- pendently occurring td,,, sulo, isolates were crossed to selected strains containing linkage group VI1 markers: To select against loss of th[r]

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COMPLEMENTATION STUDIES WITH ISOLEUCINE-VALINE MUTANTS OF NEUROSPORA CRASSA

COMPLEMENTATION STUDIES WITH ISOLEUCINE-VALINE MUTANTS OF NEUROSPORA CRASSA

A total of 616 mutants capable of growth on a medium supplemented with isoleucine and valine, but not on minimal medium, were obtained through the use of nine diff[r]

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SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. II. BIOCHEMICAL STUDIES

SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. II. BIOCHEMICAL STUDIES

The enzymological properties of the suppressed enzyme, i.e., InGP utilizing activities, are so similar to those of the wild-type protein that second- site reversion or[r]

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LINKAGE STUDIES WITH BIOCHEMICAL MUTANTS OF NEUROSPORA CRASSA

LINKAGE STUDIES WITH BIOCHEMICAL MUTANTS OF NEUROSPORA CRASSA

Centromere distance was calculated from all crosses (except those in.. Segregation type 1 represents asci in which sex and both mutant genes segregated in the first division an[r]

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Studies of the biosynthesis of histidine in Neurospora crassa

Studies of the biosynthesis of histidine in Neurospora crassa

used by Ahmed et ale Indeed, if the JA mutant 1964 in the experiments described above, did contain histidinol dehydrogenase which had significantly different heat inactivation properties[r]

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STUDIES OF ADENYLOSUCCINASE IN MUTANTS AND REVERTANTS OF NEUROSPORA CRASSA

STUDIES OF ADENYLOSUCCINASE IN MUTANTS AND REVERTANTS OF NEUROSPORA CRASSA

On the other hand, the primary F12 reverse mutant having 25 percent of wild type enzyme activity gave rise (through secondary mutant Mi ) to secondary revertants possessing [r]

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F-Actin Dynamics in Neurospora crassa

F-Actin Dynamics in Neurospora crassa

Coexpression of Lifeact-TagRFP and ␤ -tubulin–GFP re- vealed distinct but coordinated recruitment of F-actin and mi- crotubules during different stages of cell polarization and tip extension during colony initiation. Previous studies using mi- crotubule-depolymerizing drugs showed that germ tube emer- gence (but not elongation) can be achieved in N. crassa without microtubules (7). Our data showing that polarization of F-actin always preceded polarization of microtubules further rein- forces the notion that germ tube emergence and elongation is a two-step process (9, 21) that first involves F-actin to establish a polarized bud and maintain tip polarity but subsequently requires microtubules for further extension. In contrast, CATs are thinner than germ tubes, show determinate growth (45), and do not require microtubules to facilitate cell fusion (46). Consistently, we observed the dynamic rearrangement of actin organization during CAT formation and fusion, suggesting a predominant role for actin in these processes. This notion is supported by findings in Ustilago maydis, where it has been demonstrated that cell-cell recognition and cell-cell fusion ex- clusively depend on F-actin during all stages of polar growth whereas microtubules are required only for long-distance growth of hyphae (19). The function of CATs is to connect cells that are less than 10 to 12 ␮ m apart, i.e., CATs do not need to extend further than 5 to 6 ␮ m. The F-actin cytoskel- eton is apparently sufficient to support this short-distance growth and to facilitate fusion. Our observations suggest that recruitment of both cytoskeletal elements occurs in a distinct but coordinated manner and might influence which protrusion is being formed and maintained at any point in time.
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Enzyme induction in Neurospora crassa

Enzyme induction in Neurospora crassa

The induction of tyrosinase and L-amino acid oxidase was fully restored when calcium, copper, and amino acids were added to the induction buffer. EDTA inhibition of ind[r]

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Isolation and Characterization of an L Amino Acid  Oxidase Producing Marine Bacterium

Isolation and Characterization of an L Amino Acid Oxidase Producing Marine Bacterium

LAAOs form a family of proteins with various enzymatic properties, structure and biological function. Extensive studies indicate that LAAOs have promising biotechno- logical and medical applications. This enzyme is widely distributed in nature including snake venoms, insect drugs, sea hare, fungi, bacteria and algae. Unlike snake venom LAAOs which have been widely and deeply investigated to show broad bioactivities such as apoptosis, cytotoxic- ity, edema, hemolysis, hemorrhage, platelet aggregation, parasite-killing activity and antimicrobial activity, non- snake venom LAAOs need to be further studied and their functional role and application remain to be revealed. Especially, very little is known about LAAOs from ma- rine microorganism. In this study, we successfully iso- lated an LAAO-producing marine bacterium from inter- tidal zone of Dinghai sea area. Based on physiological and biochemical tests together with molecular analysis, it was designated as Pseudoalteromonas sp. R3. To arrive at a better understanding and address the commercializa- tion of LAAO from this isolate, future works are planned to clone the gene coding for this Pseudoalteromonas sp. R3 LAAO and to study its structure, biological and phy- siological roles, relationship between function and struc- ture, and mechanism of transcription in vivo.
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Anticoagulant and cytotoxic assessment of L-amino acid oxidase from Indian cobra (Naja naja) venom

