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Preparative PAGE Cells Catalog # Description

In document Electrophoresis and Blotting (Page 81-83)

Model 491 Prep Cell and Mini Prep Cell Pg 206

Model 491 Prep Cells

170-2926 Model 491 Prep Cell, 100/120 V, includes buffer recirculation pump, prep cell starter kit with protein standard 170-2927 Model 491 Prep Cell, 220/240 V

170-2928 Model 491 Prep Cell without Buffer Recirculation Pump Replacement Parts and Accessories for the Model 491 Prep Cell 170-2944 Prep Cell Casting Stand

170-2929 Buffer Recirculation Pump, 100/120 V 170-2930 Buffer Recirculation Pump, 220/240 V 170-2932 Small Gel Tube Assembly, 28 mm ID 170-2933 Large Gel Tube Assembly, 37 mm ID

170-2934 Cooling Finger Assembly, includes feedline connectors

170-2935 Buffer Circulation Tubing Kit, includes stopcock with tubing and connectors, 3 elution buffer circulation lines and connectors, and electrophoresis/cooling buffer circulation lines and connectors 170-2936 O-Ring Kits, 2

170-2937 Dialysis Membranes, precut, 5 170-2938 Frit Kit, includes support frit and elution frit

170-2939 Sample Application/Overlay Buffer Kit, includes sample loading guide, syringe with Teflon tubing 170-2940 Thumbscrews, 4

170-2969 Lid with Power Cables 161-5101 Prep Cell Starter Kit

161-0323 Prep Cell Starter Kit Protein Standard, 1 ml 170-2941 Elution Manifold Base

Mini Prep Cells

170-2915 Mini Prep Cell with Reagent Starter Kit 170-2908 Mini Prep Cell without Reagent Starter Kit Replacement Parts and Accessories for the Mini Prep Cell 170-2909 Gel Tubes, 2

170-2913 Sample Application/Purge Kit

170-2947 Peristaltic Pump Adaptor Kit, for 0.8 mm tubing 170-2948 Elution Frit Kit, with 5 dialysis membranes, MW cutoff 3,500 170-2911 Elution Frit Kit, with 5 dialysis membranes, MW cutoff 6,000 170-2912 Harvest Ring Assembly, includes Teflon elution collection tubing 170-2917 Mini Prep Cell Elution Chamber Top

170-2918 Mini Prep Cell Casting Stand 170-2916 Elution Manifold Base 800-7533 Lid with Cables, for mini prep cell

Preparative Electroelution Cells

Whole Gel Eluter and Mini Whole Gel Eluter Pg 207

Whole Gel Eluter

165-1251 Whole Gel Eluter with Harvesting Box, includes lid, electrodes, elution chamber core, base, roller, ruler, template, 75 pieces of lower filter paper, 50 pieces of upper filter paper, 50 sealing strips, 25 pieces of cellophane, application note; requires a vacuum source pulling 5" Hg for the harvesting box

165-1250 Whole Gel Eluter, includes lid, electrodes, elution chamber core, base, roller, ruler, template, 75 pieces of lower filter paper, 50 pieces of upper filter paper, 50 sealing strips, 25 pieces of cellophane, application note Whole Gel Eluter Accessories

165-1260 Harvesting Box

165-1270 Whole Gel Eluter Template 165-1275 Cellophane, 25 precut sheets 165-1277 Sealing Tabs, 50

165-1280 Lower Chamber Filter Paper, 21 x 21 cm, 75 precut sheets 165-1281 Upper Chamber Filter Paper, 21 x 21 cm, 50 precut sheets 170-2940 Thumbscrews, 4

Catalog # Description Mini Whole Gel Eluter

165-1256 Mini Whole Gel Eluter with Harvesting Box, includes lid, electrodes, elution chamber core, base, roller, ruler, template, 50 pieces of lower filter paper, 50 pieces of upper filter paper, 50 sealing tabs, 25 pieces of cellophane, application note; requires a vacuum source pulling 5" Hg for the harvesting box

