[PDF] Top 20 Synthetic Biology Approaches for Combinatorial Biosynthesis of Polyketide Natural Products.

... of combinatorial biosynthesis platform for polyketide ...chemical biology tools for probing the promiscuity of various ...for polyketide biosynthesis that were not accessible ... See full document

... Polyketide synthases (PKSs) are responsible for the production of diverse and complex natural products that often contain clinically relevant properties. Rationally engineering PKSs to produce higher ... See full document

... full-length products were examined, there were the expected masses for non-natural incorporation (4b-i), but there were also the masses of the unreduced 10-dML products ...pentyl products were ... See full document

... of synthetic biology entails the engineering of different biosynthetic enzymes, with a particular emphasis on the major players in NP biosynthesis, which are the multidomain PKSs and non-ribosomal ... See full document

... Prelactones are examples of δ-lactones isolated from bafilomycin producing microorganisms and other polyketide macrolide producing organisms. The structures of these compounds have been assigned on the basis of ... See full document

... chain release has also been proposed to occur in several fungal PKS systems, although direct biochemical evidence for this is currently lacking. 27–31 In contrast, relatively few bacterial modular PKSs have been ... See full document

... the use of synthetic herbicides, the use of natural products such as essential oils, plant extracts,.. 12.[r] ... See full document

... complex natural product biosynthesis, whose metabolic products were ...III polyketide synthase (PKS) enzymology and atypical non- ribosomal peptide synthetase (NRPS) biochemistry in germicidin ... See full document

... the biosynthesis of bioactive compounds in an “unnatural host” can prove challenging: hurdles of transcription and translation in the foreign host must first be overcome, enzymes must be correctly folded and may ... See full document

... engineering have become indispensable and commonplace in the past decade, and most researchers working on the biological side of chemistry use them extensively. The days of painstakingly purifying an enzyme from ... See full document

... adapted to their presence arises. Interestingly, there is variation in symbiont and bryostatin status within the genus Bugula, with some species possessing a symbiont that produces bryostatins, some species possessing a ... See full document

... 6-MSA biosynthesis was the first fungal PKS gene to be cloned and is also one of the best characterized fungal PKSs ...for biosynthesis of myco- toxin citrinin have been reported, in which, a pksCT plays an ... See full document

... The crude residue was purified by flash column chromatography 5:1 hexane / ethyl acetate to give the desired product 0.19 g, 0.70 mmol, 35% as a yellow oil... Chapter 7: Experimental.[r] ... See full document

... 90 At the outset of our studies to build the C-ring, we attempted a variety of inter- and intramolecular annulation strategies but were unable to affect formation of the daunting C-ring. Having few remaining options, we ... See full document

... of polyketide synthase genes [37] in a 30 kb cluster (Figure 3) including PKS12, AurR1, aurJ, aurF, gip1 and gip2 [36,38] within at least 11 reading frames ... See full document

... Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin Bl biosynthesis.. Molecular and General Genetics.[r] ... See full document

... Comparison of the published Streptomyces genome sequences reinforces the idea that Streptomyces species are highly adaptable enabling them to cope with a variable and complex environment. The S. coelicolor genome ... See full document

... This rise in antibiotic resistance has been contrasted by a dramatic shift in the investment of big pharmaceutical companies away from antimicrobial research. Indeed, during the 1990s, many companies scaled back ... See full document

... valuable products, hosts, often plants, have been engineered by optimizing regulatory factors, increasing the expression of rate-limiting enzymes 11 ...high-value products from natural sources is ... See full document

... protein–ligand crystal structures for parasitosis, these five targets were not related to the above parasitosis in either humans or other animals. However, the findings suggested the potential of M. cordata to treat ... See full document