This menu allows you to flush the instrument (you must first define the worktable; see
“The Worktable window” on page 117), drop DITIs (you must first define the worktable), reset the Genesis options Te-VacS, Te-MagS, Te-Shake, set the DITI position (you must first define the worktable), validate and start a script. You can also start a script at a specified line.
4.4.1 Flush Instrument
This menu item is used to flush the instrument (fill it with system liquid). Gemini prompts you for the volume and the location of the waste and cleaner on the worktable.
4.4.2 Drop DITIs
This menu item is used to drop DITIs directly without running a script. Gemini prompts you for the location of the DITI waste on the worktable.
Figure 4-6: Execute menu
4.4.3 Reset Options
This menu item is used to initialize the following hardware options for your GENESIS instrument manually. The hardware options are normally reset automatically at the appropriate time and a manual reset is only necessary under abnormal circumstances.
• Te-VacS
• Te-MagS
• Te-Shake
• Te-MO WRC
• Carousel
The GENESIS instrument is interrogated for the presence of these options and the Reset Options menu item will be inactive (gray) if none of the options are detected or if Gemini is running in simulation mode.
The following dialog box is shown if at least one of the options is present (the list of devices will change according to the installed options):
The device number is shown alongside the device name.
Notes Te-VacS
You can initialize the Te-VacS devices individually or all devices.
Te-MagS
You can initialize the Te-MagS devices individually or all devices. In addition, you can initialize the Te-MagS heaters individually.
Te-Shake
You can initialize the Te-Shake devices individually or all devices.
Figure 4-7: Reset Options dialog box
Te-MO WRC
You can initialize the Te-MO WRC devices individually. This flushes the WRC with washing liquid #1 for 20 s using 50% pump power. This ensures that the tubing and the wash carrier is filled with liquid and free of bubbles.
Carousel
You can initialize the carousels individually.
Click OK to initialize the selected device and close the dialog box. Click Cancel to close the dialog box without initializing.
4.4.4 Fill System (ActiveTip)
If your instrument is fitted with ActiveTips, this menu item is used to fill the liquid system.
A special procedure is necessary because of the very small inner diameter of the ActiveTips.
4.4.5 Switch on/off Doorlocks
This menu item is used to open or close the doorlocks manually. This menu item is only available if your instrument is fitted with doorlocks and is not available in simulation mode. The doorlocks are normally opened and closed automatically at the appropriate time and you should only need to operate the doorlocks manually in special situations (e.g. during instrument configuration). See also “Configuring the Doorlocks” on page 90.
4.4.6 Send Firmware Command
This menu item lets you send a firmware command directly to the instrument and is only intended for specialists. See the respective instrument manual for a list of firmware commands.
4.4.7 Set DITI Position
If you are using DITIs, Gemini stores the position of the last fetched DITI. When starting a new run, the Get DiTis command starts picking up DITIs at the next available position.
After loading new DITI racks onto the worktable, this menu item can be used to ensure that the next DiTi is fetched from position 1 rather than from the middle of the new rack.
Warning:
Please be careful when using this feature. Sending the wrong commands can damage the instrument or objects in its workspace, hurt yourself or other people.
You can specify the next location for each of the available DiTi types. You can also specify the next DiTi position during script runtime with the Set DiTi Position command.
DiTi type
Specify the DiTi type for which you want to specify the next location.
Next DiTi position
Specify the rack containing the next available unused DiTi of this type by clicking on the new DiTi tray in the Worktable window. Then specify the rack position (you should normally choose position 1).
4.4.8 Loading the Te-MO with racks
The Te-MO can be loaded with racks during script runtime using the RoMa. You can also load it manually before starting the script. Depending on their position in the TeMO, some of the sites may be difficult to access due to the position of the slides. This command is used to move a specified site to the RoMa exchange position (transfer position) manually to make it easier to exchange the rack. In this position, the LiHa or RoMa can access the specified site without colliding with the Te-MO pipetting head.
Figure 4-8: Set Next Available DiTi Position dialog box
Choose the required slide and site in the Sites panel and click Move in the RoMa Exchange Position panel to move the site to the exchange position.
When the Te-MO reaches the RoMa exchange position and you are using an APC (Active Positioning Carrier), click Open in the APC panel to release the rack. Click Close in the APC panel to lock the rack in position again.
4.4.9 Validate
See “Validating a script” on page 157.
4.4.10 Start
See “Starting a script” on page 158.
4.4.11 Pause
See “Pausing a script” on page 158.
4.4.12 Continue
See “Continuing a script” on page 158.
4.4.13 Abort
See “Stopping a script” on page 159.
4.4.14 Start at line ...
See “Starting a script at a certain line” on page 159.
Figure 4-9: Loading the Te-MO dialog box
4.4.15 Undefined Variables
See “Undefined Variables option” on page 159.