Top PDF Vaccinia Virus L1 Protein Is Required for Cell Entry and Membrane Fusion
Vaccinia Virus L1 Protein Is Required for Cell Entry and Membrane Fusion
... that L1 might be involved in cell entry was based on the following reasoning: (i) L1 is a target of neutral- izing antibody and is therefore exposed on the MV surface (40); (ii) the L1 ...
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The Vaccinia Virus A9L Gene Encodes a Membrane Protein Required for an Early Step in Virion Morphogenesis
... by protein A conjugated to 10-nm-diameter colloidal gold (Department of Cell Biology, Utrecht University School of Medicine, Utrecht, The Nether- ...Purified virus was adsorbed onto Formvar-coated ...
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Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins
... purified virus particles was previously shown by Western blotting using an epitope tag antibody (9) and by mass spectroscopy (4, ...recombinant virus vGFP-I2 was made by replacing the I2L ORF of the VACV ...
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Sequence-Divergent Chordopoxvirus Homologs of the O3 Protein Maintain Functional Interactions with Components of the Vaccinia Virus Entry-Fusion Complex
... A21 membrane protein, also part of the EFC, has a topology similar to that of ...chimeric protein failed to complement O3 function better than the vector control ...O3-inducible virus, as did ...
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Cell entry by measles virus: long hybrid receptors uncouple binding from membrane fusion.
... brane fusion in a cocultivation ...the membrane by four CD4 Ig-like domains (125 Å ...of fusion competence (protein 9). When the fusion efficiencies of molecules with one, two, or four ...
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N-Glycans on Nipah Virus Fusion Protein Protect against Neutralization but Reduce Membrane Fusion and Viral Entry
... viral entry and viral genome ...stomatitis virus (VSV) as described pre- viously ...viral entry, the Renilla luciferase reporter gene was cloned in place of the red fluorescence protein ...
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Cell-based analysis of Chikungunya virus E1 protein in membrane fusion
... Chikungunya virus (CHIKV) is an enveloped plus- stranded RNA virus classified into the genus Alphavirus of family ...capsid protein (C) and two envelope proteins (E2 and E1) ...viral membrane ...
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Association of Vaccinia Virus Fusion Regulatory Proteins with the Multicomponent Entry/Fusion Complex
... of vaccinia virus form a heterodimer (A56/K2) and have a fusion regulatory role as deletion or mutation of either causes infected cells to form large syncytia sponta- ...described entry ...
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Vaccinia Virus A56/K2 Fusion Regulatory Protein Interacts with the A16 and G9 Subunits of the Entry Fusion Complex
... of vaccinia virus (VACV) results in the spontaneous fusion of infected cells to form large multinucleated ...are required for interaction with the VACV entry fusion complex ...
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Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro
... Lymphoid cell lines (BJAB, human lymphoblastoid B cells, and B95a, an adherent subclone of Epstein-Barr virus-transformed marmorset B cells) were maintained in RPMI 1640 medium containing 10% fetal calf ...
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Vaccinia Virus J1R Protein: a Viral Membrane Protein That Is Essential for Virion Morphogenesis
... J1R protein is not required for viral protein synthesis in infected ...of cell lysates with an anti-core antibody recognized abundant p4a precursor accumulation and the ab- sence of mature 4a ...
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The Envelope Protein Encoded by the A33R Gene Is Required for Formation of Actin-Containing Microvilli and Efficient Cell-to-Cell Spread of Vaccinia Virus
... A33R protein, the small plaques of A33R mutants had comet ...A33R protein levels rather than a coincidental mutation elsewhere in the ...EEV membrane glycoprotein associated with comet formation, ...
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Vaccinia Mature Virus Fusion Regulator A26 Protein Binds to A16 and G9 Proteins of the Viral Entry Fusion Complex and Dissociates from Mature Virions at Low pH
... of vaccinia virus was pre- pared and purified by CsCl gradient centrifugation as previously de- scribed (18, ...vA28i virus, in which the A28L open reading frame (ORF) is under isopropyl-- D - ...
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Murine Coronavirus Requires Lipid Rafts for Virus Entry and Cell-Cell Fusion but Not for Virus Release
... MHV entry required ...target membrane directly or indirectly after virus binding to the ...to membrane flotation analysis, fol- lowed by immunoprecipitation with polyclonal anti-MHV an- ...
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The GP64 envelope fusion protein is an essential baculovirus protein required for cell-to-cell transmission of infection.
... integral membrane glycoprotein that has been studied in some detail (5, 8, 20, 25, 26, 28, 29, 35, 37, ...EFP protein (38), and these peplomers are acquired by the virion during ...low-pH-activated ...
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Disulfide Bonds and Membrane Topology of the Vaccinia Virus A17L Envelope Protein
... A17L protein, provided evidence for an intramolecular disul- fide bridge, and demonstrated that cysteine 178 is required for the former and cysteines 101 and 121 are required for the ...A17L ...
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The Lipid Raft-Associated Protein CD98 Is Required for Vaccinia Virus Endocytosis
... hybridoma cell line 4F2(C13) (anti-CD98) was purchased from the ATCC ...by protein A affinity ...fluorescent protein (GFP) monoclonal anti- body (JL8) was obtained from ...A4 protein purified ...
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Genetic Confirmation that the H5 Protein Is Required for Vaccinia Virus DNA Replication
... cytoplasmic membrane-delimited ...are required for efficient DNA synthe- sis; indirect evidence has suggested that the multifunctional H5 protein may also have a ...complementing cell line, ...
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The Envelope G3L Protein Is Essential for Entry of Vaccinia Virus into Host Cells
... Vaccinia virus, the prototype of the orthopoxvirus genus of the family Poxviridae, infects many cell lines and animals (9) and produces several forms of infectious particles, namely, intracellular ...
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Thiol/Disulfide Exchange Is Required for Membrane Fusion Directed by the Newcastle Disease Virus Fusion Protein
... F protein blocked cell-cell ...on cell-cell ...F protein cDNAs, as well as pTA, were treated with inhibitors or left ...F protein-expressing ...in cell extracts and ...
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