CAUTION LASER LIGHT DO NOT STARE INTO BEAM
PREDEFINED PANELS, PROTOCOLS, AND QC TEMPLATES
B
Table 2.3 Three-Color Application [4 PMT System] Panels and Protocols
Panel Name
Protocol Name
(and QC Template Name if applicable) Description
3 Color Auto Setup [4 PMT System] _A3CL Flow-Set [4 PMT]* _C1 FITC/RD1 CYTO-COMP* _C3 RD1/PC5 CYTO-COMP [3CL-4PMT]* _Q3CL 1 45-FITC/G1RD1/G1PC5 [4]* _Q3CL 3 45-FITC/4-RD1/3-PC5 [4]* _Q3CL 5 45-FITC/8-RD1/3-PC5 [4]* _Q3CL 7 45-FITC/19-RD1/3PC5 [4]* _Q3CL 9 45-FITC/56-RD1/3PC5 [4]*
_ Adjusts and monitors the Cytometer settings. _ Adjusts the compensation percentages. _ The _Q templates in this panel monitor the % positive, mean channel, and absolute counts (if using Flow-Count fluorospheres) for the pertinent regions in each protocol. Use the protocols to analyze control cells. Modify the panel and protocols as needed. The 3CL.qcs baseline file updates the Cytometer settings when running this panel.
N/A _A3ECD Flow-Set Adjusts and monitors Cytometer settings for three-color immunophenotyping using FITC, RD1, and ECD on either a 3 PMT or 4 PMT system.
N/A _Q3ECD 2 FITC/RD1/ECD [4]* Use this protocol to create additional 3 Color Auto Setup protocols for different antibody combinations of FITC, RD1, and ECD for control cells. Subsequently, additional _Q protocols can be added to the original 3 Color Auto Setup Panel, as your applications require. The 3CL.qcs baseline file updates the
Cytometer settings when running this protocol. N/A _Q3CL A4 FITC/RD1/PC5 [4]* Use this protocol to create additional 3 Color
Auto Setup protocols for different antibody combinations of FITC, RD1, and PC5 for control cells. Subsequently, additional _Q protocols can be added to the original 3 Color Auto Setup Panel, as your applications require. The 3CL.qcs baseline file updates the
Cytometer settings when running this protocol. N/A _Q3CL B2 G1-FITC/G1-RD1/G1-PC5 [4]* Add this protocol to the 3 Color Auto Setup
Panel if your application includes this antibody. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A _Q3CL B4 8-FITC/4-RD1/3-PC5 [4]* Add this protocol to the 3 Color Auto Setup Panel if your application includes this antibody. Modify the protocol as required for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
* Each _A, _C, and _Q protocol listed in this table has a corresponding QC template
predefined in the software. The QC template has the same name as the protocol and resides in the same directory.
3 Color Analysis [4 PMT System] 3CL 45-FITC/G1-RD1/G1-PC5 [4] 3CL 45-FITC/4-RD1/3-PC5 [4] 3CL 45-FITC/8-RD1/3-PC5 [4] 3CL 45-FITC/19-RD1/3-PC5 [4] 3CL 45-FITC/56-RD1/3-PC5 [4]
Use the 3 Color Analysis panel to analyze your three-color applications. Modify the panel and protocols as needed for your application.The 3CL.qcs baseline file updates the Cytometer settings when running this panel.
N/A 3ECD FITC/RD1/ECD [4] Use this protocol to create additional 3 Color Analysis protocols for different antibody combinations of FITC, RD1, and ECD. Subsequently, these additional protocols can be added to the original 3 Color Analysis Panel as your applications require. These protocols can be run alone or combined to create panels specific to your applications. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL FITC/RD1/PC5 [4] Use this protocol to create additional 3 Color Analysis protocols for different antibody combinations of FITC, RD1, and PC5. Subsequently, these additional protocols can be added to the original 3 Color Analysis Panel as your applications require. These protocols can be run alone or combined to create panels specific to your applications. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL 8-FITC/4-RD1/3-PC5 [4] Add this protocol to the 3 Color Analysis panel or use as a single protocol if your application includes analysis of this antibody combination. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL G1-FITC/G1-RD1/G1-PC5 [4] Add this protocol to the 3 Color Analysis panel or use as a single protocol if your application includes analysis of this antibody combination. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
PREDEFINED PANELS, PROTOCOLS, AND QC TEMPLATES
B
Table 2.4 Three-Color Application [3PMT System] Panels and Protocols
Panel Name
Protocol Name
(and QC Template Name if applicable) Description
3 Color Auto Setup [3 PMT System] _A3CL Flow-Set [3 PMT]* _C1 FITC/RD1 CYTO-COMP* _C3 RD1/PC5 CYTO-COMP [3CL-3PMT]* _Q3CL 0 45-FITC/G1RD1/G1PC5 [3]* _Q3CL 2 45-FITC/4-RD1/3-PC5 [3]* _Q3CL 4 45-FITC/8-RD1/3-PC5 [3]* _Q3CL 6 45-FITC/19-RD1/3PC5 [3]* _Q3CL 8 45-FITC/56-RD1/3PC5 [3]*
_ Adjusts and monitors the Cytometer settings. _ Adjusts the compensation percentages. _ The _Q Templates in this panel monitor the % positive, mean channel, and absolute counts (if using Flow-Count fluorospheres) for the pertinent regions in each protocol. Use these protocols to analyze control cells. Modify the panel and protocols as required for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this panel.
