[PDF] Top 20 Studies on the DNA of bacteriophage T7
Has 10000 "Studies on the DNA of bacteriophage T7" found on our website. Below are the top 20 most common "Studies on the DNA of bacteriophage T7".
Studies on the DNA of bacteriophage T7
... the relation between molecular weight and cr Yeandle 6 rederived to include the electrical effects expected from the polyanionic character of the DNA, and confirmed 1 that even if the DN[r] ... See full document
A Mutation in the Gene-encoding Bacteriophage T7 DNA Polymerase That Renders the Phage Temperature-sensitive
... of bacteriophage T7 encodes a DNA polymer- ase essential for phage ...detectable T7 DNA synthesis in vivo. DNA polymerase activity in these phage-infected cell extracts is ... See full document
A Complex of the Bacteriophage T7 Primase-Helicase and DNA Polymerase Directs Primer Utilization
... a DNA duplex, DNA primase periodically deposits short RNA primers at specific priming sequences on the lagging strand, triggering the syn- thesis of Okazaki fragments that are subsequently processed to form ... See full document
Amino Acid Changes in a Unique Sequence of Bacteriophage T7 DNA Polymerase Alter the Processivity of Nucleotide Polymerization
... domain. T7 DNA polymerase purified in the ab- sence of thioredoxin is subject to proteolytic attack at three clustered sites that lie within the COOH-terminal region of the protein, and this proteolysis is ... See full document
A unique loop in the DNA-binding crevice of bacteriophage T7 DNA polymerase influences primer utilization
... of bacteriophage T7 DNA poly- merase reveals the presence of a loop of 4 aa (residues 401– 404) within the DNA-binding groove; this loop is not present in other members of the DNA ... See full document
Rescue of Bacteriophage T7 DNA Polymerase of Low Processivity by Suppressor Mutations Affecting Gene 3 Endonuclease
... of T7 phage. The self-sufficient ge- nome organization of bacteriophage T7 and its robust lytic life cycle have made it an attractive model to investigate genetic adaptation under environmental ... See full document
An Interaction between DNA Polymerase and Helicase Is Essential for the High Processivity of the Bacteriophage T7 Replisome
... of bacteriophage T7 and variants of ...of bacteriophage T7 contains DNA polymerase (gp5) and its pro- cessivity factor, ...(trx), DNA primase-helicase (gp4), and ssDNA-binding ... See full document
Studies on T even bacteriophage DNA
... To map the deletion in T5stO on the T5 chromosome by electron microscopy, the intact strand of T5stO is hybridized with a mixture of two fragments as shown in Fig... Schematic representa[r] ... See full document
Intracellular organization of bacteriophage T7 DNA: analysis of parenteral bacteriophage T7 DNA-membrane and DNA-protein complexes.
... The dialyzed membrane complex was further purified by centrifugation in a second cesium chloride gradient under identical conditions described above.. At the end of the centrifugation, f[r] ... See full document
Multidimensional analysis of intracellular bacteriophage T7 DNA: effects of amber mutations in genes 3 and 19.
... The 32S to 100S linear DNA also contained TJ restriction endonuclease fragments, predominantly the TJL-R fragment expected of a concatemer that consists of monomeric DNAs joined left end[r] ... See full document
Characterization of the defects in bacteriophage T7 DNA synthesis during growth in the Escherichia coli mutant tsnB.
... Our observation that the defects which occur in phage DNA synthesis during infection of tsnB cells closely resemble those which occur during infection of a wild-type cell with a T7 gene [r] ... See full document
Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA
... The positions of promoter and terminator sites for the T7-specific RNA polymerase were estimated from the molecular weights of transcripts from exonucleolytically shortened templates 7; [r] ... See full document
Revised transcription map of the late region of bacteriophage T7 DNA.
... Through the use of an agarose-formaldehyde gel system that permits the resolution of high-molecular-weight RNAs, transcription of template DNA that had been cut with various restriction [r] ... See full document
Evidence for bacteriophage T7 tail extension during DNA injection
... The needle-like extension associated with the capsid indi- cated by arrow 5 of Figure 3 (magnified in the inset) is seen more clearly to penetrate the attached vesicle and has a length of 41 nm, but also appears to have ... See full document
Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.
... When extracts were incubated at 30°C and the intracellular DNA was subsequently extracted and digested with BstNI plus BglI, a novel anomalous DNA fragment 49% of the length of mature DN[r] ... See full document
Folded, concatenated genomes as replication intermediates of bacteriophage T7 DNA.
... More recently, the nonionic detergent Brij-58 was shown to produce a similarly fast-sedimenting structure, which in electron micrographs appeared to consist of up to 30 phage equivalents[r] ... See full document
Electron microscopic analysis of partially replicated bacteriophage T7 DNA.
... 45[Suppl.]:51-74, 1955 as an explanation for the distribution of marker rescue frequencies during cross-reactivation; i.e., replication proceeds in a bidirectional manner from an origin [r] ... See full document
In vitro recombination of bacteriophage T7 DNA damaged by UV radiation.
... DNA from T7%8 phage was irradiated and incubated with extracts from T73- or T73,6-infected strain W3110 under conditions that would permit both replication and recombination.. Controls n[r] ... See full document
Association of Replicating Bacteriophage T7 DNA with Bacterial Membranes
... The major portion of the newly synthesized T7 DNA cosedimented Control Pronase Trypsin DNase RNase Phospholipase A Percent of control exhibiting tast sedimentation rate Membranes DNA 10 [r] ... See full document
Role of gene 2 in bacteriophage T7 DNA synthesis.
... It was observed that after infection with T7 wild type a major portion of the newly synthesized DNA sedimented in both alkaline and neutral sucrose gradients 1.3 to 1.6 times as fast as [r] ... See full document