[PDF] Top 20 Using RNA as molecular code for programming cellular function

... As seen earlier, sRNA binding to the mRNA can abolish translation initiation or elongation, thereby allowing direct control over protein expression in the cell. Recent work has expanded this approach to implement a ... See full document

... using siRNA showed that there is a significant reduction but not complete loss of Oct4 mRNA and protein level (Fig. S9D). The expression of key stem cell markers (i.e. Gfra1 and Id4) remained unchanged, while 590 ... See full document

... with cellular context and between species, ...in cellular mRNAs under different ...poly(A)+ RNA across a panel of murine tissues with highest levels in liver, kidney and ...poly(A)+ RNA ... See full document

... an RNA-dependent RNA polymerase (RdRp) and is therefore essential for the production of all viral proteins, including itself: the L protein participates in transcription of viral mRNAs by effecting its ... See full document

... molecules per microgram of RNA in the liver of uremic animals. This was associated with increased ribonuclease activity in uremic cytosol. The diminished albumin synthesis by membrane-bound polysomes of uremic rat ... See full document

... by RNA sequencing (RNA-Seq), there was significant upregulation of BRCA1-mediated signaling and G1/S cell cycle checkpoint ...the RNA-Seq data that represents the fully annotated catalogue of the ... See full document

... computer programming, a code smell is an indicator in the source code of a program that can display a deeper ...problem. Code fragrances are not generally bugs, they are technically incorrect ... See full document

... step in this pathway, the transcription factor IFN reg- ulatory factor 3 (IRF3) becomes phosphorylated on serine residues by a still-unidentified kinase. Activat- ed IRF3 then stimulates transcription of IFN- α 4 and ... See full document

... generated code mustn't be modified, conjointly strategy is defined like “when” and “where” to optimize for playing ...intermediate code, others area unit applied to final code generation and even a ... See full document

... of using pair programming from different perspectives and criteria, such as gender, course, specialization, and student ...pair programming than that those in other ... See full document

... nucleic acids [70]); and (b) the protein-encoding nucleic acid sequences themselves (which are the templates for protein syntheses but are not defined as chaperons). The idea that the nucleotide sequence itself could ... See full document

... In the present study, we began our investigation by searching the assembled transcriptome for ortholo- gous genes to known alkaloid transporter genes in the tobacco. Nt-JAT1 transports nicotine and other alkaloids in a ... See full document

... fractional programming Model (MOQFPM) are studied due to the fact that various real life conditions such as purchase/cost exist where several inter-related objectives are to be satisfied which are generally ... See full document

... dual function co-regulator of transcription factors Steroid receptor RNA Activator (SRA) was the first mammalian RNA to be discovered with dual roles, protein coding and non- coding, in myogenic ... See full document

... Software engineering has undergone a paradigm shift as the size of the software systems deployed increased dramatically and businesses began to rely increasingly on computers and information systems (Ramasubbu, 2001). A ... See full document

... FISH would be deemed “noisy”, or far from Poisson noise levels. We calculated CV 2 for mRNA counts as well as mRNA concentration (counts/volume) for many genes in our fibroblast cell line. Both of these measures assign ... See full document

... lists using now well-established classification systems such as GO can help put the work into an intellectual framework that provides the opportunity for hypothesis generation and mechanistic ... See full document

... infection. Using cDNA microarrays hybridized to cDNAs made from thiolated mRNAs, a small set of host transcripts was identified and their expression verified by quantitative PCR and Northern and Western blot ... See full document

... maintain RNA replication over the longer time period might result from defects in the quantity or functional quality of the RNAs pro- duced by ...after RNA replication had ...extracted RNA in fresh ... See full document

... upregulated RNA synthesis and their inability to establish persistent infec- tions (17–19, ...the molecular basis for the unique temporal regulation of the NS2 autopro- tease in noncp BVDV-infected ...a ... See full document