Computer software used

All text and figures have been produced using Adobe Photoshop 6, Microsoft Office 97 suite and Microcal Origin 6. All traces were scanned into Adobe Photoshop 6 using a Hewlett Packard 4200C USB scanner (set to 300 DPI and 1:1 scale), processed and stored in the JPEG format. All I/V ramps were generated and analysed in Pclamp 8.1 (Axon instruments).

Table 2.11. Table of materials

Supplier

A c e ty lc h o lin e ch lo rid e (A C h )

A tro p in e m e th y l b ro m id e & a tro p in e s u lp h a te C a lc iu m h y d ro x id e

C a rb a m y lc h o lin e c h lo rid e (c a rb a c h o l) C o lla g e n a s e (T y p e lA ),

(-)-c y tis in e h y d ro c h lo rid e (C ys)

D ih y d ro -b e ta erythroidine h y d ro b ro m id e (D H p E ) 1 -1 -d im e th y -4 -p h e n y l-p ip e ra z in iu m io d id e (D M P P ) (+ )-E p ib a tid in e h y d ro c h lo rid e (E p i)

E th yl m -a m ln o b e n z o a te m e th a n e s u lp h o n a te s a lt (T ric a in e ) (-)-L o b e lin e h y d ro c h lo rid e (L o b )

M e c a m y la m in e h y d ro c h lo rid e M e th a n s u lp h o n ic acid M in e ra l oil (M -5 9 0 4 )

(-)-n ic o tin e h y d ro g e n ta rtra te (N ic)

P o ta s s iu m a c e ta te a n d p o ta s s iu m g lu c o n a te (+ )-tu b o c u ra rin e ch lo rid e

T riz m a h y d ro c h lo rid e (T ris; S ig m a U ltra )

S ig m a , U K.

T rim e th a p h a n c a m s y la te C a m b rid g e L a b o ra to rie s Ltd, W a lls e n d , T y n e a n d W e a r, U K

C a lc iu m ch lo rid e H iP e rS o lv (H P L C w a te r), M a g n e s iu m ch lo rid e N -2 -h y d ro x y e th y lp ip e ra z in e -N -2 -e th a n s u lp h o n ic a c id (H E P E S ) P o ta s s iu m ch lo rid e S o d iu m ch lo rid e , S o d iu m h y d ro g e n c a rb o n a te a n d S o d iu m h y d ro x id e S u c ro s e B D H L a b o ra to ry S u p p lie s, P o o le , U K (a n a ly tic a l g ra d e ) H e a t in a c tiv a te d h o rse s e ru m P e n ic illin /S tre p to m y c in (P e n /S tre p )

L ife T e c h n o lo g ie s Ltd, P a isle y, U K

0 .2 2 p m p o re sy rin g e filte rs (M ille x G P ) M illip o re , B e d fo rd , U S A.

F a lc o n s te rile m u ltiw e lls a n d d is h e s B e c k to n D ic k in s o n , N J, U S A

C la rk b o ro s ilic a te g la s s (G C 1 5 0 T F ) fo r p u llin g e le c tro d e s E le c tro d e h o ld e rs (Q S W -T 15 N )

s ilv e r e le c tro d e w ire an d s ilv e r c h lo rid e ba th g ro u n d p e lle ts

W a rn e r In s tru m e n t C o rp o ra tio n , H a m d e n , C T, U S A

Table 2.12. Table o f equipm ent

A x o c la m p 2B A x o n In s tru m e n ts , F o s te r C ity, C A , U S A M b - 2 A n e a d s ta g e (g a in x i u - c u rre n t p a s s in g ) a n a i-ib -.^ A n e a a s ia g e (gam x i, re co ra m g )

H a m ilto n 0 .0 2 5 m l s y rin g e use d to d is p la c e R N A an d ttie H a m ilto n 4 -w a y (4 -4 ) o p p o s in g va lve , fo r s w itc h in g th e p e rfu sio n

H a m ilto n , N e va d a , U S A

50 m l m o n o je c t s y rin g e s , use d in th e p e rfu s io n T y c o H e a lth c a re , G o s p o rt, U K C a p illa ry g la s s fo r p u llin g in je c tio n n e e d le s

D ru m m o n d N a n o je c t s yste m

L a s e r La b S y s te m s Ltd, U K

S in g le c h a n n e l fla t be d c h a rt re c o rd e r K ip p & Z o n e n , L in co ln , U K

L is t M e d ica l L /M -3 P -A e le c tro d e p u lle r (u s e d fo r p u llin g in je c tio n n e e d le s ). D a rm s ta d t, G e rm a n y

N a ris h ig e M F - 8 3 0 m ic ro fo rg e (u s e d fo r p o lis h in g in je c tio n n e e d le s ) N a ris h ig e M M -3 m ic ro m a n ip u la to rs (u s e fo r re c o rd in g )

N a ris h ig e P C -1 0 P a tch e le c tro d e p u lle r (u s e d fo r p u llin g re c o rd in g e le c tro d e s

N a ris h ig e , T o k y o , Ja p a n

N iko n S M Z 6 4 5 M ic ro s c o p e fo r o o c y te d is s e c tio n an d in je c tio n N iko n , T o k y o , Ja p a n

T e k tro n ix I D S 2 1 0 o s c illo s c o p e . T e k tro n ix , O R , U S A

N e u ro lo g s yste m D ig itim e r, H e rtfo rd s h ire , U K

P re a m p 1 and 8 p o le b e sse l filte r C J C e le c tro n ic s , L o n d o n , U K

D y m a x 30 pu m p. C h a rle s A u s te n P u m p s, S u rre y, U K

G ils o n P 20, P 2 0 0 a n d P I 0 0 0 m ic ro p ip e tte s G ils o n , W is c o n s in , U S A