This thesis highlights the development of live cell interferometry (LCI), a label-free, non-invasive, accurate, longitudinal, wide-field imaging technique that provides real-time measurements of cell biomass, morphology, motion and other biophysical properties. LCI was originally designed in a Michelson interferometer setup to perform low-throughput PSI measurements on single adherent cells. The first study was the quantification of cytoskeletal rearrangements in NIH/3T3 embryonic fibroblasts in response to mechanical stimulation.41 The platform application evolved to encompass fields in fundamental biology and clinical translation research. Topics such as regulation of cell mass during division, differentiation, growth were thoroughly investigated.27, 42 More therapeutically significant studies such as characterizing tumor cell drug response, tumor heterogeneity, tumor-reactivity of T-cells and correlative prediction of patient drug response emphasized the versatility of the technology in clinical applications.18, 26, 31, 43 This thesis explores the evolution of LCI in technologies and applications, as well as its future directions in pushing forward basic and translational research.
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