Chapter 7 – General Discussion
7.6 Conclusions
FDORs are extremely diverse in mycobacteria, and include functional roles as quinone reductases, lipid saturases, heme oxygenases and biliverdin reductases that can contribute to mycobacterial persistence and pathogenesis. These functions are determined by their cofactor specificities and structural characterisation shows flexible active sites that allow promiscuous activity with a wide variety of substrates in some FDORs. Detailed analysis of the catalytic mechanism of the F420 dependent biliverdin
reductases suggest that it proceeds similar to the nicotinamide dependent reaction in the equivalent mammalian proteins. Finally, the abundance of F420 producing bacteria
indicates physiological roles of the FDORs in these organisms in addition to their involvement in mycobacterial persistence.
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