[PDF] Top 20 In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus.
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In vitro mRNA degradation system to study the virion host shutoff function of herpes simplex virus.
... Finally, although the wild-type vhs function induced accelerated in vitro turnover of both viral and cellular mRNAs, endogenous 28S rRNA was equally stable in extracts from mock-infected[r] ... See full document
11
The Effect of Herpes Simplex Virus Virion Host Shutoff Gene- a New Suicide Gene- on Tumor Cells
... Our study showed that the Vhs can effectively induce morphological changes and cell death in both tumor cell ...different mRNA in individual tumor cell lines needs a precise study to evaluate ... See full document
5
The Virion Host Shutoff Endonuclease (UL41) of Herpes Simplex Virus Interacts with the Cellular Cap-Binding Complex eIF4F
... In vitro mRNA degradation system ...vivo mRNA degradation ...in vitro transcription and translation, and equal amounts were assayed for eIF4F binding as described for ... See full document
5
Herpes Simplex Virus 2 Virion Host Shutoff Endoribonuclease Activity Is Required To Disrupt Stress Granule Formation
... with herpes simplex virus 2 (HSV-2) have a disruption in their ability to form SGs in response to treatment with arsenite, an inducer of oxidative stress ...defective virion host ... See full document
13
Control of mRNA stability by the virion host shutoff function of herpes simplex virus.
... The first demonstration that the vhsl mutation affects the half-lives of viral as well as host mRNAs was provided by the observation that, following reversal of a cycloheximide block pre[r] ... See full document
10
The Splicing History of an mRNA Affects Its Level of Translation and Sensitivity to Cleavage by the Virion Host Shutoff Endonuclease during Herpes Simplex Virus Infections
... Vhs degradation than unspliced mRNAs, this increased resistance should contribute to increased levels of spliced mRNAs, with increased synthesis of the proteins that they ...Vhs mRNA-degradative activity, ... See full document
13
The Virion Host Shutoff Protein (UL41) of Herpes Simplex Virus 1 Is an Endoribonuclease with a Substrate Specificity Similar to That of RNase A
... in vitro assay. Moreover, the pattern of degradation of the RNA substrates into fragments of discrete sizes unequivocally defined vhs as an ...in vitro activity of the purified GST-vhs protein were ... See full document
5
Herpes Simplex Virus 1 Counteracts Tetherin Restriction via Its Virion Host Shutoff Activity
... Tetherin mRNA and protein levels were analyzed at various times postinfection by RT-qPCR and Western blotting, respec- tively, in HeLa cells infected with HSV-1wt and ...tetherin mRNA, with a ...tetherin ... See full document
9
Role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis.
... by herpes simplex virus type 1 (HSV-1) requires the coordinated regulation of all three temporal classes of viral genes: immediate-early ( a ), early ( b ), and late ( g ) (20, ...the ... See full document
8
Herpes Simplex Virus Virion Host Shutoff (vhs) Activity Alters Periocular Disease in Mice
... Herpes simplex virus (HSV) causes the rapid shutoff of macromolecular synthesis in infected ...this shutoff is the product of the UL41 gene, known as the virion host ... See full document
7
Selective Ablation of Virion Host Shutoff Protein RNase Activity Attenuates Herpes Simplex Virus 2 in Mice
... The virion host shutoff (vhs) protein of herpes simplex virus (HSV) has endoribonuclease activity and rapidly reduces protein synthesis in infected cells through mRNA ... See full document
12
The Herpes Simplex Virus 1 Virion Host Shutoff Protein Enhances Translation of Viral Late mRNAs by Preventing mRNA Overload
... our study seem to be indepen- dent of eIF2 ␣ phosphorylation, as the level of eIF2 ␣ phosphor- ylation was comparable in ⌬Sma- and IEUS11-⌬Sma-infected ...striking host range phenotype, with translation of ... See full document
9
A Single Amino Acid Substitution in Herpes Simplex Virus Type 1 VP16 Inhibits Binding to the Virion Host Shutoff Protein and Is Incompatible with Virus Growth
... in vitro and in vivo studies strongly suggest that VP16 fulfills this role (28, ...VP16-null virus (8MA), which is defective in the production of infectious prog- eny because of the absence of newly ... See full document
11
Tristetraprolin Recruits the Herpes Simplex Virion Host Shutoff RNase to AU-Rich Elements in Stress Response mRNAs To Enable Their Cleavage
... key function of herpes simplex virus (HSV) gene products is to block host responses to ...by degradation of host macromol- ecules that are inimical to viral replication ... See full document
8
Herpes Simplex Virus 1 Abrogates the cGAS/STING-Mediated Cytosolic DNA-Sensing Pathway via Its Virion Host Shutoff Protein, UL41
... Human foreskin fibroblasts (HFFs) express many DNA sensors, including cGAS, and have been used to study their functions (15, 22). Immunostimulatory DNA (ISD) is a double-stranded DNA 60-mer oligonucleotide derived ... See full document
9
The Herpes Simplex Virus Host Shutoff RNase Degrades Cellular and Viral mRNAs Made before Infection but Not Viral mRNA Made after Infection
... synthesis system for real-time PCR (RT-PCR) (Life Technol- ogies), according to the supplier’s ...this study were reported previously ...StepOnePlus system (Applied Biosystems) and were analyzed with ... See full document
7
Picornavirus Internal Ribosome Entry Site Elements Target RNA Cleavage Events Induced by the Herpes Simplex Virus Virion Host Shutoff Protein
... for virus replication, vhs mu- tants display a ca. 10-fold reduction in virus yield in tissue culture (57, 62) and cause severe defects in the nervous system of the mouse (66), indicating that this ... See full document
10
mRNA Decay during Herpes Simplex Virus (HSV) Infections: Mutations That Affect Translation of an mRNA Influence the Sites at Which It Is Cleaved by the HSV Virion Host Shutoff (Vhs) Protein
... pBK2 mRNA to form a circle signifi- cantly reduces Vhs cleavage at the sites where it cuts capped linear ...classic study, Chen and Sarnow showed that circular mRNAs containing an EMCV IRES can be ... See full document
16
Mutational analysis of the herpes simplex virus virion host shutoff protein: evidence that vhs functions in the absence of other viral proteins.
... trigger shutoff, then it should strongly inhibit expression of an other- wise constitutively active b -galactosidase reporter gene in co- transfected mammalian cells (through a combination of direct and indirect ... See full document
9
mRNA Decay during Herpes Simplex Virus (HSV) Infections: Protein-Protein Interactions Involving the HSV Virion Host Shutoff Protein and Translation Factors eIF4H and eIF4A
... The observation that Vhs binds a cellular translation factor suggests obvious possibilities for how a sequence nonspecific endonuclease is targeted to mRNAs, as opposed to non-mR- NAs, and to preferred sites within mRNAs ... See full document
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