The graph shows the measured curves of the samples. A specific color is assigned to every sample. The color fields left of the sample table serve as color code.
The View menu provides options for the display of additional information:
• Presentation of the line of regression as continuous line in the same color as the sample curve
• Display of the limits of the regression range as broken vertical lines
• Display of a grid in the background
Displaying measured kinetic curves 7.4.1
If you select a single sample by a click on the colored field beside the table, its curve and, if this option was activated, also the corresponding line of regression are shown in colors in the graph, whereas all other curves appear in gray.
You can also define for every curve individually, whether it shall appear colored, gray or whether it shall be hidden. For that, mark the corresponding fields of the
"mark", "unmark" or "hide" columns of the table. The functions being active for the sample are marked by (x) in the field.
Fig. 62 Marking the curves for graphic presentation
Column Description
mark The curve of this sample appears colored.
unmark The curve appears in gray.
hide The curve is hidden.
The All check boxes below the columns facilitate marking. By activation one of the check boxes, the function specified in the column head is assigned to all samples simultaneously.
Scaling of kinetic curve presentation 7.4.2
Setting the scaling via entries in a dialog box
Activate the menu command View / Scaling.
This will bring up the Kinetics – Zoom dialog box.
Fig. 63 Kinetics – Zoom dialog box
In the corresponding textboxes, type the desired limits for abscissa and ordinate presentation. By a click on the [Apply] button, you can reset the original scaling of the graph.
Confirm your entries with [OK].
The selected section of the graph appears.
Interactive selection of the displayed section of the graph
Click on the button (top left above the graph).
In the graph, the mouse pointer turns into a full arrowhead.
With the mouse button held depressed, draw a rectangle across the section to be zoomed in.
When you release the mouse button, the selected section appears.
By a click on , the software automatically resets the original scaling of the graph.
7.5 Printing a kinetic analysis
Activate menu command File / Print.
This will bring up the dialog box for preparing the printout.
Fig. 64 Print Kinetics - Routine dialog box
Activate the check boxes of the items you want to include in the printout and choose the desired layout and printer options.
The functions of Page Layout and Printer Setup are described in Section
"Printing files" pg. 54.
Start the printout of the kinetic analysis by a click on [Print].
8 Biochemical Analysis (Bio Module)
The Bio module is integrated in the WinASPECT PLUS desktop and its functions are accessible via the Bio menu.
The Bio module allows the measurement of proteins, DNA and RNA and result analysis according to the following methods:
Method name Formula
Absorbance 260 nm x = A (260nm)
DNA Purity A260/A280 x = A(260nm) / A(280nm) Absorbance 260 nm, Factor 33 x[µg/ml] = A(260nm) * 33 Absorbance 260 nm, Factor 40 x[µg/ml] = A(260nm) * 40 Absorbance 260 nm, Factor 50 x[µg/ml] = A(260nm) * 50
Warburg and Christian (DNA) x[µg/ml] = (A(260nm)* 62.9) - (A(280nm)*36) Warburg and Christian (Protein) x[mg/ml] = (A(280nm)*1.55) - (A(260nm)*0.76) Absorbance 280 nm x = A(280nm)
Absorbance 205 nm, Factor 31 x[mg/ml] = A(205nm) * 31
Scopes Formula x[mg/ml] = ((A(280nm) / A(205nm))*120) + 27 Whitaker and Granum x[mg/ml] = (A(235nm) – A(280nm))/2.51 Absorbance 215nm, 225nm x[µg/ml] = (A(215nm) – A(225nm)) * 144 Kalb and Bernlohr x[µg/ml] = (A(230nm)* 183) - (A(260nm)*75.8) Absorbance 280 nm, Factor 1.38 x(mg/ml) = A(280 nm) * 1.38
Kalckar and Shafran x[mg/ml] = (A(280nm)* 1.45) - (A(260nm)*0.74) In addition, you can recall result records created with the Bio Module for later review and printout.
The preset path for files created with the Bio module is \\WinASPECT PLUS\Bio.
Result files are saved under the selected name with the following filename extensions:
"*.bio" Bio method file
"*.bios" Signed Bio method file
In the Bio module, only files that have been created with this module can be opened and edited. The evaluation of spectra that have already been measured and stored (“.dat” files) is not possible.
The measurement parameters used are saved together with the analytical results of a measurement series. When re-loading this file, the corresponding
measurement parameters are available for further measurements. The
measurement results will then be appended to the existing measurement record.
Activate the menu function Bio / Method.
On doing so, the WinASPECT PLUSbecomes inactive and the Method dialog box appears.
Bio / Method
Fig. 65 Method dialog box This dialog box has two tabs:
• Setup with the current measurement and analysis parameters
• Results with result analysis.
Functions of the menu bar of the Bio Module
The Method dialog box provides the following menu functions and buttons:
Menu function /
Button Description
New Starting new analyses.
Open Opening a file created with the Bio module.
The filename extension for files of the Bio module is "*.bio"
or “*.bios”.
Save Saves the results and settings under the currently valid filename.
Save as... Saves the results and settings to a new file.
Saving is via the Windows standard dialog box for saving files.
Print Starts the printout of results and settings.
Copy to clipboard Copies the results to the Windows clipboard.
Thus, the results are accessible to other Windows applications.
Close Closes the current Bio module session.
Setup Selection of the settings for an analysis with the Bio module.
Sign Document Providing the results with a signature (→ Section
"Electronic signature" p. 203).
View signatures Shows the existing signature of an open calibration file.
Audit Trail Shows the Audit Trail list.
Starts the measurement.
Starts the reference measurement.
Activating online help.
Closes the Bio module session and the Method dialog box.