If you have this module enabled in HASP, some extra commands and features for analysis of multiple binary layers over Z become available.
9.2. 4D/6D Acquisition
The 4D/6D Acquisition module enables users to combine several acquisition types into one ND experiment. A multi-dimensional data set comes out from such an experiment.
9.3. API - Advanced Interpreter
This module dramatically extends the macro programing capabilities. It adds the following functionality to the main application:
User interaction functions
Contains functions, which enable interaction with user like: type value, text, combo boxes, list boxes and many other Windows standard controls with easy and intuitive code handling.
Support of importing functions from external DLLs
Functions from external DLLs can be imported at the start of the macro and used during the macro exe- cution.
Please see the electronic help for more details.
9.4. Calcium and FRET
Installing the [Calcium and FRET] module adds several tools to the system:
• A control window suited for capturing FRET images. • Automated FRET calibration algorithm.
• The [Create FRET Image] window for “passive” creation of FRET images from files. • Calcium ion concentration measurement ability.
Please see the electronic help for more details.
9.5. Database
If the database module was installed, the system can create and control MS Access databases (*.MDB). A database can help you to efficiently organize your image archives and manage additional information related to the images. The images themselves are stored on hard disk, only links to them and the additional data are stored in the database file.
After you install the Database module, the [Database] menu appears in the main tool bar. Please see the electronic help for more details.
9.6. AutoQuant Blind Deconvolution
The imperfection of a microscope optical path causes convolution of the original image signal, which makes the resulting image blurry. Deconvolution algorithms reduce the impact of convolution on the image. Even though “Blind” means that we do not have to know the convolution parameters accurately, some characteristics of the optical path (objective) shall be known in order for the deconvolution module to work properly. Please see the electronic help for more details.
9.7. AutoQuant Real-Time 2D Deconvolution
The imperfection of a microscope optical path causes convolution of the original image signal, which makes the resulting image blurry. Deconvolution algorithms reduce the impact of convolution on the image. The Real- Time Deconvolution module algorithm enables high speed processing and therefore is very suitable for use on Live signal from cameras. Real-time deconvolution can be applied to the Live image continuously or just at capture-time. It can be also used for processing saved images.
Please see the electronic help for more details.
9.8. LIM Deconvolution
(requires: [Local Option] )
The imperfection of a microscope optical path causes convolution of the original image signal, which makes the resulting image blurry. Deconvolution algorithms reduce the impact of convolution on the image. [Standard 2D/3D], [Blind 2D/3D] and [Fast (Wiener)] methods are available.
Please see the electronic help for more details.
9.9. Local Option
Local Option is a pseudo-additional module. When Local Option is installed (see Step 2), NIS-Elements AR will provide some advanced features which did not pass the quality assurance procedure yet. We recommend to wait until they are released officially.
9.10. Deconvolution (legacy)
The imperfection of a microscope optical path causes convolution of the original image signal, which makes the resulting image blurry. Deconvolution algorithms reduce the impact of convolution on the image. The al- gorithm used is “constrained iterative - maximum likelihood estimation algorithm” (the actual name of the al- gorithm is “Lucy-Richardson”).
Please see the electronic help for more details.
9.11. Peripheral Device
If a peripheral device is connected to NIS-Elements AR, some extra commands and features become available. This concerns typically motorized XY stages and Z drives, but also other devices. See [Connected Devices].
9.12. Extended Depth of Focus
The EDF module allows you to combine an existing Z-stack of images into one focused image by picking the focused regions from each frame and the pieces together.
9.13. Filters
This module is aimed at analysis of filters according to the ISO 16232 standard. Please see the electronic help for more details.
9.14. HDR
(requires: [Local Option] )
The High Dynamic Range module brings the functionality of creating HDR images within NIS-Elements AR. The following ways to create a HDR image are available:
Automatic capture
Call the [Applications > HDR > Capture Reflection Free Image] command and the HDR image will be captured using computer-estimated settings
Capture
When the [Applications > HDR > Capture HDR Image] command is called, you will be asked to adjust the HDR acquisition settings such as number of exposures, exposure time range, camera settings, etc.
Create from files
If a sequence of images which were captured using different exposure times is available, the [Applications > HDR > Create HDR Image] command can load the images and merge them into a HDR image.
Create from an ND file
Similarly to the situation when the HDR file is created from a sequence of images, it can be as well created from the frames of on nd2 file. Just run the [Applications > HDR > Create HDR from ND] command.
9.15. Metalo - Grain Size Analysis
After the module installation, a new item called [Metallography] appears in the [Applications] menu. Select the [Grain Size] command to display the grain size measurement layout.
Features of the Grain Size Analysis Module:
• Measurement of single-phase structures (e.g. Austenite, Aluminium).
• Measurement of two-phase structures (e.g. Ferrite-Austenite, Ferrite-Pearlite). • Planimetric, Linear, Circular, and Abrams measurement methods.
• Customization of the predefined measurement procedures.
• Support of ASTM and JIS standards (ASTM E112-96, ASTM E1382-97, JISG0551). • Easy data export.
Please see the electronic help for more details.
9.16. Metalo - Cast Iron Analysis
After the module installation, a new item called [Metallography] appears in the [Applications] menu. Select the [Cast Iron] command to display the cast iron measurement layout.
Features of the Cast Iron Analysis Module:
• Graphite classification according to ISO/ASTM/JIS standards (ISO 945, ASTM A247-06, JIS G5502). • Ferrite/pearlite ratio measurement.
• Customization of the predefined measurement procedures. • Easy data export.
Please see the electronic help for more details.
9.17. Time Measurement
The time measurement tool enables to record average pixel intensities within Regions Of Interest (ROIs) during a time interval. The measurement can be performed on Live camera signal or on a saved nd2 file. Before the measurement is started, regions of interest shall be defined and turned ON.
Please see the electronic help for more details.
9.18. Tracking
Motion characteristics of objects such as living cells can be measured automatically or manually using the [Tracking] control window.
Please see the electronic help for more details.
9.19. Advanced Tracking
(requires: [Local Option] )
If you have this module enabled in HASP, the [Tracking] module gets extended with several advanced features such as 3d tracking, etc.