THE DARK REACTION

The dark reaction is also referred to as the light-independent reaction, the Calvin cycle, or carbon fixation. The reaction takes place in the stroma of the chloroplast.

In the dark reaction, the carbon from carbon dioxide is added to the five-carbon sugar ribulose bisphosphate (RuBP) to produce a six-carbon compound. This six-carbon sugar is immediately split into two three-carbon molecules, which in a chain reaction using the ATP and NADPH from the light reaction are modified to form glyceraldehyde 3-phosphate. The glyceraldehyde 3- phosphate can be synthesized into carbohydrates such as glucose, and it can also be synthesized back into ribulose biphosphate. One of the glyceraldehyde 3-phosphate molecules is made into carbohydrates, while the other molecules remain in the Calvin cycle to serve as raw materials for the next round of production.

Roots

The roots of a plant draw water and minerals from the soil and pass them upward through xylem and phloem to the stem and leaves. Roots are also responsible for storing the plant’s organic nutrients, which are passed downward from the leaves through the phloem. Radiating from the roots is a system of root hairs, which vastly increase the absorptive surface area of the roots. Roots also anchor the plant in the soil.

Growth in Vascular Plants

Vascular plants undergo two kinds of growth, primary growth and secondary growth. Primary growth occurs in the apical meristems, located at the tip of both root and shoot, and is mainly a growth of vertical length. The meristems are regions of rapid mitotic division, churning out cells like a factory. When a cell divides, one of its offspring moves down into the plant body, where it elongates, and the other remains in the meristem to divide again.

Secondary growth is a growth of thickness. Secondary growth is a product of two different, though related, tissues, which both fall under the umbrella-term lateral meristems. Vascular

cambium exists between xylem and phloem: on its inside the cambium produces what is known as secondary xylem; on its outside it forms secondary phloem. The primary xylem and phloem are pushed farther inward and outward. The vascular cambium is more productive during the growing seasons. During the rest of the year it creates little growth. This phenomenon creates distinct rings of growth, each ring representing a single growing season. By studying these rings, it is possible to calculate the age of a plant, and it’s even possible to determine the specific conditions of a particular growing season. The second lateral meristem is called cork cambium and is responsible for the formation of cork (bark), which replaces the epidermis to form the protective covering of shoot and root.

Controlling Growth: Plant Hormones

Plant growth is controlled by plant hormones, which influence cell differentiation, elongation, and division. Some plant hormones also affect the timing of reproduction and germination.

• Auxins. The primary function of the auxin hormones is to elongate plant cells in the stem. Auxins are also responsible for root development, secondary growth in the vascular cambium, inhibition of lateral branching, and fruit development.

• Kinins promote cell division and tissue growth in leaf, stem, and root. Kinins are also involved in the development of chloroplasts, fruits, and flowers. In addition, they have been shown to delay senescence (aging), especially in leaves, which is one reason that florists use cytokinins on freshly cut flowers.

• Gibberellins stimulate growth, especially elongation of the stem, and can also end the dormancy period of seeds and buds by encouraging germination. Additionally, gibberellins play a role in root growth and differentiation.

• Ethylene controls the ripening of fruits. It also contributes to the senescence of plants by promoting leaf loss and other changes. Ethylene can bring buds and seeds out of dormancy, initiate flower development, and promote radial (horizontal) growth in roots and stems.

• Inhibitors restrain growth and maintain the period of dormancy in seeds and buds. Plant Behavior: Tropisms

When people think about plants growing, they generally think of them growing straight up or growing wider. But plants also display other types of growth in response to the stimuli within their environment. These responses to stimuli are called tropisms and are controlled by plant hormones. There are three main tropisms:

• Phototropism is the tendency of a plant to move toward light. Phototropism results from the rapid elongation of cells on the dark side of the plant, which causes the plant to bend in the opposite direction.

• Gravitropism refers to a plant’s tendency to grow toward or against gravity. A plant that displays positive gravitropism will grow downward, toward the earth. A plant that displays negative gravitropism will grow upward, away from the earth. Most plants are negatively gravitropic. Gravitropism is also controlled by auxin. In a horizontal root or stem, auxin is concentrated in the lower half, pulled by gravity. In a positively gravitropic plant, this auxin concentration will inhibit cell growth on that lower side, causing the stem to bend downward. In a negatively gravitropic plant, this auxin concentration will inspire cell growth on that lower side, causing the stem to bend upward.