Anticoagulant and cytotoxic assessment of L-amino acid oxidase from Indian cobra (Naja naja) venom

lant and anti-bacterial properties, platelet aggregation, apoptosis and edema. These effects are attributed to the release of high amounts of H2O2, a known reactive ox- ygen species (ROS), during the reaction. According to Ande et al. (4), ROS is formed extracellularly and acts directly by altering cell membrane permeability, and is also involved in cell apoptosis. Anticoagulant proteins have a major contribution in the mechanism of blood coagulation. Thrombosis and hemostasis are the major targets of snake venom proteins and can provide po- tential for designing and developing new drugs to pre- vent/treat blood clotting disorder. Few in vitro studies have reported the possible involvement of snake ven- om proteins and their components, in inhibiting blood clot formation (5). Sakurai et al. reported that purified LAAO from A. h. blomhoffii venom possesses antico- agulant activity (6). Several venom proteins including PLA2, LAAO and proteases exhibit their enzymatic anticoagulant properties though different mechanisms. Snake venom proteins with molecular masses between 6 kDa and 35 kDa are said to prolong coagulation and inhibit blood coagulation (7). Cancer is one of the leading causes of death worldwide, and hence there is an urgent need for a better treatment for cancers. The search to cure cancer using natural resources has been in practice for long time as surgery, radiotherapy and chemotherapy do not provide adequate protection against cancer cells. Current cancer treatment methods simultaneously affect normal cells along with cancer- ous cells, causing more serious side effects. Calmette in 1993 first reported with the use of snake venom as a treatment for cancer in laboratory animals (8). Venoms from elapidae, viperidae and crotalide were found to have cytotoxic properties against B16F10 melanoma cell lines. The cytotoxicity was stronger in elapidae venom compared to viperidae and crotalide in causing cell aggregations (9). But this anticancer agent involves various side effects, that are toxic to normal cells and thus decreases its therapeutic indexes (10). This finding led the research for identifying other agents for cancer treatment from naturally available products. The aim of the present study was to further investigate the antico- agulant properties and the mechanism of cytotoxicity of the purified enzyme – L-amino acid oxidase (Nn- LAAO) from the Indian cobra (Naja naja) venom, be- longing to the western part of the Indian subcontinent, against two human cancer cell lines, including Human colorectal carcinoma (HCT 116) and breast cancer cell lines (MDA MB-231).
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Physiological genetics of transport systems for amino acids in Neurospora crassa

Physiological genetics of transport systems for amino acids in Neurospora crassa

Page II 82 "" e sults c hlp-2 and other hlp mutants a b Introduction 85 86 esults Physiological defects of the hlp-1 Chapter IV and hlo-2 mutants Histidine uptake via the aromatic amino [r]

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GENETICS OF ARGININE BIOSYNTHESIS IN NEUROSPORA CRASSA

GENETICS OF ARGININE BIOSYNTHESIS IN NEUROSPORA CRASSA

The arginine auxotrophic class, moreover, was uniformly unable to use ornithine as an arginine source (Table 2 ). This is consistent with the ornithine transcarba- mylase deficiency ch[r]

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MUTATION AT THE am LOCUS OF NEUROSPORA CRASSA

MUTATION AT THE am LOCUS OF NEUROSPORA CRASSA

For purposes of standardization, we arbitrarily chose 25 % protection as the cut-off point between mutants assigned as CRM-negative (25% or less protection) and CRM-p[r]

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FIXED GENETIC INSTABILITY IN NEUROSPORA CRASSA

FIXED GENETIC INSTABILITY IN NEUROSPORA CRASSA

Were this the case, the two unstable alleles would rarely mutate to a stable state (this would require a transversion) but rather, each would mutate spontaneously by tra[r]

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A NEW INCOMPATIBILITY LOCUS IN NEUROSPORA CRASSA

A NEW INCOMPATIBILITY LOCUS IN NEUROSPORA CRASSA

CDe, nt, A x cdE, nt, a, and CDe, inos, A x CDE, nt, a, but in the latter cross the heterocaryon genotypes of the wild-type pairs obtained were deduced from the types sh[r]

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MUTANTS OF NEUROSPORA CRASSA PERMEABLE TO HISTIDINOL

MUTANTS OF NEUROSPORA CRASSA PERMEABLE TO HISTIDINOL

The observation that the growth of his-3 hlp-2 on histidine is strongly inhibited by neutral amino acids indicates that the mutation modifies the basic amino acid... The only.[r]

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MITOSIS IN VEGETATIVE NUCLEI OF NEUROSPORA CRASSA

MITOSIS IN VEGETATIVE NUCLEI OF NEUROSPORA CRASSA

While seven also appeared in most of the cells observed in the present study, a very small eighth chromosome has been noted in several metaphase figures (Figure 32).. This eighth [r]

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