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Evaluation of Interface Complementarity

A m ajor com ponent of the docking algorithm to be presented

w ill be the use o f a soft potential to search for areas o f steric

co m p lem en tarity betw een the m olecular surfaces o f ho st and guest

p ro tein s. T h is search will only be e ffe c tiv e if the lo ck -a n d -k ey

binding m odel is largely correct. The level o f c o m p lem en tarity at

p ro te in /p ro te in in te rfac es can be ju d g e d by e x am in in g the three

know n antibody/lysozym e com plexes (Table 2.1).

T he n a tu r e o f the a n tib o d y /a n tig e n in te r f a c e has been

d escrib ed (M ian, et a l , 1991; Padlan, 1990; W illiam, et al., 1990). In o rd er to test fu rth er the steric co m p lem en tarity in this region a

v o lu m e c a l c u l a t i o n w as c a r r ie d o u t on e a c h o f th e th re e

antibody/lysozym e complexes. The m ethod used was that of Gellatly

& Finney (1982). A hypothetical solvent shell was placed around the

com plex. The shell com pletely surrounded the com plex but did not

seep into the antib o d y /ly so zy m e interface. The vo lu m e inside this

sh ell w as then d iv id ed betw een the atom s in the c o m p lex , in

proportion to their van der W aals radii. This involved finding nearest

n e ig h b o u rs to each atom and p la c in g a p la n e b e tw ee n th em ,

p e rp e n d icu lar to the line passing directly though the atom centres

and with distances between the plane and the atoms in proportion to

th eir van der W aals radii. M any such planes were created around

each atom until a closed polyhedron was formed. The volume of this

polyhedron was taken to be the volume occupied by the atom.

S t r u c t u r e Resolution (Â) PDB file A u t h o r

H yH E L -10 3 .0 PDB3HFM Padlan et al.

( 1 9 8 9 )

HyHEL-5 2 .5 PDB2HFL Sheriff et al.

( 1 9 8 7 )

D1.3 2 . 8 o b t a i n e d f r o m

Dr S. Phillips

Amit et al.

( 1 9 8 6 )

L y s o z y m e 2 . 0 PDB6LYZ Diamond et al.

( 1 9 7 4 )

Table 2.1

P r o te in s tr u c tu r e d a ta fo r th e th re e a n t i b o d y / ly s o z y m e

c om plexes and for the unbound form of lysozym e. The structures

have resolutions betw een 2Â and 3Â, sufficient to correctly assign

m ain -ch a in and sid e-ch ain structure. T h ree o f the stru c tu re s are

d e p o site d in the B ro o k h av en Protein D ataB an k (B e rn stein et al.,

O nce volumes have been assigned to each atom, it was possible

to find the mean volume occupied by each atom type. For each atom

type an expected volum e was calculated by averaging the volum e

re s u lts fo r several p ro tein s, n o t in c lu d in g the a n tib o d y /ly s o z y m e

com plexes. E xam ining the d ifferences betw een the volum es seen at

the interface and those seen on average allowed an estim ation of the

packing density relative to the protein core

T he results of the volume calculations are sum m arised in Table

2.2. A volum e calculation was perform ed for each atom within the

in te rfac e ( V o b s e r v e d ) * The volume calculation was also carried out on

unrelated proteins, and an average volum e occupied for each atom

ty p e, w as c alcu late d (Vexpect)- This p red ic te d v o lu m e was then

com pared to the expected volume for an atom of that type. Taking a

ratio , fo r each atom , o f calcu lated vo lu m e to e x p ec te d average

volum e gives a packing density.

V*

p a c k i n g d e n s i t y observed

rt

ex p ec t

f o r a t o m i o f t y p e t

This num ber is 1.0 for an atom that occupies the standard amount of

volume. Atom s that are too tightly packed occupy too little volume

and therefore have a packing density less then 1.0, w hereas atoms

w hich are too loosely packed have a pack in g density g reater than

1.0. The packing densities shown are averages of a group o f atoms.

T he g lo b al p ack in g d e n sity is the a v erag e o v er all atom s, the

interface packing density is the average for the interface atoms. The

standard deviation of the global packing densities is also shown. The

d ifferen c e s betw een the global and in terface pack in g den sities are

very m u ch sm aller than the standard d eviation for all structures.

C o m p lex Global Packing D e n sity I n t e r f a c e Packing Density S t a n d a r d D e v ia tio n H yH E L -10 1 . 0 1 1 . 0 0 0 .1 6 HyHEL-5 0 .9 9 1 . 0 2 0 .1 8 D1.3 1 . 0 1 1 . 0 0 0 .1 8 Table 2.2 T h e r e s u l t s o f th e v o l u m e c a l c u l a t i o n s on th e

antibody/lysozym e complexes. A global packing density, an interface

packing density and the global standard deviation of packing density

is given fo r each of the co m p lex es. The p a ck in g d e n sitie s are

m easu red rela tiv e to a set o f sixty eig h t well res o lv e d protein

s t r u c t u r e s .

This m eans that the interface region is as tightly packed as the

protein core. Thus, the interface region m ust exhibit the same degree

of steric com plem entarity as the protein core. These results are in

a g re em e n t w ith those o f C h o th ia & Jan in (1975) w ho exam ined

protein/protein interface regions and found them to be close packed.