[PDF] Top 20 Subcellular Localization of Hepatitis C Virus Structural Proteins in a Cell Culture System That Efficiently Replicates the Virus

... Antibodies. Rat MAb 3/11 (19) was produced in vitro by using a MiniPerm apparatus (Heraeus) as recommended by the manufacturer. Mouse anti-E2 MAb AP33 has been previously described (7). Human anti-E1 MAb 1C4 (Inno- ... See full document

... analyzed on agarose gels (Bredenbeek et al., submitted). No viral RNA synthesis was detected in cells transfected with chimeras 1.0 and 1.3 (Fig. 2A, lanes 2 and 5). In contrast, chimeras 1.1 and 1.2 and the YF-17D ... See full document

... recombinant proteins derived from infectious agents has begun to replace the more traditional approach to vaccine design, with great success ...robust culture system for the virus and (ii) ... See full document

... the cell adhesion molecules E-Cadherin and β-Catenin were membrane localized both in 2-D and 3-D Huh7 cultures; however, there was a profound decrease in the accumulation of nuclear β-Catenin-containing com- ... See full document

... Hepatitis C virus (HCV) is the most common etiologic agent of posttransfusion and sporadic non-A, non-B hepatitis (6, ...mature structural and nonstructural proteins (39). The ... See full document

... the structural proteins, and formation of native glyco- protein complexes, we examined whether cells carrying the sfl genome produced and released HCV ...cleared cell culture supernatant to ... See full document

... worldwide, Hepatitis C virus (HCV) infection and associated liver diseases (hepatic cirrhosis, fibrosis and hepatocellular carcinoma) are significant health concerns in developing world and even in ... See full document

... non- structural proteins with paracrystalline structures reminiscent of the MT aggregates detected in HCV-infected chimpanzee hepatocytes or near sites of viral RNA synthesis in Kunjin virus-infected ... See full document

... produce virus particles from constructs encoding GFP- tagged p7 were not successful (data not ...infectious virus in a trans-complementation setup but also in the full-length Jc1 infectious ...rescue ... See full document

... the C-terminal transmembrane domain of the p7 used in this work (HCV H strain), indicating that this domain has the characteristic structural features of a signal peptide ...the proteins used to ... See full document

... lication by determining the G418 transduction efficiency. Since this approach did not allow the direct determination of repli- cation kinetics, transient-replication assays in nonselected cells were performed. For this ... See full document

... The cell-cell fusion assay described here provides a simple, quantitative, and versatile tool to study HCV-mediated ...this system come from its several ...chronic hepatitis, it caused ... See full document

... E2 proteins in ...envelope proteins in ...was efficiently recovered in coimmunopre- cipitates with anti-human lactoferrin antibody from the cell lysates as well as from the cell ... See full document

... per cell (mean fluorescence intensity ...and replicates to high titers, suggesting that it may be a good candidate for establishing a complete replica- tion cycle in cell ...their C-terminal ... See full document

... or proteins, or variations in the level of an antiviral state induced by replicating HCV ...NS proteins and the PV IRES required for translation of the reporter gene ... See full document

... Subcellular localization of the NS2 and NS3 proteins of hepatitis C virus was ...analyze subcellular localization of NS3, NS3 D C1459 (aa 1027 to 1459), full-size ... See full document

... The possible mechanisms can be studied by using simian virus 40 SV40-infected cells, since viral structural polypeptides are transported into the cell nucleus for assembly into virus By [r] ... See full document

... mosaic virus (NCMV), Rice yellow stunt virus (RYSV), and Sonchus yellow net virus (SYNV) (4, 5, 6, 9, 11, 17, 19, 21, 28, 29, 36, 39, 41, 43, 44, ...planta subcellular localization ... See full document

... infectious virus, our data are not entirely consistent with a role for p7 solely as an ion channel involved in ...infectious virus has recently been shown to be preceded by the accumulation of intracellular ... See full document

... The distribution of the agnoprotein was determined by immunoprecipitation of the same subcellular fractions with either the rabbit antiserum directed against the purified agnoprotein or [r] ... See full document