Hamunyare Ndwabe*, Manikandan K And Lakshmi K.S
Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Kattankulathur – 603 203, Tamilnadu.
ABSTRACT
Objective: A simple, specific, accurate, cost effective and precise and stability indicating HPTLC method was developed and validated for the determination of Nitrendipine in pure and doage form.Methods: Stability test were done through exposure of the analyte solution to Acid, Alkali, Oxidiative, Photo-degradation, Thermal degradation. The chromatographic separation was carried out using Camag Linomat V semi-Automated sample applicator with TLC Scanner III. Stationary Phase consisting of TLC plates (Merck) pre coated with silica gel 60F254 on Aluminum Sheets was used. Mobile phase comprising of Acetonitrile: Ethyl acetate: water (6:3:1 v/v/v).Results: It is highly labile to alkaline and acid hydrolysis compared to oxidation. The correlation coefficient (r2=0.9986) with slope and Intercept of 2875.3 and 344.43 respectively. The LOD and LOQ were found to be 0.0260 and 0.0788 respectively. The % RSD was found to be 1.510%. The percentage recovery was found to be 100.06%. Densitometric peak purity results indicated the absence of co-eluting peaks with the main peak of Nitrendipine, which demonstrated the specificity of assay method.Conclusion: The proposed HPTLC method was validated and can be applied for the determination of Nitrendipine in pharmaceutical formulations and used in routine laboratories.
Key words: Nitrendipine, HPTLC, ICH Guidelines, stability indicating, method development
Organised by: College of Pharmacy, Mother Theresa Post Graduate Research
Institute of Health Sciences, Puducherry Page 132
EMULGEL: A RECENT ADVANCE IN TOPICAL DRUG DELIVERY
S. Siventhi*1, Padmapriya2
1. B.pharm final year, MTPG&RIHS, Puducherry.
2. Assist prof.dept of pharmaceutics, MTPG&RIHS, Puducherry.
ABSTRACT
Dermatological products are diverse in formulation and range in consistency from liquid to powder but the most popular products are semisolid preparation. One among them is the transparent gels which has widely used in both in cosmetics and in pharmaceutical preparations. Gels are created by entrapment of large amounts of aqueous or hydro alcoholic liquid in a network of colloidal solid particles and generally provide faster drug release compared with conventional ointments and creams. A major limitation in the gel is the difficulty in delivery of hydrophobic drugs. So to overcome this limitation, an emulgels are prepared. Emulgel is an emulsion based approach used for delivery of hydrophobic therapeutic moiety and also has the unique properties of gels. When gels and emulsions are used in combined form the dosage form are referred as emulgel. The presence of a gelling agent in water phase converts a classical emulsion into emulgel. Various penetration enhancers can potentiate the effect. The emulgel for dermatological use has several favorable properties such as being thixotropic, greaseless, easily, spreadable, easily removable, emollient, and non-staining, water-soluble, longer shelf life, bio-friendly, transparent and pleasing appearance. These emulgel are having major advantages on novel vesicular systems as well as on conventional systems in various aspects. Thus emulgel can be considered as better topical drug delivery systems for the treatment of dermatological ailments over present conventional systems available in market.
KEYWORDS: Emulgels, Gelling agent, Penetration enhancers, Topical drug delivery.
Organised by: College of Pharmacy, Mother Theresa Post Graduate Research
Organised by: College of Pharmacy, Mother Theresa Post Graduate Research
Institute of Health Sciences, Puducherry Page 134
FORMULATION AND EVALUATION OF HERBAL ANTI-ACNE GEL
T. Shri Vijaya Kirubha, R. Senthamarai, P.Balasubramanian, K. Akila*
Department of Pharmacognosy, Periyar College of Pharmaceutical Sciences, Tiruchirappalli, TamilNadu, India
ABSTRACT
Acne is an inflammatory disease of sebaceous follicles of skin. The present study was conducted to formulate and evaluate the topical anti acne formulation. Topical formulations (Gel ) have been developed containing hydro-alcoholic extracts of Ocimum basilicum, Acalypha indica, Curcuma longa and Aloe vera concentrate gel powder which have been reported individually for their antimicrobial, anti inflammatory and antioxidant activities. The developed formulation was examined for physical parameters such as colour, consistency, odour, pH, viscosity, spreadability, extrudability and washability.In vitro antibacterial activity was performed for the developed formulations using agar well diffusion method against Propionibacterium acne - a causative organism for acne and which is frequently involved in acne inflammation. The measured zones of inhibitions of the formulations were compared with standard antibiotic (tetracycline), standard marketed topical herbal preparation for acne and active ingredients of the formulations.Results of the investigation showed that formulation 5 has greater antibacterial activity (zones of inhibition >11 mm) than other formulations and which is comparable to that of standard marketed topical herbal preparation (zone of inhibition > 13 mm). Keywords: Acne, In vitro antibacterial activity, Propionibacterium acne, Zone of inhibition
Organised by: College of Pharmacy, Mother Theresa Post Graduate Research
Institute of Health Sciences, Puducherry Page 135
STANDARDISATION OF POLYHERBAL FORMULATION – STONIL
CAPSULES
R. Senthamarai, T. Shri Vijaya Kirubha, P.Balasubramanian, N. Firose Banu* Department of Pharmacognosy, Periyar College of Pharmaceutical Sciences, Tiruchirappalli,
TamilNadu, India ABSTRACT
Herbal medicines are in great demand in developed and developing countries due to their wide range of biological activities, higher safety margins and cost effectiveness. The quality assessment of herbal formulations is of paramount importance in order to justify their acceptability in modern system of medicine. In the present study, a polyherbal formulation – Stonil capsules recommended for the management of renal stones which consist of leaves of Tribulus terrestris (Zygophyllaceae), leaves and roots of Bergenia ligulata (Saxifragaceae), leaves of Cinnamomoum camphora (Lauraceae) and Shilajit ( a mineral drug) was selected and investigated to standardize the polyherbal formulation in accordance to World Health Organization (WHO) norms and standard laboratory procedures. The formulation was evaluated for their organoleptic characters, physico-chemical and phytochemical parameters including spectral analysis and High Performance Thin Layer Chromatography (HPTLC) studies.The selected polyherbal formulation - Stonil capsule complied with the standards recommended by the Indian Pharmacopoeia and WHO standards. The present study is likely to help the Quality Assurance of Polyherbal formulations and in development of standard parameters.
Keywords:Polyherbal formulation, Stonil capsule.
Organised by: College of Pharmacy, Mother Theresa Post Graduate Research
Institute of Health Sciences, Puducherry Page 136