[PDF] Top 20 The N Terminus of Andes Virus L Protein Suppresses mRNA and Protein Expression in Mammalian Cells
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The N Terminus of Andes Virus L Protein Suppresses mRNA and Protein Expression in Mammalian Cells
... that expression of recombinant ANDV L protein in mammalian cells is severely impaired and that this phenotype is determined by the 534 N-terminal amino acid ...low ... See full document
11
Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
... in mammalian cells and in HeLa cell extract but not in ...to mRNA templates to make them trans- lationally ...293 cells, HeLa S10 extract, and postribosomal HeLa S100 ... See full document
6
A Chimeric Protein Containing the N Terminus of the Adeno-Associated Virus Rep Protein Recognizes Its Target Site in an In Vivo Assay
... Rep protein, the results are difficult to interpret in light of the interdependence of DNA recognition and other Rep ...Rep N terminus to a strong transcriptional activation ...tured mammalian ... See full document
11
Characterization of the RNA Silencing Suppression Activity of the Ebola Virus VP35 Protein in Plants and Mammalian Cells
... GFP mRNA within the areas of ...target mRNA, leading to reduced steady-state ...GFP mRNA (barely detectable in the three-component system) was present in plants that received the negative control ... See full document
12
Evaluation of Novel Human Immunodeficiency Virus Type 1 Gag DNA Vaccines for Protein Expression in Mammalian Cells and Induction of Immune Responses
... to mRNA instability ...Gag protein can result in efficient expression of Gag in a Rev- and RRE-independent fashion (29, ...DNA expression vectors and compared the influence of the constitutive ... See full document
8
An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
... nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was ...The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with ... See full document
10
Role of the C Terminus of Lassa Virus L Protein in Viral mRNA Synthesis
... full-length L protein in biochemical assays have not met with success so far (unpublished ...C terminus in cap binding, it underlines that var- ious hypotheses have to be taken into account to ... See full document
5
Expression of processed core protein of hepatitis C virus in mammalian cells.
... Detection of antibody against expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for post- transfusion hepatitis. Hepatitis C virus infe[r] ... See full document
7
Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells.
... The putative envelope protein of hepatitis C virus (HCV) was expressed in insect cells by using a baculovirus expression vector and in monkey COS cells under the control of exogenous pro[r] ... See full document
7
Creation and expression of myristylated forms of Rous sarcoma virus gag protein in mammalian cells.
... While it is clear that the viral protease is essential for processing the various forms of Pr76 to mature products in mammalian cells, it nevertheless appears that another pro-. tease ac[r] ... See full document
13
Selective inhibition of protein synthesis in virus-infected mammalian cells.
... Clearly, however, gougerotin, blasticidin S, and edeine cause a specific inhibition of protein synthesis in EMC-infected cells, as measured by the incorporation of [3,4-3H]proline into p[r] ... See full document
9
The role of de novo protein synthesis and SIRT1 in ER stress-induced Atf4 and Chop mRNA expression in mammalian cells
... Atf4 mRNA expression also occurs independently of de novo protein ...Chop mRNA expression is dependent upon PERK activation and eIF2 α phosphorylation, thus supporting and extending ... See full document
29
Recombinant IgM expression in mammalian cells: A target protein challenging biotechnological production
... a Ref. [36,37]; b Ref. [38]; c Ref. [40]. Despite this fast-paced biological field the biotechno- logical aspect of producing these IgM antibodies is trail- ing behind. Evaluation of the full potential of IgM as ... See full document
6
A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.
... fluorescent protein (gfp) cDNA that is adapted for high-level expression in mammalian cells, especially those of human ...for expression in mammalian ...adeno-associated ... See full document
15
Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells
... VP protein levels did not result in intact vector particles for any of the serotypes studied here, implying that VP proteins do not spontaneously self-assemble and form infectious particles in cells once a ... See full document
30
The Gammaherpesvirus Chemokine Binding Protein Binds to the N Terminus of CXCL8
... the N terminus of CXCL8 that binds to vCKBP-3, with the greatest contribution coming from the ...the N-loop of CC chemokines for binding, since removal of the N terminus of CCL2 ... See full document
5
La Crosse virus infection of mammalian cells induces mRNA instability.
... La Crosse virus infection of BHK cells leads to a dramatic shutoff of not only host protein synthesis but also viral protein synthesis later in infection.. This shutoff can be accounted [r] ... See full document
6
Andes Virus Nucleocapsid Protein Interrupts Protein Kinase R Dimerization To Counteract Host Interference in Viral Protein Synthesis
... hantavirus-infected cells do not undergo translational shutdown and fail to establish an antiviral state during the course of viral ...that Andes virus infection induced PKR ...that Andes ... See full document
12
Pseudotyping exosomes for enhanced protein delivery in mammalian cells.
... target cells. Here, we demonstrate how a vesicular stomatitis virus glycopro- tein (VSVG) can both load protein cargo onto exosomes and increase their delivery ability via a pseudotyping ... See full document
19
Pseudotyping exosomes for enhanced protein delivery in mammalian cells
... target cells. Here, we demonstrate how a vesicular stomatitis virus glycopro- tein (VSVG) can both load protein cargo onto exosomes and increase their delivery ability via a pseudotyping ... See full document
18
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