5.4.1 The sample size may have been small to detect a significant difference in prevalence of pregnancy associated malaria between interventions because published baseline data for the risk of malaria infections in pregnant women was not available in the population. As such, the 40% used for sample size calculation was higher than the prevalence detected at baseline in all three treatment arms.
5.4.2 The methanol/water DNA extraction protocol used is very crude and may not yield good quality DNA for such important trials. This may have lowered chances of the procedure detecting low parasitaemia cases. The method was used because it very affordable and minimized the cost of running volumes of samples that were collected considering the multiple number of visits per participant.
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