To plumb the binary solvent manager and sample manager:
1. Route the solvent inlet tubing attached to the in-line degasser and seal wash pump through the channel clips of the sample manager.
2. Continue routing the lines between the column heater door and hinge, through the detector's clip, and then place the lines into the solvent tray.
Routing solvent inlet lines
Exception: If your system uses a mass spectrometer positioned to the
right of the system stack, ensure that the column heater door is in the “away” (swung to the right) position. Then route the lines in front of the column heater door, not between the door and hinge.
Caution: To prevent contamination, wear particle-free, powder-free,
non-latex gloves when plumbing the binary solvent manager and sample manager.
Clip
3. Remove the two protective caps from the orange and white color coded wash lines.
4. Remove the two protective screws from the corresponding wash ports on the in-line degasser of the binary solvent manager.
5. From the sample manager, route the weak and strong needle wash lines, indicated by the orange and white labels, to their corresponding ports on the in-line degasser in the binary solvent manager. Finger tighten the knurled nut.
6. Locate the pre-installed Tygon® tubing running from the process waste port, and the PharMed® tubing running from the needle-clean system waste port (found on the lower drip dray of the sample manager), and route them through the pass-through of the upper binary solvent manager drip tray.
Pre-installed Tygon and PharMed tubing
TP02590
Process waste from sample manager (Tygon)
Needle clean system drainage waste (PharMed)
7. Connect the PharMed tubing to the front barbed fitting and the Tygon to the front boss fitting, both of which are on the lower binary solvent manager drip tray.
Location of various tubings and binary solvent manager mixer
8. Route the seal wash and solvent inlet line tubing attached to the in-line degasser through the channel clips of both the binary solvent manager and sample manager.
9. Continue routing the lines between the column heater door and hinge and through the detector’s clips. Then place the lines in the solvent tray. 10. Remove the protective O-ring from the stainless steel pump outlet
tubing.
TP02589
Tygon tubing from process waste
From backpressure regulator outlet Mixer
PharMed tubing from needle clean system waste
11. Seat the end of the tubing with the shorter compression screw into the binary solvent manager mixer outlet, and tighten the compression fitting using the 1/4-inch and 5/8-inch open-end wrenches.
Tip: When using new fittings, tighten 3/4-turn beyond finger-tight. For older or previously used fittings, tighten 1/4-turn beyond finger-tight.
Needle wash and pump outlet connections
12. Route the other end of the tubing through the channel clips to the sample manager injection valve.
TP02480
Strong needle wash Weak needle wash
Binary solvent manager mixer outlet
13. Remove the O-ring, and seat the end of the tubing with the longer compression screw into port 5 on the injection valve. Tighten the compression fitting using the 1/4-inch open-end wrench.
Injection valve connections
14. Remove the protective cover from the injector outlet tube, and then seat the tube and ferrule into port 6 of the injection valve. Tighten the compression fitting using a 1/4-inch open-end wrench.
Pump outlet tubing into port 5
Injector outlet/column stabilizer tubing into port 6
15. Slide a waste line over the barbed drain fitting located at the bottom of the binary solvent manager, and route it to a suitable waste container.
Attaching the waste line to the binary solvent manager
16. Route the degasser vent line to a suitable waste container.
Caution: To avoid distorting the drip tray or causing the drain cup
to leak, restrain the drain cup when attaching or removing the waste line.
Warning: To avoid releasing solvent vapors into the room, route the degasser vent line
• to a fume hood or other suitable exhaust system.
TP02479
Drip tray
Barbed drain fitting
Waste line
Drain cup
Correct positioning of waste and degasser vent lines
Caution: To avoid fluid backup, you must ensure proper drainage of
waste:
• Place the waste container below the system stack.
• Ensure that the waste and degasser vent lines do not crimp or bend. A crimp or bend may impede flow to the waste container. • Ensure the exit of the waste and degasser vent lines is not
immersed in waste solvent. If necessary, shorten each line so that no portion of it drops below the top of the waste container (see next figure). TP02709 Waste line Degasser vent line Waste line Degasser vent line Correct Incorrect
17. Attach a waste line to the barbed fitting on the filter drainage assembly (on the lower front side of the sample organizer), and route it to a suitable waste container.
Sample organizer drain
18. Route a waste line from the barbed fitting on the rear of the solvent tray to a suitable waste container.
19. Close the sample organizer door, sample manager door, sample manager fluidics tray, and the binary solvent manager door.