[PDF] Top 20 In vitro replication of mouse hepatitis virus strain A59.

... Before attempting an in vitro assay for coronavirus RNA synthesis, the kinetics of MHV-A59 polymerase activity in infected Sac- cells were determined.. To synchronize expression of the v[r] ... See full document

... of mouse hepatitis virus (MHV) are isogenic except for the spike gene, which encodes a glycoprotein expressed on the virion envelope that mediates many biological properties of MHV ...either ... See full document

... HCV replication after transfection of RNA into ...efficient replication of most HCV replicons in cell culture requires adaptive mutations in the viral genome ...HCV replication initially could only ... See full document

... rJHM strain used here is derived from the highly neurovirulent strain ...peritonitis virus (FIPV) spike protein ectodomain in place of the MHV-A59 and MHV-JHM ectodomains and were propagated ... See full document

... FIG. 1. Strategy to assemble an infectious cDNA of MHV-A59. (A) Esp3I cloning properties. In the traditional mode, an Esp3I site resides at the 3⬘ end of the MHV A subclone and upon cleavage results in a novel ... See full document

... of mouse hepatitis virus strain A59 (MHV-A59) encodes several putative functional domains, including three proteinase ...in vitro cleavage of p28, a polypeptide that is ... See full document

... MHV- A59- and MHV-2-infected mice were cut into 5- ␮ m longitudi- nal sections and stained with H&E and inflammatory cell ...MHV-2-infected mouse spinal cords. Serial cross sections (5 ␮ m thick) from ... See full document

... coronavirus mouse hepatitis virus strain A59 (MHV-A59) requires the release of the viral genome by fusion with the respective target membrane of the host ...that virus is ... See full document

... If certain characteristic spots in the RNase T1 oligonucleotide fingerprints of the virus-specific RNAs would be derived from such short 5'-terminal sequences, comparison of the fingerpr[r] ... See full document

... that virus variants with altered receptor specificity and host range can be selected during per- sistent infections in vitro in which the expression of the virus receptor is low, possibly with the ... See full document

... The putative defensive effect of Poly (I:C) described herein correlated with a similar “in vitro” activity of the TLR3-ligand on MHV-infected J774A.1 murine macrophages [22]. Thus, Mazaleuskaya et al. [22] found ... See full document

... The similarity of the IBV potential protease activities to those of the picornaviruses, the data from MHV-A59 genome in vitro translation, the presence of potential 3C protease-like clea[r] ... See full document

... coronavirus mouse hepatitis virus (MHV) strain A59 uses murine carcinoembryonic antigen-related cell adhesion mole- cule 1a as its receptor for cell entry, but S protein can also be ... See full document

... of virus evolution resulting from the interplay between the processes of RNA recombination and genome ...RNA replication (11), combined with the versatility of expression afforded by the use of translation- ... See full document

... inhibit hepatitis B virus (HBV) replication by eliminating pregenomic RNA containing viral capsids from the hepa- ...viral replication in these cells is efficiently controlled by IFN- ␣ / ␤ or ... See full document

... 3 0022-538X/85/030834-07$02.00/0 Copyright C 1985, American Society for Microbiology Three Intergenic Regions of Coronavirus Mouse Hepatitis Virus Strain A59 Genome RNA Contain a Common [r] ... See full document

... Little is known about DC function in vivo after infection with MHV. Dendritic cells are readily infected by MHV-A59 and form syncytia in vitro (45). The primary host cell receptor for MHV is CEACAM-1a, a ... See full document

... MHV A59, the I ORF is in the ⫹ 1 reading frame relative to the N ...in virus-infected cells and is present in purified virions (20, 55), but the function of the protein is not ...for replication of ... See full document

... mutant virus with reciprocal charge changes: R227D in the M proteins paired with our previously described D440R, D441R, or DD440-441RR muta- tions in the N protein ...of replication-competent genomic RNA, ... See full document

... any hepatitis in the livers of infected animals following intra- cerebral inoculation ...induce hepatitis. As we published previously, the level of replication of C12 in the liver following ... See full document