[PDF] Top 20 Ty3 Integrase Is Required for Initiation of Reverse Transcription
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Ty3 Integrase Is Required for Initiation of Reverse Transcription
... IN-mutant Ty3 and a transposition target ...the Ty3 and target plasmids as a control (see Materials and ...wild-type Ty3 showed normal levels of CA ...mutant Ty3, CA (data not shown) and IN ... See full document
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Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription.
... in reverse transcription and indicate that CyPA is required for a very early event in the infectious cycle, prior to the initiation of reverse ... See full document
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Overlapping retrovirus U5 sequence elements are required for efficient integration and initiation of reverse transcription.
... Mutations that disrupt the U5-IR stem cause a replication defect associated with both a decrease in synthesis of viral DNA in infected cells and a decrease in initiation of reverse trans[r] ... See full document
9
Interaction between retroviral U5 RNA and the T psi C loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription.
... To examine whether the S6 mutation could be complemented by base changes in the primer, we reconstituted a reverse transcription complex in vitro by utilizing as primers synthetic oligor[r] ... See full document
9
Prototype Foamy Virus Protease Activity Is Essential for Intraparticle Reverse Transcription Initiation but Not Absolutely Required for Uncoating upon Host Cell Entry
... R etrovirus-encoded enzymatic activities, especially those with protease (PR), reverse transcriptase (RT), and integrase (IN) functions, play essential roles in the viral replication cycle. For example, ... See full document
14
A retroviral RNA secondary structure required for efficient initiation of reverse transcription.
... Second, U5 deletion and leader substitution mutations which would disrupt the U5-leader stem structure in ATVA6, ATVA15, ATVA21, and ATVAL resulted in a decrease in the efficiency of vir[r] ... See full document
9
A specific orientation of RNA secondary structures is required for initiation of reverse transcription.
... As well as the interaction of the PBS with the tRNA primer, there are a series of secondary structures, U5-IR stem, U5-leader stem, and U5 RNA-T4iC interaction region, all required for e[r] ... See full document
8
Functional and Structural Dynamics of Hepadnavirus Reverse Transcriptase during Protein-Primed Initiation of Reverse Transcription: Effects of Metal Ions
... (either initiation or polymerization) (in the presence of Mg 2⫹ ), despite being a potent inhibitor of HBV DNA elon- gation (23, ...the initiation reaction by any RT protein tested with either Mn 2⫹ or Mg ... See full document
12
Distinct Requirement for Two Stages of Protein-Primed Initiation of Reverse Transcription in Hepadnaviruses
... interactions required for pgRNA packaging may impose special constraints for TP- RT-ε RNA interactions that permit the initiation ...protein-primed initiation of reverse ... See full document
9
Human Immunodeficiency Virus Type 1 Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex
... the initiation and elongation complexes are ...is required either prior to or at the initiation stage of reverse ...in reverse transcription in ...the reverse transcrip- ... See full document
10
Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription.
... is required to obtain detectable reaction products, and inhibition of reverse transcription by excess primer tRNA, using 50 ng of RT per reaction, is not a ... See full document
7
Ty3 integrase mutants defective in reverse transcription or 3'-end processing of extrachromosomal Ty3 DNA.
... disrupted reverse transcription, whether measured in an in vitro assay or by monitoring appearance of the ...5.4-kbp Ty3 VLP DNA in ...against Ty3 IN and was found only in cells expressing ... See full document
11
Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription
... in reverse transcription was also seen in endogenous reverse transcription ...is required at early stages of reverse transcription, al- though its exact role in this ... See full document
10
Molecular Mechanisms by Which Human Immunodeficiency Virus Type 1 Integrase Stimulates the Early Steps of Reverse Transcription
... vitro reverse transcription assays, and different results have been reported (18, 41, ...of reverse transcription that is monitored or the manner by which the in vitro reverse ... See full document
10
Role of Integrase in Reverse Transcription of the Saccharomyces cerevisiae Retrotransposon Ty1
... the initiation of reverse transcription has been demon- strated for ...of reverse transcription (18, 39, 42), while other muta- tions increased virus fitness by augmenting the ... See full document
9
Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication
... for reverse transcription during the viral life cycle and that per- turbing this interaction will lead to a defect specifically at the re- verse transcription ...the reverse ... See full document
12
Contribution of the C terminal tri lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import
... be required for reverse transcription, integration or undefined post-nuclear entry steps ...is required for viral DNA nuclear import ... See full document
15
Mutations in Nonconserved Domains of Ty3 Integrase Affect Multiple Stages of the Ty3 Life Cycle
... of reverse-transcribed Ty3 DNA observed associated with ...that Ty3 RT is a heterodimer, composed of RT-IN together with RT, such as the RT heterodimers of avian ...in Ty3 mutants truncated in ... See full document
13
A Chimeric Ty3/Moloney Murine Leukemia Virus Integrase Protein Is Active In Vivo
... element Ty3 is more closely related to the Drosophila melanogaster gypsy-like retroviruses and to animal retroviruses than to the other yeast retrotransposons ...(29). Ty3 is composed of a ...of Ty3 ... See full document
11
Cooperation between Reverse Transcriptase and Integrase during Reverse Transcription and Formation of the Preintegrative Complex of Ty1
... enzymes, integrase (IN) and reverse trans- criptase (RT), play a central role in the replication and trans- position of retroviral and retrotransposon ...retrotransposon Ty3 RT is an ␣ /  het- ... See full document
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