N/A _Q3ECD 1 FITC/RD1/ECD [3] 620 BP* Use this protocol to create additional 3 Color Auto Setup protocols for different antibody combinations of FITC, RD1, and ECD. Subsequently, additional _Q protocols can be added to the original 3 Color Auto Setup Panel, as your applications require. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A _Q3CL A3 FITC/RD1/PC5 [3] 675 BP* Use this protocol to create additional 3 Color Auto Setup protocols for different antibody combinations of FITC, RD1, and PC5. Subsequently, additional _Q protocols can be added to the original 3 Color Auto Setup Panel as your applications require. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A _Q3CL B1 G1-FITC/G1-RD1/G1-PC5 [3]* Add this protocol to the 3 Color Auto Setup Panel if your application includes this antibody. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A _Q3CL B3 8-FITC/CD4-RD1/CD3-PC5[3]* Add this protocol to the 3 Color Auto Setup Panel if your application includes this antibody. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol. 3 Color Analysis [3 PMT System] 3CL 45-FITC/G1-RD1/G1-PC5 [3] 3CL 45-FITC/4-RD1/3-PC5 [3] 3CL 45-FITC/8-RD1/3-PC5 [3]
Use the 3 Color Analysis panel to analyze your three-color applications. Modify the panel and protocols as needed for your application. The 3CL.qcs baseline file updates the Cytometer
* Each _A, _C, and _Q protocol listed in this table has a corresponding QC template
predefined in the software. The QC template has the same name as the protocol and resides in the same directory.
N/A 3ECD FITC/RD1/ECD [3] 620 BP Use this protocol to create additional 3 Color Analysis protocols for different antibody combinations of FITC, RD1, and ECD. Subsequently, these additional protocols can be added to the original 3 Color Analysis Panel, as your applications require. These protocols can be run alone or combined to create panels specific to your applications. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL FITC/RD1/PC5 [3] 675 BP Use this protocol to create additional 3 Color Analysis protocols for different antibody combinations of FITC, RD1, and PC5. Subsequently, these additional protocols can be added to the original 3 Color Analysis Panel, as your applications require. These protocols can be run alone or combined to create panels specific to your applications. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL 8-FITC/4-RD1/3-PC5 [3] Add this protocol to the 3 Color Analysis panel or use as a single protocol if your application includes analysis of this antibody combination. Modify the protocol as required for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
N/A 3CL G1-FITC/G1-RD1/G1-PC5 [3] Add this protocol to the 3 Color Analysis panel or use as a single protocol if your application includes analysis of this antibody combination. Modify the protocol as needed for your application. The 3CL.qcs baseline file updates the Cytometer settings when running this protocol.
PREDEFINED PANELS, PROTOCOLS, AND QC TEMPLATES
B
* Each _A, _C, and _Q protocol listed in this table has a corresponding QC template predefined in the software. The QC template has the same name as the protocol (but a different extension) and resides in the same directory.
* Each _A and _Q protocol listed in this table has a corresponding QC template predefined in the software. The QC template has the same name as the protocol and resides in the same directory.
Table 2.5 Four-Color Application Panels and Protocols
Panel Name
Protocol Name
(and QC Template Name if applicable) Description
4 Color Auto Setup _A4CL Flow-Set* _C1 FITC/RD1 CYTO-COMP* _C2 RD1/ECD CYTO-COMP* _C3 RD1/PC5 CYTO-COMP [4CL]* _C4 ECD/PC5 CYTO-COMP* _Q4CL FITC/RD1/ECD/PC5*
_ Adjusts and monitors the Cytometer settings. _ Adjusts the compensation percentages. _ The _Q Templates in this panel monitor the % positive, mean channel, and absolute counts (if using Flow-Count fluorospheres) for the pertinent regions in each protocol. Modify the panel and protocols as needed for your application. Use these protocols to analyze control cells. The 4CL.qcs baseline file updates the Cytometer settings when running this panel. 4 Color
Analysis
4CL FITC/RD1/ECD/PC5 Use the 4 Color Analysis panel to analyze your four-color applications. Modify the panel as required for your application. The 4CL.qcs baseline file updates the Cytometer settings when running this panel.
Table 2.6 DNA Application Panels and Protocols
Panel Name
Protocol Name
(and QC Template Name if applicable) Description
N/A _ADNA INDEX Control* Use this Autostandardization protocol to adjust the Cytometer settings for DNA analysis. Use the QC template to monitor the Cytometer settings. N/A _QDNA INDEX Control* Use this protocol to collect and monitor DNA
INDEX Control percentages and mean channels. The dna.qcs baseline file updates the Cytometer settings when running this protocol.
N/A DNA Analysis Use this protocol to analyze your DNA specimens. Modify as needed for your application. The dna.qcs file updates the Cytometer settings when running this protocol.