[PDF] Top 20 Unbiased Parallel Detection of Viral Pathogens in Clinical Samples by Use of a Metagenomic Approach
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Unbiased Parallel Detection of Viral Pathogens in Clinical Samples by Use of a Metagenomic Approach
... Previous metagenomic studies on viromes have employed a combination of filtration and density-dependent centrifugation to enrich for the majority of (but not all) viruses ...this approach may introduce a ... See full document
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Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
... for metagenomic sample-to-detection has now been reduced to <6 hr with nanopore sequencing, many challenges remain for rou- tine implementation of this technology in clinical and public health ... See full document
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Role of Clinicogenomics in Infectious Disease Diagnostics and Public Health Microbiology
... the detection of microbial ...for clinical infectious disease ...the use of specific PCR primers, it is also strongly biased compared to random shotgun ...of pathogens that exhibit little or ... See full document
8
Metagenomic Approach for Identification of the Pathogens Associated with Diarrhea in Stool Specimens
... Pathogen detection by the diagnostic laboratory and research labo- ...the clinical diagnostic laboratory, a glutamate dehydrogenase enzyme immunoassay (EIA) (Wampole ...Positive samples were con- ... See full document
8
Evaluation of Targeted Next Generation Sequencing for Detection of Bovine Pathogens in Clinical Samples
... the pathogens that may be present in a sample, enabling universal unbiased pathogen ...to metagenomic sequencing is targeted ...massive parallel sequencing. Compared to metagenomic ... See full document
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Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture
... ABSTRACT Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic testing that allows simultaneous detection of a broad range of vi- ruses, detailed taxonomic ... See full document
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Diagnostic metagenomics : potential applications to bacterial, viral and parasitic infections
... the detection and characterization of bacteria under the microscope using the staining technique developed by Hans Christian Gram and their propagation as colonies on solid growth media, as pioneered by Robert ... See full document
8
Human Picobirnaviruses Identified by Molecular Screening of Diarrhea Samples
... of clinical samples are nowadays per- formed largely using viral-species-specific ...preventing detection of virus ...of clinical diarrhea samples that tested negative for known ... See full document
8
Unbiased Metagenomic Sequencing for Pediatric Meningitis in Bangladesh Reveals Neuroinvasive Chikungunya Virus Outbreak and Other Unrealized Pathogens
... and viral pathogens are known to cause meningitis, but most microbiology laboratories in resource-poor settings test primarily for bacterial pathogens, namely, Neisseria menin- gitidis, Streptococcus ... See full document
12
Ochratoxin A Detection in Rice Samples in Mazandaran Province
... milk. OTA has already been proven to be instrumental in producing SOS, SCE (sister chromatid exchange), DNA repair outside living organisms and DNA strand separation in the liver and kidney of rats [3, 4]. OTA ... See full document
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Evaluation of a Novel Multiplex High Definition PCR Assay for Detection of Tick Borne Pathogens in Whole Blood Specimens
... distinct pathogens or pathogen groups associated with tick-borne ...HDPCR approach has previously been applied to successfully identify nine viral agents in simulated naso- pharyngeal specimens ... See full document
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Evaluation of the Vibrant DNA microarray for the high-throughput multiplex detection of enteric pathogens in clinical samples
... in detection of infectious ...of pathogens in a single ...the detection and identification of up to 20 pathogens in 1–5 h turnaround ...major pathogens [13]. The Verigene Table 6 ... See full document
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DNA Microarray Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients
... noncultured clinical samples containing only small amounts of fungal ...cultured clinical iso- lates. The detection of the fungal DNA was carried out using isolates and additionally spiked ... See full document
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Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples
... multiplexed viral pathogen reagent (11/242-001, National Institute for Biological Standards and Control, South Mimms, UK) containing 25 different human viruses from different virus families [52] (Table ...same ... See full document
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Simultaneous Detection of Pathogens in Clinical Samples from Patients with Community Acquired Pneumonia by Real Time PCR with Pathogen Specific Molecular Beacon Probes
... The target genes for the six pathogens were selected for the following reasons. The lytA gene encoding autolysin enzyme as well as the pneumolysin gene are specific to S. pneumoniae (26, 29); therefore, the lytA ... See full document
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Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples
... Bioinformatic analysis. Nanopore reads were base called using Guppy (Oxford Nanopore Technol- ogies, Oxford, UK). Output base called fastq files were demultiplexed using Porechop v0.2.3 (https:// ... See full document
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Investigation of Electrochemical Behaviour of Chromium(VI)-Dithiocarbamate Complexes: Detection of Chromium(VI) in Real Samples
... Chennai, India. All the samples were prior concentrated to 100 ml for easy detection of analyte. The obtained results are tabulated in Tables 3-4. 10 g of Triticum aestivum (Wheat) and 5 g of Spinacia ... See full document
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Multicenter Clinical Validation of the Molecular BD Max Enteric Viral Panel for Detection of Enteric Pathogens
... among samples positive for sapovirus and/or ...prospective samples, these differences were not statistically significant, with the exception of astrovirus, for which a difference in NPA between the ... See full document
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Improved Detection of Five Major Gastrointestinal Pathogens by Use of a Molecular Screening Approach
... for detection of the five enteric pathogens were previously described (4, 21, 34, 43, 45), and for some we previously performed extensive clinical validations (32, 33, ...of detection (LOD), ... See full document
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The AI-Discovered Aetiology of COVID-19 and Rationale of the Irinotecan+Etoposide Combination Therapy for Critically Ill COVID-19 Patients
... Demiurge Technologies AG is a Swiss research-based AI-biopharmaceutical company that transforms ultra-large- scale publicly available life science data into ultra-fine-detail precise disease models in areas with unmet ... See full document
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