• Thigmotropism, a reaction to touch, causes parts of the plant to thicken or coil as they touch or are touched by environmental entities. Tree trunks, for instance, grow thicker when exposed to strong winds and vines tend to grow straight until they encounter a substrate to wrap around.

Photoperiodism

and, consequently, reproduce. In many plants, flowering is dependent on the duration of day and night; this is called photoperiodism.

All flowering plants have been placed in one of three categories with respect to photoperiodism: short-day plants, long-day plants, and day-neutral plants. Despite their names, however, scientists have discovered that it is the uninterrupted length ofnight rather than length of day that is the most important factor in determining when and whether plants will bloom. Short-day plants begin to bloom when the hours of darkness in a 24-hour period rise above a critical level, as when days shorten in the autumn. These plants include poinsettias, chrysanthemums, goldenrod, and asters. Long-day plants begin to flower when the duration of night decreases past a critical point, as when days lengthen in the spring and summer. Spinach, lettuce, and most grains are long-day plants. Finally, many plants are day neutral, which means that the onset of flowering is not controlled by photoperiod at all. These plants, which are independent both of night length and day length, include tomatoes, sunflowers, dandelions, rice, and corn.

Plant Reproduction

Plants can reproduce both asexually and sexually. Each type of reproduction has its benefits and disadvantages.

Asexual Reproduction

Through asexual reproduction, many plants can produce genetically identical offshoots (clones) of themselves, which then develop into independent plants. This process is also called vegetative propagation. The many modes of vegetative propagation include the production of specialized structures such as tubers, runners, and bulbs. Grafting is an artificial form of vegetative

propagation. The advantages to this kind of asexual reproduction, which can occur either naturally or artificially, stem from the fact that it can occur more rapidly than seed propagation and can allow a genetically superior plant to produce unlimited copies of itself without variation.

TUBERS:

As seen in potatoes, tubers are fleshy underground storage structures composed of enlarged parts of the stem. A tuber functions in asexual propagation as a result of the tiny scale leaves equipped with buds that grow on its surface. Each of these buds can form a new plant that is genetically identical to the parent.

RUNNERS:

Runners are slender, horizontal stems that spread outward from the main plant, such as those found on strawberry plants. Entirely new plants can develop from nodes located at intervals on the runners; each node can give rise to new roots and shoots.

BULBS:

Bulbs such as onions and tulips are roughly spherical underground buds with fleshy leaves extending from their short stems. Each bulb contains several other buds that can give rise to new plants.

GRAFTING:

In grafting, two young plants are joined, first by artificial means and then by tissue regeneration. Typically, a twig or bud is cut from one plant and joined to a rooted plant of a related species or variety. The twig or bud is called the scion, and the plant onto which is it grafted (and that provides the roots) is called the stock. The scion eventually develops into an entire shoot system. Grafting often allows horticulturalists to combine the best features of two different plants into one plant. Sometimes the stock and scion retain independent characteristics, and sometimes the stock alters the characteristics of the scion in some desirable way.

Sexual Reproduction in Plants

All plants undergo a life cycle that takes them through both haploid and diploid generations. The multicellular diploid plant structure is called the sporophyte, which produces spores through meiotic division. The multicellular haploid plant structure is called the gametophyte, which is formed from the spore and gives rise to the haploid gametes. The fluctuation between these diploid and haploid stages that occurs in plants is called the alternation of generations. The way in which the alternation of generations occurs in plants depends on the type of plant. In

nonvascular plants, the dominant generation is haploid, so that the gametophyte constitutes what we think of as the main plant. The opposite is true for tracheophytes, in which the diploid generation is dominant and the sporophyte constitutes the main plant. The SAT II Biology only deals with the specifics of the tracheophyte alternation of generations, though nonvascular plants have a similar life cycle.

The dominant phase in the tracheophyte life cycle is the diploid (sporophyte) stage. The gametophytes are very small and cannot exist independent of the parent plant. The reproductive structures of the sporophyte (cones in gymnosperms and flowers in angiosperms) produce male and female haploid spores: microspores (male) and megaspores (female). These spores give rise to similarly sexually differentiated gametophytes, which in turn produce gametes. Fertilization occurs when a male and female gamete join to form a zygote. The resulting embryo, encased in a seed coating, will eventually become a new sporophyte.