165-1255 Mini Whole Gel Eluter, includes lid, electrodes, elution chamber core, base, roller, ruler, template, 50 pieces of lower filter paper, 50 pieces of upper filter paper, 50 sealing tabs, 25 pieces of cellophane, application note

Mini Whole Gel Eluter Accessories 165-1261 Mini Harvesting Box

165-1271 Mini Whole Gel Eluter Template 165-1276 Cellophane, 25 precut sheets 165-1278 Sealing Tabs, 50

165-1282 Lower Chamber Filter Paper, 9 x 10 cm, 50 precut sheets 165-1283 Upper Chamber Filter Paper, 5 x 6 cm, 50 precut sheets

Model 422 Electro-Eluter Pg 207

165-2976 Model 422 Electro-Eluter, includes electro-eluter module, membrane caps (MW cutoff 12,000–15,000), glass tubes, frits, silicone adaptors, grommets and stoppers, buffer tank, lid with power cables 165-2977* Model 422 Electro-Eluter Module, without buffer tank and lid

Accessories

165-2985 Membrane Caps, clear, MW cutoff 12,000–15,000, 12 165-2986 Membrane Caps, green, MW cutoff 3,500, 12 165-2987 Frits, 12

165-2978 Glass Tubes, 6 165-2981 Silicone Adaptors, 6 165-1988 Grommets and Stoppers, 8

* Module can be used with the discontinued Mini-PROTEAN® 3 cell. If you do not own a Mini-PROTEAN 3 cell,

order Model 422 electro-eluter catalog #165-2976.

www.bio-rad.com/horizontalelectro Electrical leads Safety lid Electrical cables Gel casting gates Fixed-height comb Mini-Sub cell GT base

Safety lid removal tab Comb slots Banana plug/ electrode wire assembly UV-transparent gel tray Fluorescent ruler Gel stage

Components of the Mini-Sub cell GT cell.

Sub-Cell Family Selection Guide

Wide Mini-Sub Sub-Cell Sub-Cell

Mini-Sub Cell GT* Cell GT** Sub-Cell GT Model 96 Model 192

Cell size (W x L x H) 9.2 x 25.5 x 5.6 cm 17.8 x 25.5 x 6.8 cm 18 x 40.5 x 9.4 cm 29 x 30 x 9 cm 29 x 40 x 9 cm Gel tray sizes (outside 7 x 7 cm 15 x 7 cm 15 x 10 cm 25 x 10 cm 25 x 10 cm dimensions, W x L) 7 x 10 cm 15 x 10 cm 15 x 15 cm 25 x 15 cm 25 x 15 cm

15 x 20 cm 25 x 20 cm

15 x 25 cm 25 x 25 cm

ReadyAgarose gels Yes (mini format Yes (wide mini No No No

accommodated 8-, 12-, 2 x 8-well) and 96 Plus formats)

Sample 8–30*** 10–60*** 1–120† 24–96*** 24–192

throughput

Base buffer ~270 ml ~650 ml ~1 L ~2 L ~3 L

volume

Buffer recirculation No No No Yes Yes

Bromophenol ~4.5 cm/hr ~4.5 cm/hr ~3.0 cm/hr ~6.2 cm/hr ~5.2 cm/hr

blue migration (at 75 V) (at 75 V) (at 75 V) (at 200 V) (at 200 V)

* The mini ReadySub-Cell GT cell is a Mini-Sub cell GT cell dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm; sample throughput is 8, 12, or 2 x 8. This cell does not include casting gates, tray, or combs. ** The wide mini ReadySub-Cell GT cell is a wide Mini-Sub cell GT cell dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm; sample throughput is 20, 32, 2 x 32, or 4 x 26. This cell does not include casting gates, tray, or combs. *** Sample throughput value assumes 1–2 combs per gel. † Sample throughput value assumes 1–4 combs per gel.

In document Electrophoresis and Blotting (Page 81-83)

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